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[186046_at]

Description T05B4.12 /REP_DB=WormBase Gene ID /WP=CE13215 /TR=O16421 /GB=AAC69052.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2441
1 2 3 4 range
A 9.2
(-3)
A 		74.3
(-2.8)
A 		75.8
(-2.8)
A 		2280.2
(3)
P 		2271
B 63.6
(-2.9)
A 		48.7
(-3)
A 		36.4
(-3)
A 		2449.9
(3)
P 		2414
C 52.4
(-2.9)
A 		22.8
(-3)
A 		53.8
(-2.9)
A 		2041.6
(3)
P 		2019
avr 41.7
(-3) 		48.6
(-3) 		55.3
(-3) 		2257.2
(3) 		2216

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2783
1 2 3 4 5 6 range
A 7.5
(-3)
A 		61
(-2.9)
A 		18.3
(-3)
A 		68.5
(-2.9)
A 		508.6
(-1.9)
P 		2789.4
(3)
P 		2782
B 6.2
(-3)
A 		39.3
(-2.9)
A 		61.9
(-2.8)
A 		80.2
(-2.8)
A 		451.9
(-1.6)
P 		1890.9
(3)
P 		1885
C 47.4
(-2.9)
A 		42.5
(-2.9)
A 		59.2
(-2.9)
A 		19.7
(-3)
A 		636.6
(-1)
P 		1848.4
(3)
P 		1829
avr 20.4
(-3) 		47.6
(-2.9) 		46.5
(-2.9) 		56.1
(-2.9) 		532.4
(-1.6) 		2176.2
(3) 		2156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 519
1 2 3 4 range
A 3.3
(-3)
A 		16.1
(-2.8)
A 		19.8
(-2.7)
A 		333.8
(3)
P 		331
B 20.3
(-2.9)
A 		12
(-3)
A 		8.4
(-3)
A 		522.1
(3)
P 		514
C 17.3
(-2.8)
A 		6.6
(-3)
A 		18.7
(-2.8)
A 		344.2
(3)
P 		338
avr 13.6
(-3) 		11.6
(-3) 		15.6
(-2.9) 		400
(3) 		388

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 704
1 2 3 4 5 6 range
A 1.4
(-3)
A 		21.7
(-2.8)
A 		4
(-3)
A 		17.4
(-2.9)
A 		153.5
(-1.7)
P 		679.1
(3)
P 		678
B 2.1
(-3)
A 		13.1
(-2.9)
A 		15
(-2.9)
A 		21.2
(-2.8)
A 		183.6
(-1.5)
P 		705.1
(3)
P 		703
C 17.6
(-2.9)
A 		13
(-2.9)
A 		15.1
(-2.9)
A 		4
(-3)
A 		206
(-1)
P 		618.8
(3)
P 		615
avr 7
(-3) 		15.9
(-2.9) 		11.4
(-3) 		14.2
(-2.9) 		181
(-1.4) 		667.7
(3) 		661
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186046_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15410
Sequence Type Exemplar sequence
Representative Public ID T05B4.12 NCBI
Target Description T05B4.12 /REP_DB=WormBase Gene ID /WP=CE13215 /TR=O16421 /GB=AAC69052.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071748(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071748 NCBI Caenorhabditis elegans T05B4.12 (T05B4.12) mRNA, complete cds. 11/11 None
SNAP00000031065 ENSEMBL cdna:SNAP chromosome:CEL140:V:4123142:4124675:-1 11/11 None
GENEFINDER00000031071 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4123142:4124675:-1 11/11 None
T05B4.12 ENSEMBL cdna:known chromosome:CEL140:V:4123142:4124675:-1 gene:T05B4.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4123141-4124675(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T05B4.12
Entrez Gene 188107 Entrez gene
SwissProt O16421 EMBL-EBI
WormBase CE13215 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69052 Hypothetical protein T05B4.12 [Caenorhabditis elegans] ref|NP_504149.1| T05B4.12 [Caenorhabditis elegans] 1.0E-125
blast CAE65454 Hypothetical protein CBG10419 [Caenorhabditis briggsae] 1.0E-104
blast AAC69054 Hypothetical protein T05B4.3 [Caenorhabditis elegans] ref|NP_504152.1| T05B4.3 [Caenorhabditis elegans] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 15.0
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.1E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.5E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.2E-13
Sequence
Target Sequence 
>C. ELEGANS:186046_AT
atgcttcttgccaggttctttatgctgccccggacaccctatacccagctgctggaaatg
atgctgagagagcttatgcttgctacacaaccgccacagctaccccagctgctgttgtgg
ctgacatgaagactgctgcaattgcatcttgtgcaaaaacttgcggattctgctgcctaa
ctgacgactacaattgtgccaatgctgcttacccacgactcaactgcgctacaatcacca
aagctcaatgtaactcagtcacctggagaaccatcatcgctacagactgcccatctgcat
gcggattctgtaactctggtggatgcgtcgatgccgtcaccaactgcggaaacgacttga
gcatctgtaacactgttggaatgcaaaccttcgtcaatcaatactgccaaaagacttgcg
gaagatgcccatcaaccaccgcatcatctggatctgtcacctctggcacctgcacctcct
acgccgccgactcatcaacctcatgtacctcctgggctaagaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTTCTTGCCAGGTTCTTTATGC 407 43 149 Antisense
GAGCTTATGCTTGCTACACAACCGC 152 389 218 Antisense
GACTGCTGCAATTGCATCTTGTGCA 206 375 279 Antisense
TGCTGCCTAACTGACGACTACAATT 196 583 319 Antisense
ACAATTGTGCCAATGCTGCTTACCC 408 115 338 Antisense
AACTGCGCTACAATCACCAAAGCTC 65 143 370 Antisense
GCTCAATGTAACTCAGTCACCTGGA 37 301 391 Antisense
GAGAACCATCATCGCTACAGACTGC 662 391 414 Antisense
GCATGCGGATTCTGTAACTCTGGTG 656 309 445 Antisense
AATGCAAACCTTCGTCAATCAATAC 264 165 528 Antisense
CTCATGTACCTCCTGGGCTAAGAAC 184 229 648 Antisense
Sequence Source Affymetrix Proprietary Database