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[186048_at]

Description F25E5.10 /REP_DB=WormBase Gene ID /WP=CE07144 /TR=O76654 /GB=AAG24070.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 28      4grp_total > 37      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 109.7
(3)
A 		82
(1.3)
A 		20.8
(-2.5)
A 		12.1
(-3)
A 		98
B 71.3
(3)
A 		29.4
(-1.4)
A 		13.9
(-3)
A 		22.2
(-2.1)
A 		57
C 22.2
(-3)
A 		57.9
(1.4)
A 		32.2
(-1.8)
A 		70.6
(3)
A 		48
avr 67.7
(3) 		56.4
(1.5) 		22.3
(-3) 		35
(-1.3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 17.7
(-1.4)
A 		9.1
(-3)
A 		25.6
(0.1)
A 		12.6
(-2.3)
A 		18.6
(-1.2)
A 		41.2
(3)
A 		32
B 48.1
(3)
A 		21.6
(-1.6)
A 		17.4
(-2.3)
A 		31.8
(0.2)
A 		22.1
(-1.5)
A 		13.2
(-3)
A 		35
C 53.7
(2.6)
A 		56.9
(3)
A 		9.2
(-3)
A 		13.6
(-2.4)
A 		37.9
(0.6)
A 		13.6
(-2.4)
A 		48
avr 39.8
(3) 		29.2
(0.2) 		17.4
(-3) 		19.3
(-2.5) 		26.2
(-0.6) 		22.7
(-1.6) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 39.9
(3)
A 		17.8
(-0.5)
A 		5.4
(-2.4)
A 		1.8
(-3)
A 		38
B 22.7
(3)
A 		7.3
(-1.7)
A 		3.2
(-3)
A 		4.7
(-2.5)
A 		20
C 7.4
(-3)
A 		16.6
(3)
A 		11.2
(-0.5)
A 		11.9
(-0.1)
A 		9
avr 23.3
(3) 		13.9
(-0.3) 		6.6
(-2.8) 		6.1
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 3.3
(-2.9)
A 		3.3
(-2.9)
A 		5.6
(-0.9)
A 		3.2
(-3)
A 		5.6
(-0.9)
A 		10
(3)
A 		7
B 16.7
(3)
A 		7.2
(-1.6)
A 		4.2
(-3)
A 		8.4
(-1)
A 		9
(-0.7)
A 		4.9
(-2.7)
A 		13
C 19.9
(3)
A 		17.4
(2.1)
A 		2.3
(-3)
A 		2.7
(-2.9)
A 		12.3
(0.4)
A 		4.6
(-2.2)
A 		18
avr 13.3
(3) 		9.3
(0.4) 		4
(-3) 		4.8
(-2.5) 		9
(0.2) 		6.5
(-1.4) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186048_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12221
Sequence Type Exemplar sequence
Representative Public ID F25E5.10 NCBI
Target Description F25E5.10 /REP_DB=WormBase Gene ID /WP=CE07144 /TR=O76654 /GB=AAG24070.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072515(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072515 NCBI Caenorhabditis elegans F25E5.10 (F25E5.10) mRNA, complete cds. 11/11 None
SNAP00000010680 ENSEMBL cdna:SNAP chromosome:CEL140:V:7455538:7456910:-1 11/11 None
GENEFINDER00000010692 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7455538:7456910:-1 11/11 None
F25E5.10 ENSEMBL cdna:known chromosome:CEL140:V:7455538:7456910:-1 gene:F25E5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7455538-7456911(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E5.10
Entrez Gene 179134 Entrez gene
SwissProt O76654 EMBL-EBI
WormBase CE07144 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4295 trypsin activity inferred from electronic annotation QuickGO AmiGO
16812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG24070 Hypothetical protein F25E5.10 [Caenorhabditis elegans] ref|NP_504916.1| F25E5.10 [Caenorhabditis elegans] 0.0
blast CAE74161 Hypothetical protein CBG21833 [Caenorhabditis briggsae] 6.0E-90
blast CAE57815 Hypothetical protein CBG00839 [Caenorhabditis briggsae] 1.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR001254 EMBL-EBI Peptidase S1, chymotrypsin 1.9E-39
Sequence
Target Sequence 
>C. ELEGANS:186048_AT
caaggaggttccatgcaagcacccatcagaagatcttttctgtgtgactgccgaaaaccc
actttgtaatggagactttggaggtgccgccgtgagtaagttccatggatcagttcgggc
ttacggtgtctatgtggatggcccatatgagtgtaacaaggcaaataggagaaccgttta
tacatttgctaacatgacaaaactagccgagtccctctgtgatgtaactggtatctgcgc
tccataccttccaagtcaacaaactaccgtgacgactacaattactccagttacatccat
cgatcataccaatactactccaaaccctggatcattcactacaggatcaggtactgtaac
gggttcagatggaacttgccccggaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGGAGGTTCCATGCAAGCACCCA 621 181 687 Antisense
GAAGATCTTTTCTGTGTGACTGCCG 65 339 715 Antisense
GCCGCCGTGAGTAAGTTCCATGGAT 608 285 772 Antisense
ATCAGTTCGGGCTTACGGTGTCTAT 519 27 795 Antisense
CCGAGTCCCTCTGTGATGTAACTGG 516 257 893 Antisense
ATGTAACTGGTATCTGCGCTCCATA 43 49 908 Antisense
GCTCCATACCTTCCAAGTCAACAAA 102 299 925 Antisense
ACCGTGACGACTACAATTACTCCAG 352 89 952 Antisense
ATTACTCCAGTTACATCCATCGATC 147 13 967 Antisense
ATCGATCATACCAATACTACTCCAA 696 35 985 Antisense
TTCAGATGGAACTTGCCCCGGAAAC 665 681 1050 Antisense
Sequence Source Affymetrix Proprietary Database