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[186050_s_at]

Description F55C7.7D /REP_DB=WormBase Gene ID /WP=CE19467 /TR=O61528 /GB=AAC71111.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 533
1 2 3 4 range
A 751.7
(3)
P 		291.2
(-2.6)
P 		259.3
(-3)
P 		266.3
(-2.9)
P 		492
B 688.4
(3)
P 		339.4
(-2.1)
P 		277.3
(-3)
P 		339.5
(-2.1)
P 		411
C 791.9
(3)
P 		399.4
(-1.7)
P 		293.8
(-3)
P 		307.4
(-2.8)
P 		498
avr 744
(3) 		343.3
(-2.1) 		276.8
(-3) 		304.4
(-2.6) 		467

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 570
1 2 3 4 5 6 range
A 520.9
(2.1)
P 		575.7
(3)
P 		266.3
(-2.3)
P 		228.1
(-3)
P 		249.6
(-2.6)
P 		264.5
(-2.4)
P 		348
B 646.2
(3)
P 		525.5
(1.3)
P 		316.8
(-1.7)
P 		305.5
(-1.9)
P 		230.1
(-2.9)
P 		225.3
(-3)
P 		421
C 756
(3)
P 		462.7
(-0.1)
P 		311
(-1.7)
P 		185.7
(-3)
P 		207.2
(-2.8)
P 		227.6
(-2.6)
P 		570
avr 641
(3) 		521.3
(1.3) 		298
(-2) 		239.8
(-2.8) 		229
(-3) 		239.1
(-2.9) 		412
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 235
1 2 3 4 range
A 273.7
(3)
P 		63.2
(-2.4)
P 		67.8
(-2.3)
P 		39
(-3)
P 		235
B 219.5
(3)
P 		83.8
(-2.2)
P 		64.3
(-3)
P 		72.3
(-2.7)
P 		155
C 262.1
(3)
P 		114.8
(-1.2)
P 		102.4
(-1.6)
P 		51.8
(-3)
P 		210
avr 251.8
(3) 		87.3
(-2) 		78.2
(-2.3) 		54.4
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 243
1 2 3 4 5 6 range
A 96
(-1.4)
P 		204.7
(3)
P 		58
(-3)
P 		57.9
(-3)
P 		75.3
(-2.3)
P 		64.4
(-2.7)
P 		147
B 224.8
(3)
P 		174.9
(1)
P 		76.9
(-3)
P 		80.9
(-2.8)
P 		93.5
(-2.3)
P 		84
(-2.7)
P 		148
C 280.2
(3)
P 		141.2
(-0.4)
P 		79.2
(-2)
P 		37.3
(-3)
P 		67
(-2.3)
P 		76.2
(-2)
P 		243
avr 200.3
(3) 		173.6
(1.9) 		71.4
(-2.5) 		58.7
(-3) 		78.6
(-2.2) 		74.9
(-2.3) 		142
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186050_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.151
Sequence Type Exemplar sequence
Representative Public ID F55C7.7D NCBI
Target Description F55C7.7D /REP_DB=WormBase Gene ID /WP=CE19467 /TR=O61528 /GB=AAC71111.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026325(11), NM_001026327(11), NM_001026328(11), NM_001026329(11), NM_001026330(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026325 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
NM_001026327 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
NM_001026328 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
NM_001026329 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 11/11 None
NM_001026330 NCBI Caenorhabditis elegans UNCoordinated family member (unc-73) (unc-73) mRNA, complete cds. 9/11 None
SNAP00000014627 ENSEMBL cdna:SNAP chromosome:CEL140:I:4002333:4004842:-1 11/11 None
GENEFINDER00000014631 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4002333:4008190:-1 11/11 None
F55C7.7a ENSEMBL cdna:known chromosome:CEL140:I:3999350:4031628:-1 gene:F55C7.7 11/11 A 
F55C7.7c ENSEMBL cdna:known chromosome:CEL140:I:3999350:4020543:-1 gene:F55C7.7 11/11 A 
F55C7.7d ENSEMBL cdna:known chromosome:CEL140:I:4001623:4008337:-1 gene:F55C7.7 11/11 None
F55C7.7f ENSEMBL cdna:known chromosome:CEL140:I:4001631:4031628:-1 gene:F55C7.7 10/11 A 
