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[186029_at]

Description T28C12.3 /REP_DB=WormBase Gene ID /WP=CE14341 /TR=O16696 /GB=AAB66158.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 28      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 25.7
(-0.6)
A 		59.3
(3)
A 		4
(-3)
A 		40.5
(1)
A 		55
B 23.1
(3)
A 		21
(2.1)
A 		10.4
(-2.2)
A 		8.5
(-3)
A 		15
C 39.9
(0.6)
A 		17.2
(-1.7)
A 		4.7
(-3)
A 		64
(3)
A 		59
avr 29.6
(1.4) 		32.5
(2) 		6.4
(-3) 		37.7
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 52.7
(-0.5)
A 		2.9
(-3)
A 		6.3
(-2.8)
A 		32.5
(-1.5)
A 		92.1
(1.4)
A 		123.9
(3)
P 		121
B 4.2
(-3)
A 		29.6
(-1.7)
A 		23.8
(-2)
A 		5.6
(-2.9)
A 		83.6
(1)
A 		124.3
(3)
P 		120
C 6.5
(-3)
A 		28.7
(-1.8)
A 		16.5
(-2.5)
A 		85.1
(1.3)
A 		46.1
(-0.8)
A 		116.2
(3)
P 		110
avr 21.1
(-2.7) 		20.4
(-2.7) 		15.5
(-3) 		41.1
(-1.6) 		73.9
(0.3) 		121.5
(3) 		106
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 9.4
(1.2)
A 		12.9
(3)
A 		1
(-3)
A 		5.9
(-0.5)
A 		12
B 7.4
(3)
A 		5.2
(0.6)
A 		2.4
(-2.4)
A 		1.8
(-3)
A 		6
C 13.2
(3)
A 		4.9
(-1.3)
A 		1.6
(-3)
A 		10.8
(1.8)
A 		12
avr 10
(3) 		7.7
(1.3) 		1.7
(-3) 		6.2
(0.3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 9.7
(-1.2)
A 		1
(-3)
A 		1.4
(-2.9)
A 		8.3
(-1.5)
A 		27.8
(2.5)
A 		30.2
(3)
P 		29
B 1.5
(-3)
A 		9.9
(-1.9)
A 		5.8
(-2.4)
A 		1.5
(-3)
A 		34
(1.4)
A 		46.3
(3)
P 		45
C 2.4
(-3)
A 		8.7
(-2)
A 		4.2
(-2.7)
A 		17.1
(-0.6)
A 		14.9
(-0.9)
A 		38.9
(3)
P 		37
avr 4.5
(-2.9) 		6.5
(-2.5) 		3.8
(-3) 		9
(-2.1) 		25.6
(0.8) 		38.5
(3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186029_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12370
Sequence Type Exemplar sequence
Representative Public ID T28C12.3 NCBI
Target Description T28C12.3 /REP_DB=WormBase Gene ID /WP=CE14341 /TR=O16696 /GB=AAB66158.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072214(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072214 NCBI Caenorhabditis elegans T28C12.3 (T28C12.3) mRNA, complete cds. 11/11 None
SNAP00000017146 ENSEMBL cdna:SNAP chromosome:CEL140:V:6329897:6331421:1 11/11 None
GENEFINDER00000017153 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6329897:6331421:1 11/11 None
T28C12.3 ENSEMBL cdna:known chromosome:CEL140:V:6329840:6331421:1 gene:T28C12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6329839-6331421(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28C12.3
Entrez Gene 189038 Entrez gene
SwissProt O16696 EMBL-EBI
WormBase CE14341 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66158 Hypothetical protein T28C12.3 [Caenorhabditis elegans] ref|NP_504615.1| T28C12.3 [Caenorhabditis elegans] 0.0
blast NP_504642 T05H4.2 [Caenorhabditis elegans] gb|AAB66009.1| Hypothetical protein T05H4.2 [Caenorhabditis elegans] 1.0E-121
blast CAB07161 Hypothetical protein C06H5.1 [Caenorhabditis elegans] ref|NP_741646.1| C06H5.1 [Caenorhabditis elegans] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.9E-37
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.0E-7
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.9E-37
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.0E-7
Sequence
Target Sequence 
>C. ELEGANS:186029_AT
aagtcggtatctctcgattgtgctcacactgaccaagtcgtttcacttctcaaacacttt
gagcctgaaactctggagagcttacacatcgaaattcttgaatttgatgaggattccgtc
aaagaactggtgattctcgatcaatgggaagaagcaaagactctttcactcactactgtc
gcttatttttcacttatcgtcaagcatctttaccatttcgaatcattgaagttcaacttt
ttgggagaaacttttttaatggatgatatcatcaaatttcgcgatgaaattctgctaaaa
tcgtccgtgttcaaatcacttacttgtgagagtgtggatttcgatgcgagaatgtttgtt
agagtcttcgatccaaactcatctcgtagagactcctctggttcaattgcttataaaact
aaaaaggatacattcatcatcacttatcaaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTCGGTATCTCTCGATTGTGCTC 27 159 811 Antisense
GATTGTGCTCACACTGACCAAGTCG 562 433 826 Antisense
GACCAAGTCGTTTCACTTCTCAAAC 401 383 841 Antisense
AACTCTGGAGAGCTTACACATCGAA 510 141 879 Antisense
TCACTCACTACTGTCGCTTATTTTT 6 627 976 Antisense
TGTCGCTTATTTTTCACTTATCGTC 672 557 987 Antisense
GTCAAGCATCTTTACCATTTCGAAT 3 463 1009 Antisense
ATTCTGCTAAAATCGTCCGTGTTCA 485 9 1099 Antisense
GAGTCTTCGATCCAAACTCATCTCG 455 399 1172 Antisense
AGAGACTCCTCTGGTTCAATTGCTT 514 69 1198 Antisense
TACATTCATCATCACTTATCAACCG 231 645 1239 Antisense
Sequence Source Affymetrix Proprietary Database