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[186022_at]

Description T12A2.15 /REP_DB=WormBase Gene ID /WP=CE18256 /TR=O76835 /GB=AAC24452.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 22      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 70      6grp_total > 22      4grp_total > 52      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 279.1
(0.1)
P 		357.9
(3)
P 		257.3
(-0.7)
P 		195.8
(-3)
P 		162
B 293.4
(1.5)
P 		330.6
(3)
P 		331.5
(3)
P 		182.1
(-3)
P 		149
C 366.4
(3)
P 		325.4
(1.5)
P 		323.3
(1.4)
P 		203.8
(-3)
P 		163
avr 313
(2) 		338
(3) 		304
(1.6) 		193.9
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 291.5
(0.6)
P 		373.5
(3)
P 		268.4
(-0.1)
P 		258.2
(-0.4)
P 		215
(-1.7)
P 		171.6
(-3)
P 		202
B 299.7
(3)
P 		269.7
(1.5)
P 		248.2
(0.4)
P 		209.4
(-1.5)
P 		214.9
(-1.2)
P 		179.8
(-3)
P 		120
C 311.3
(1.6)
P 		319.1
(1.9)
P 		343.8
(3)
P 		319
(1.9)
P 		207.7
(-3)
P 		218.6
(-2.5)
P 		136
avr 300.8
(2.1) 		320.8
(3) 		286.8
(1.4) 		262.2
(0.3) 		212.5
(-2) 		190
(-3) 		131
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 101.6
(3)
P 		77.7
(1)
P 		67.3
(0.2)
P 		28.7
(-3)
P 		73
B 93.6
(3)
P 		81.6
(1.7)
P 		76.8
(1.2)
P 		38.8
(-3)
P 		55
C 121.3
(3)
P 		93.5
(1.1)
P 		112.7
(2.4)
P 		34.4
(-3)
P 		87
avr 105.5
(3) 		84.3
(1.2) 		85.6
(1.3) 		34
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 53.7
(-2.2)
P 		132.8
(3)
P 		58.4
(-1.9)
P 		65.5
(-1.4)
P 		64.9
(-1.5)
P 		41.8
(-3)
P 		91
B 104.3
(3)
P 		89.8
(1.2)
P 		60.3
(-2.4)
P 		55.5
(-3)
P 		87.3
(0.9)
P 		67
(-1.6)
P 		49
C 115.4
(3)
P 		97.4
(0.9)
P 		87.5
(-0.3)
P 		64.1
(-3)
P 		67.2
(-2.6)
P 		73.2
(-1.9)
P 		51
avr 91.1
(1) 		106.7
(3) 		68.7
(-2) 		61.7
(-2.9) 		73.1
(-1.4) 		60.7
(-3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186022_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7411
Sequence Type Exemplar sequence
Representative Public ID T12A2.15 NCBI
Target Description T12A2.15 /REP_DB=WormBase Gene ID /WP=CE18256 /TR=O76835 /GB=AAC24452.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171156(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171156 NCBI Caenorhabditis elegans T12A2.15a (T12A2.15) mRNA, complete cds. 11/11 None
SNAP00000026120 ENSEMBL cdna:SNAP chromosome:CEL140:III:6224557:6229321:-1 11/11 None
GENEFINDER00000026141 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6224557:6236927:-1 11/11 None
T12A2.15a ENSEMBL cdna:known chromosome:CEL140:III:6224557:6229342:-1 gene:T12A2.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175882 2 1 NM_171156 186022_at (11)
NM_171157 185660_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6226767-6229407(-) 100.0 100.0
Public Domain and Genome References
Ensembl T12A2.15
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24452 Hypothetical protein T12A2.15a [Caenorhabditis elegans] ref|NP_741181.1| T12A2.15a [Caenorhabditis elegans] 0.0
blast CAE70749 Hypothetical protein CBG17495 [Caenorhabditis briggsae] 0.0
blast AAC24444 Hypothetical protein T12A2.5 [Caenorhabditis elegans] ref|NP_498357.1| T12A2.5 [Caenorhabditis elegans] 1.0E-153
blast AAC24451 Hypothetical protein T12A2.15b [Caenorhabditis elegans] ref|NP_741182.1| T12A2.15b [Caenorhabditis elegans] 1.0E-121
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 6.6E-30
Pfam IPR000008 EMBL-EBI C2 domain 1.2E-13
Pfam IPR000008 EMBL-EBI C2 domain 2.6E-15
Pfam IPR000008 EMBL-EBI C2 domain 6.6E-30
Pfam IPR000008 EMBL-EBI C2 domain 1.2E-13
Pfam IPR000008 EMBL-EBI C2 domain 2.6E-15
Pfam IPR000008 EMBL-EBI C2 domain 6.6E-30
Pfam IPR000008 EMBL-EBI C2 domain 1.2E-13
Pfam IPR000008 EMBL-EBI C2 domain 2.6E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741181.1 2 5-23,30-47
T12A2.15a 2 5-23,30-47
SNAP00000026120 2 5-23,30-47
GENEFINDER00000026141 3 12-31,632-654,661-678
Sequence
Target Sequence 
>C. ELEGANS:186022_AT
ggagatcttcacatcaaagccacatggatgaatctctccacagaactccgtcatcttgag
aaacaagaatgggaagctgaatggggacaagcagataaaccaattcattcggcactactc
atggtttatattgattctgtagcagaccttccatatccaaagtcaaaacttgagccatct
ccatttgtggaagtcagtttgggaaaggagacacagagaacaccggtcaaagtgaaaacg
gttaatccactgtttcagtctaagttcttgttttttgtgagacatctagaaggacaggaa
ttgaaatttgaggcagtggacgatggtacacgcagatctcttggctctctgaacattcct
cttacgacacttctcaaagaacctaatttggagcagaaccaacagatgcacatgctcaca
ctaggtgttcaccagagtcccatag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGATCTTCACATCAAAGCCACAT 94 519 1255 Antisense
GGATGAATCTCTCCACAGAACTCCG 286 513 1280 Antisense
ATTCGGCACTACTCATGGTTTATAT 254 9 1361 Antisense
ATTCTGTAGCAGACCTTCCATATCC 488 9 1388 Antisense
AAACTTGAGCCATCTCCATTTGTGG 229 127 1420 Antisense
AACGGTTAATCCACTGTTTCAGTCT 35 1 1491 Antisense
GGACGATGGTACACGCAGATCTCTT 621 523 1572 Antisense
CAGATCTCTTGGCTCTCTGAACATT 579 205 1587 Antisense
TCTCTGAACATTCCTCTTACGACAC 505 611 1600 Antisense
AGATGCACATGCTCACACTAGGTGT 432 67 1658 Antisense
CTAGGTGTTCACCAGAGTCCCATAG 359 225 1675 Antisense
Sequence Source Affymetrix Proprietary Database