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[186031_at]

Description R01B10.4 /REP_DB=WormBase Gene ID /WP=CE18091 /TR=O61975 /GB=AAC17768.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 73      6grp_total > 64      4grp_total > 55      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 372.2
(2.4)
P 		277.7
(-3)
P 		362.4
(1.8)
P 		382.8
(3)
P 		105
B 435.8
(3)
P 		423.9
(2.3)
P 		347.3
(-2)
P 		329
(-3)
P 		107
C 403.6
(3)
P 		335.8
(-1.6)
P 		348.9
(-0.7)
P 		315
(-3)
P 		89
avr 403.9
(3) 		345.8
(-2.7) 		352.9
(-2) 		342.3
(-3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 418
1 2 3 4 5 6 range
A 539.9
(-1.8)
P 		527.3
(-2.3)
P 		658.6
(3)
P 		635.9
(2.1)
P 		510.4
(-3)
P 		603.6
(0.8)
P 		148
B 523.6
(-1)
P 		483.9
(-1.6)
P 		761.3
(3)
P 		625.8
(0.7)
P 		402.7
(-3)
P 		575.7
(-0.1)
P 		359
C 448.5
(-1.2)
P 		342.9
(-3)
P 		630
(1.8)
P 		701.7
(3)
P 		486.2
(-0.6)
P 		547
(0.4)
P 		359
avr 504
(-1.6) 		451.4
(-3) 		683.3
(3) 		654.5
(2.3) 		466.4
(-2.6) 		575.4
(0.2) 		232
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 135.5
(3)
P 		60.3
(-2.7)
P 		94.7
(-0.1)
P 		56
(-3)
P 		80
B 139
(3)
P 		104.7
(0)
P 		80.5
(-2.1)
P 		70.1
(-3)
P 		69
C 133.6
(3)
P 		96.5
(0.2)
P 		121.6
(2.1)
P 		53.1
(-3)
P 		81
avr 136
(3) 		87.2
(-0.8) 		98.9
(0.1) 		59.7
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 99.5
(-3)
P 		187.5
(3)
P 		143.4
(-0)
P 		161.3
(1.2)
P 		154
(0.7)
P 		147
(0.2)
P 		88
B 182.2
(-0.6)
P 		161.1
(-3)
P 		184.9
(-0.3)
P 		165.8
(-2.5)
P 		163.6
(-2.7)
P 		214.7
(3)
P 		54
C 166.2
(1.7)
P 		104.6
(-3)
P 		160.4
(1.3)
P 		141.1
(-0.2)
P 		157.3
(1)
P 		183.1
(3)
P 		79
avr 149.3
(-3) 		151.1
(-2.7) 		162.9
(-0.5) 		156.1
(-1.7) 		158.3
(-1.3) 		181.6
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186031_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12472
Sequence Type Exemplar sequence
Representative Public ID R01B10.4 NCBI
Target Description R01B10.4 /REP_DB=WormBase Gene ID /WP=CE18091 /TR=O61975 /GB=AAC17768.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072166(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072166 NCBI Caenorhabditis elegans R01B10.4 (R01B10.4) mRNA, complete cds. 11/11 None
GENEFINDER00000013430 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6048433:6050502:-1 11/11 None
R01B10.4 ENSEMBL cdna:known chromosome:CEL140:V:6048433:6050502:-1 gene:R01B10.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013416 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6048432-6050502(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R01B10.4
Entrez Gene 187504 Entrez gene
SwissProt O61975 EMBL-EBI
WormBase CE18091 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC17768 Hypothetical protein R01B10.4 [Caenorhabditis elegans] ref|NP_504567.1| R01B10.4 [Caenorhabditis elegans] 0.0
blast CAE64522 Hypothetical protein CBG09260 [Caenorhabditis briggsae] 1.0E-121
blast EAA11398 ENSANGP00000021025 [Anopheles gambiae str. PEST] ref|XP_315401.2| ENSANGP00000021025 [Anopheles gambiae str. PEST] 9.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR007217 EMBL-EBI Per1-like 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504567.1 6 96-118,131-153,163-180,216-238,258-280,285-307
R01B10.4 6 96-118,131-153,163-180,216-238,258-280,285-307
GENEFINDER00000013430 6 96-118,131-153,163-180,216-238,258-280,285-307
Sequence
Target Sequence 
>C. ELEGANS:186031_AT
ttcacctggatttcttcatcactttttcacatgtttgattgtgatttcactgagaaaatg
gactattttggagcatattcatttgttttattcgctctctacgtctcggtgatattcaca
aaacaactacaatttttgggaagaggtggaccgaaatatatacaaattctgtttgctctc
gtgtatctaaatcattttatgaaaatgatgcaaaacttcgattatggatacaatatgact
tgctgcattgtattctcactaataactacatgcctctacgttcaccatctatattacaga
aaacgaaatctcggatccttgcaagagtcggacattgtactaattcgtctcataatttgg
gcgaatctgtcaactgctttggaaattctggattttactccggttttctggattttcgat
tcgcattcactcttccatctcgccacaattccaatcccaatctggtggtctgacttccta
gctctctattacgatcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCACCTGGATTTCTTCATCACTTT 559 687 436 Antisense
TTCATTTGTTTTATTCGCTCTCTAC 430 689 513 Antisense
GCTCTCTACGTCTCGGTGATATTCA 533 295 529 Antisense
TTCTGTTTGCTCTCGTGTATCTAAA 607 693 602 Antisense
GACTTGCTGCATTGTATTCTCACTA 398 371 672 Antisense
AATAACTACATGCCTCTACGTTCAC 532 171 696 Antisense
TCTGGATTTTACTCCGGTTTTCTGG 176 607 822 Antisense
GATTTTCGATTCGCATTCACTCTTC 604 427 846 Antisense
GCCACAATTCCAATCCCAATCTGGT 429 279 877 Antisense
AATCTGGTGGTCTGACTTCCTAGCT 592 167 894 Antisense
CTTCCTAGCTCTCTATTACGATCTA 502 219 909 Antisense
Sequence Source Affymetrix Proprietary Database