F55C7.7e ENSEMBL cdna:known chromosome:CEL140:I:4001631:4011586:-1 gene:F55C7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171988 6 5 NM_001026325 172976_s_at (11), 186050_s_at (11), 190347_s_at (11), 193621_s_at (11)
NM_001026326 190347_s_at (11)
NM_001026327 172976_s_at (11), 186050_s_at (11), 193621_s_at (11)
NM_001026328 186050_s_at (11)
NM_001026329 186050_s_at (11)
NM_001026330 186050_s_at (9), 190347_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4002332-4008190(-) 100.0 100.0
Public Domain and Genome References
Ensembl F55C7.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71108 Uncoordinated protein 73, isoform a [Caenorhabditis elegans] ref|NP_001021496.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12931.1| guanine nucleotide exchange factor UNC-73A [Caenorhabditis elegans] 0.0
blast AAT68892 Uncoordinated protein 73, isoform f [Caenorhabditis elegans] ref|NP_001021501.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast AAC71109 Uncoordinated protein 73, isoform b [Caenorhabditis elegans] ref|NP_001021497.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] gb|AAC12932.1| guanine nucleotide exchange factor UNC-73B [Caenorhabditis elegans] 0.0
blast AAC71110 Uncoordinated protein 73, isoform c [Caenorhabditis elegans] ref|NP_001021498.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast CAE66729 Hypothetical protein CBG12078 [Caenorhabditis briggsae] 0.0
blast AAC71111 Uncoordinated protein 73, isoform d [Caenorhabditis elegans] ref|NP_001021499.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
blast AAL06029 Uncoordinated protein 73, isoform e [Caenorhabditis elegans] ref|NP_001021500.1| UNCoordinated family member (unc-73) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.7E-9
Pfam IPR000219 EMBL-EBI DH domain 2.4E-18
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Pfam IPR000219 EMBL-EBI DH domain 1.3E-30
Sequence
Target Sequence 
>C. ELEGANS:186050_S_AT
gacaaaagtctctcagctcaaggcaaacttattcatcagggaacactgcagatcagtgaa
tcgatagccggaaacgtacaaaaaccgaaagatcgacgaattttcttgtttgagcagtcg
gcgatcatcgcagatcacattccgccgaagaaggagttcggaaatcccacgtacattttt
aagagtcaatttatggtaaacaaaatggtgttcgagccaaatgtgccggatgatccgctt
cgattcgtcatcaagagctccgatccaacacagccgacaagcttcattgcgaatgcacaa
tcacaagaggagaaggacgaatggaatcgcaaaatgagcgagctactcgatcaacaaaaa
cgattgctcgcagctcttgtggatccgagacgatacaacgatatgtccagcggaatggga
gatctgagtttaggtgcccctggaggtgacaggagaggcgctccatcatcagcaaacaga
tcagcagcatcatcatcgaaaaagccagcagaatctccgaaaaaagagggaaaatcaaaa
tcgtcgctgttctcgtttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAAAGTCTCTCAGCTCAAGGCA 702 365 1063 Antisense
GTTTGAGCAGTCGGCGATCATCGCA 383 451 1170 Antisense
CGATCATCGCAGATCACATTCCGCC 526 247 1184 Antisense
GGTGTTCGAGCCAAATGTGCCGGAT 663 497 1269 Antisense
TGTGCCGGATGATCCGCTTCGATTC 207 555 1284 Antisense
GATTCGTCATCAAGAGCTCCGATCC 242 433 1304 Antisense
AACACAGCCGACAAGCTTCATTGCG 248 135 1329 Antisense
AAAACGATTGCTCGCAGCTCTTGTG 561 131 1419 Antisense
AGATCTGAGTTTAGGTGCCCCTGGA 654 57 1482 Antisense
GACAGGAGAGGCGCTCCATCATCAG 322 369 1510 Antisense
CAAAATCGTCGCTGTTCTCGTTTGG 208 191 1598 Antisense
Sequence Source Affymetrix Proprietary Database