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[186041_at]

Description T25G12.3 /REP_DB=WormBase Gene ID /WP=CE07540 /TR=Q22784 /GB=AAC69019.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 51      4grp_high_8_8 > 53      6grp_highest_nonorm > 61      6grp_total > 41      4grp_total > 58      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1147
1 2 3 4 range
A 525.4
(0.4)
P 		877.4
(3)
P 		848.4
(2.8)
P 		50.4
(-3)
A 		827
B 448.9
(-0.8)
P 		1179.9
(3)
P 		1071.1
(2.4)
P 		33.3
(-3)
A 		1147
C 463.1
(-0.7)
P 		1040
(3)
P 		925.4
(2.3)
P 		97.9
(-3)
P 		942
avr 479.1
(-0.4) 		1032.4
(3) 		948.3
(2.5) 		60.5
(-3) 		972

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 399
1 2 3 4 5 6 range
A 297.7
(-1.2)
P 		402.7
(0.7)
P 		525.1
(3)
P 		523.4
(3)
P 		392.5
(0.5)
P 		200.8
(-3)
P 		324
B 451
(3)
P 		379.6
(1.6)
P 		439.7
(2.8)
P 		416.3
(2.3)
P 		328.4
(0.5)
P 		152.3
(-3)
P 		299
C 352.9
(0.4)
P 		419.6
(1.4)
P 		524.5
(3)
P 		349.9
(0.4)
P 		334.6
(0.1)
P 		125.9
(-3)
P 		399
avr 367.2
(0.7) 		400.6
(1.3) 		496.4
(3) 		429.9
(1.8) 		351.8
(0.4) 		159.7
(-3) 		337
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 315
1 2 3 4 range
A 191.3
(2.1)
P 		190.5
(2.1)
P 		221.8
(3)
P 		7.4
(-3)
A 		214
B 143.2
(-0.1)
P 		291.4
(3)
P 		248.2
(2.1)
P 		7.1
(-3)
A 		284
C 153.3
(-0.3)
P 		299
(2.5)
P 		322.5
(3)
P 		16.5
(-3)
P 		306
avr 162.6
(0.6) 		260.3
(2.9) 		264.2
(3) 		10.3
(-3) 		254

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 54.9
(-2.6)
P 		143.2
(3)
P 		114.4
(1.2)
P 		132.7
(2.3)
P 		118.4
(1.4)
P 		48.9
(-3)
P 		94
B 156.9
(3)
P 		126.4
(1.2)
P 		106.8
(-0)
P 		110.3
(0.2)
P 		133.4
(1.6)
P 		56.8
(-3)
P 		100
C 130.8
(2.8)
P 		128
(2.6)
P 		133.5
(3)
P 		70.4
(-1.1)
P 		108.3
(1.3)
P 		42.1
(-3)
P 		91
avr 114.2
(1.7) 		132.5
(3) 		118.2
(2) 		104.5
(1) 		120
(2.1) 		49.3
(-3) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186041_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17744
Sequence Type Exemplar sequence
Representative Public ID T25G12.3 NCBI
Target Description T25G12.3 /REP_DB=WormBase Gene ID /WP=CE07540 /TR=Q22784 /GB=AAC69019.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078391(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078391 NCBI Caenorhabditis elegans T25G12.3 (T25G12.3) mRNA, complete cds. 11/11 None
GENEFINDER00000027886 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17231759:17234167:1 10/11 None
SNAP00000027872 ENSEMBL cdna:SNAP chromosome:CEL140:X:17232466:17234627:1 10/11 None
T25G12.3 ENSEMBL cdna:known chromosome:CEL140:X:17233870:17234167:1 gene:T25G12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17233869-17234167(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25G12.3
Entrez Gene 188908 Entrez gene
SwissProt Q22784 EMBL-EBI
WormBase CE07540 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69019 Hypothetical protein T25G12.3 [Caenorhabditis elegans] ref|NP_510792.1| T25G12.3 [Caenorhabditis elegans] 6.0E-21
blast AAC69019 Hypothetical protein T25G12.3 [Caenorhabditis elegans] ref|NP_510792.1| T25G12.3 [Caenorhabditis elegans] 9.0E-17
blast AAC69019 Hypothetical protein T25G12.3 [Caenorhabditis elegans] ref|NP_510792.1| T25G12.3 [Caenorhabditis elegans] 2.0E-13
Sequence
Target Sequence 
>C. ELEGANS:186041_AT
gaatcacgtgtatatgctgggatggggaggcaacggtcttattggcaccacttcagctaa
accgtctattgttgtctcaaccactaccacaacaaccgcacagccaactacagcaattgc
tactactactatcaatactattattagttcaaccgttcgaccaattgtagtcactaagaa
gccacttccagtattgtccaagaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCACGTGTATATGCTGGGATGG 114 327 15 Antisense
GGGAGGCAACGGTCTTATTGGCACC 451 493 39 Antisense
TATTGGCACCACTTCAGCTAAACCG 521 665 54 Antisense
AGCTAAACCGTCTATTGTTGTCTCA 548 77 69 Antisense
TATTGTTGTCTCAACCACTACCACA 403 665 81 Antisense
CACAGCCAACTACAGCAATTGCTAC 494 195 113 Antisense
ACAGCAATTGCTACTACTACTATCA 389 109 124 Antisense
TATTAGTTCAACCGTTCGACCAATT 703 657 156 Antisense
AACCGTTCGACCAATTGTAGTCACT 520 143 165 Antisense
TGTAGTCACTAAGAAGCCACTTCCA 351 559 180 Antisense
GCCACTTCCAGTATTGTCCAAGAAG 513 277 195 Antisense
Sequence Source Affymetrix Proprietary Database