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[186044_at]

Description H23L24.3 /REP_DB=WormBase Gene ID /WP=CE23818 /TR=Q9N5L6 /GB=AAF39893.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 16      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_total > 14      4grp_total > 30      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 246.4
(3)
P 		175.6
(0.6)
P 		68.9
(-3)
P 		179.8
(0.7)
P 		178
B 281.1
(3)
P 		161.1
(-0.6)
P 		82.1
(-3)
P 		180.2
(-0)
P 		199
C 256.5
(3)
P 		124.4
(-1.8)
P 		91.1
(-3)
P 		181.8
(0.3)
P 		165
avr 261.3
(3) 		153.7
(-0.6) 		80.7
(-3) 		180.6
(0.3) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 120.1
(3)
P 		90.4
(0.9)
P 		83.9
(0.5)
P 		64.7
(-0.8)
P 		100.6
(1.7)
P 		33.2
(-3)
A 		87
B 115.1
(3)
P 		101.5
(1.7)
P 		111.6
(2.7)
P 		94.6
(1)
P 		53.4
(-3)
P 		83.7
(-0.1)
P 		62
C 105.2
(2.4)
P 		113.4
(3)
P 		52.8
(-1.6)
P 		79.8
(0.4)
P 		35.2
(-3)
P 		68
(-0.5)
P 		78
avr 113.5
(3) 		101.8
(1.6) 		82.8
(-0.5) 		79.7
(-0.9) 		63.1
(-2.8) 		61.6
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 89.7
(3)
P 		38.1
(-1.3)
P 		18
(-3)
P 		26.3
(-2.3)
P 		72
B 89.6
(3)
P 		39.8
(-1.2)
P 		19
(-3)
P 		38.4
(-1.4)
P 		71
C 84.9
(3)
P 		35.8
(-2.4)
P 		31.7
(-2.9)
P 		30.7
(-3)
P 		54
avr 88.1
(3) 		37.9
(-1.6) 		22.9
(-3) 		31.8
(-2.2) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 22.1
(0.5)
P 		32.2
(3)
P 		18.3
(-0.5)
P 		16.4
(-0.9)
P 		30.4
(2.6)
P 		8.1
(-3)
A 		24
B 40
(3)
P 		33.8
(1)
P 		27.1
(-1.2)
P 		25.1
(-1.9)
P 		21.7
(-3)
P 		31.2
(0.1)
P 		18
C 39
(3)
P 		34.6
(2)
P 		13.4
(-2.6)
P 		16
(-2)
P 		11.4
(-3)
P 		22.8
(-0.5)
P 		28
avr 33.7
(3) 		33.5
(2.9) 		19.6
(-2.8) 		19.2
(-3) 		21.2
(-2.2) 		20.7
(-2.4) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186044_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8374
Sequence Type Exemplar sequence
Representative Public ID H23L24.3 NCBI
Target Description H23L24.3 /REP_DB=WormBase Gene ID /WP=CE23818 /TR=Q9N5L6 /GB=AAF39893.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171405(10), NM_171406(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171405 NCBI Caenorhabditis elegans H23L24.3b (H23L24.3) mRNA, complete cds. 10/11 None
NM_171406 NCBI Caenorhabditis elegans H23L24.3a (H23L24.3) mRNA, complete cds. 10/11 None
SNAP00000025372 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8697719:8702634:1 10/11 None
GENEFINDER00000025382 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8692112:8702634:1 10/11 None
H23L24.3b ENSEMBL cdna:known chromosome:CEL140:IV:8697718:8702654:1 gene:H23L24.3 10/11 None
H23L24.3a ENSEMBL cdna:known chromosome:CEL140:IV:8700268:8702634:1 gene:H23L24.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8697724-8702640(+) 100.0 100.0
Public Domain and Genome References
Ensembl H23L24.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM98008 Hypothetical protein H23L24.3b [Caenorhabditis elegans] ref|NP_741471.2| H23L24.3b [Caenorhabditis elegans] 0.0
blast AAL06035 Hypothetical protein H23L24.3a [Caenorhabditis elegans] ref|NP_741472.1| H23L24.3a [Caenorhabditis elegans] 0.0
blast CAE65617 Hypothetical protein CBG10653 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004344 EMBL-EBI Tubulin-tyrosine ligase 2.2E-63
Pfam IPR004344 EMBL-EBI Tubulin-tyrosine ligase 2.2E-63
Pfam IPR004344 EMBL-EBI Tubulin-tyrosine ligase 2.2E-63
Sequence
Target Sequence 
>C. ELEGANS:186044_AT
gaggcggaagccacatttgagtgagattttcccaactcgttatggagctcatagtggaca
tttgctctttcttgacaaagccatgtatatttacatgcagtttgtacaacttcgaagtaa
tgttaacataacgaacgctggtttgaagcaatttgttaggtacttaaacttttcgccact
gaaaaaagattttaaatttaggaagtgcaatttgatagatattataccagttgtgcatgt
ggatgcaaaagtttcagaaataaattattatttcactggagagaaaagaacaaatggaaa
tggtctcccattccacgcctttctaatgtttttgtttttcattgctgaaaagaagtttgt
gttagaaaatgatttattatcaaaagtgcagagacttttatctttttgcgatatgagttt
aagacgttatggagttcgatcagcaaggttaagaagagctgaagttgattcaactattgg
aaatgttgaaatttatatgctaccttctcgtatggcgagaaatcgatctggaacaaatgg
acggaagcaaaacttcaccgatgacaataacaacccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGCGGAAGCCACATTTGAGTGAG 153 405 1647 Antisense
GATTTTCCCAACTCGTTATGGAGCT 218 425 1671 Antisense
AGTGGACATTTGCTCTTTCTTGACA 268 59 1699 Antisense
TAGGTACTTAAACTTTTCGCCACTG 191 655 1803 Antisense
AGATATTATACCAGTTGTGCATGTG 59 67 1863 Antisense
AATGGAAATGGTCTCCCATTCCACG 197 163 1939 Antisense
CCATTCCACGCCTTTCTAATGTTTT 498 269 1954 Antisense
AGTGCAGAGACTTTTATCTTTTTGC 27 61 2031 Antisense
GACGTTATGGAGTTCGATCAGCAAG 183 379 2069 Antisense
TTTATATGCTACCTTCTCGTATGGC 309 673 2138 Antisense
TTCACCGATGACAATAACAACCCGA 93 687 2200 Antisense
Sequence Source Affymetrix Proprietary Database