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[186021_s_at]

Description W03D8.10 /REP_DB=WormBase Gene ID /WP=CE18330 /TR=O45005 /GB=AAK21495.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 04      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 7.1
(3)
A 		4
(-3)
A 		4.7
(-1.6)
A 		4.7
(-1.6)
A 		3
B 7
(3)
A 		3
(-2.6)
A 		4.3
(-0.8)
A 		2.7
(-3)
A 		4
C 2.3
(-3)
A 		6.7
(-0.9)
A 		6.6
(-1)
A 		15.1
(3)
A 		13
avr 5.5
(-1.1) 		4.6
(-3) 		5.2
(-1.8) 		7.5
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 930
1 2 3 4 5 6 range
A 5.1
(-3)
A 		9.3
(-3)
A 		9.4
(-3)
A 		59
(-2.7)
A 		624.1
(1)
P 		933.2
(3)
P 		928
B 3.1
(-3)
A 		7.6
(-3)
A 		3.2
(-3)
A 		63.7
(-2.5)
A 		538.5
(1.2)
P 		770.7
(3)
P 		768
C 8.1
(-3)
A 		4.9
(-3)
A 		4.1
(-3)
A 		51.4
(-2.6)
A 		549.6
(1.5)
P 		726.4
(3)
P 		722
avr 5.4
(-3) 		7.3
(-3) 		5.6
(-3) 		58
(-2.6) 		570.7
(1.2) 		810.1
(3) 		805
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 2.6
(3)
A 		0.9
(-2.4)
A 		1.2
(-1.4)
A 		0.7
(-3)
A 		2
B 2.2
(3)
A 		0.8
(-2.3)
A 		1
(-1.5)
A 		0.6
(-3)
A 		2
C 0.8
(-3)
A 		1.9
(0.9)
A 		2.3
(2.3)
A 		2.5
(3)
A 		2
avr 1.9
(3) 		1.2
(-3) 		1.5
(-0.4) 		1.3
(-2.1) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 287
1 2 3 4 5 6 range
A 0.9
(-3)
A 		3.3
(-2.9)
A 		2
(-3)
A 		15
(-2.6)
A 		188.3
(2)
P 		227.2
(3)
P 		226
B 1.1
(-3)
A 		2.5
(-3)
A 		0.8
(-3)
A 		16.9
(-2.7)
A 		218.8
(1.6)
P 		287.4
(3)
P 		287
C 3
(-3)
A 		1.5
(-3)
A 		1
(-3)
A 		10.3
(-2.8)
A 		177.8
(1.4)
P 		243.2
(3)
P 		242
avr 1.7
(-3) 		2.4
(-3) 		1.3
(-3) 		14.1
(-2.7) 		195
(1.6) 		252.6
(3) 		251
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186021_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.325
Sequence Type Exemplar sequence
Representative Public ID W03D8.10 NCBI
Target Description W03D8.10 /REP_DB=WormBase Gene ID /WP=CE18330 /TR=O45005 /GB=AAK21495.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058643(11), NM_058644(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058643 NCBI Caenorhabditis elegans W03D8.9 (W03D8.9) mRNA, complete cds. 11/11 None
NM_058644 NCBI Caenorhabditis elegans W03D8.10 (W03D8.10) mRNA, complete cds. 11/11 None
SNAP00000016952 ENSEMBL cdna:SNAP chromosome:CEL140:I:2784668:2785797:-1 11/11 None
GENEFINDER00000016972 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2784806:2785797:-1 9/11 None
SNAP00000016953 ENSEMBL cdna:SNAP chromosome:CEL140:I:2786388:2787513:1 11/11 None
GENEFINDER00000016963 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2786565:2787513:1 11/11 None
W03D8.9 ENSEMBL cdna:known chromosome:CEL140:I:2784647:2786069:-1 gene:W03D8.9 11/11 None
W03D8.10 ENSEMBL cdna:known chromosome:CEL140:I:2786136:2787513:1 gene:W03D8.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2786707-2787513(+) 97.55 97.55
chrI:2784667-2785476(-) 95.62 95.62
Public Domain and Genome References
Gene Title

Ensembl W03D8.9
W03D8.10
Entrez Gene 171842 Entrez gene
171843 Entrez gene
SwissProt O45005 EMBL-EBI
O45006 EMBL-EBI
WormBase CE18330 Wormbase
CE26983 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491044 W03D8.9 [Caenorhabditis elegans] gb|AAK21499.1| Hypothetical protein W03D8.9 [Caenorhabditis elegans] 1.0E-104
blast CAA21525 Hypothetical protein Y37D8A.5 [Caenorhabditis elegans] ref|NP_499671.1| Y37D8A.5 [Caenorhabditis elegans] 1.0E-94
blast D87733 protein W03D8.9 [imported] - Caenorhabditis elegans pir||T32940 hypothetical protein W03D8.9 - Caenorhabditis elegans (fragment) 5.0E-89
blast NP_491045 W03D8.10 [Caenorhabditis elegans] gb|AAK21495.1| Hypothetical protein W03D8.10 [Caenorhabditis elegans] 2.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR008574 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF856 1.0E-126
Pfam IPR008574 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF856 1.0E-126
Pfam IPR008574 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF856 1.0E-126
Pfam IPR008574 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF856 1.0E-126
Pfam IPR008574 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF856 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491044.1 6 119-141,148-170,204-226,233-252,257-279,292-314
NP_491045.1 7 59-81,86-108,121-143,148-165,172-191,196-218,231-253
W03D8.9 6 119-141,148-170,204-226,233-252,257-279,292-314
W03D8.10 7 59-81,86-108,121-143,148-165,172-191,196-218,231-253
SNAP00000016952 6 119-141,148-170,204-226,233-252,257-279,292-314
GENEFINDER00000016972 5 119-141,148-170,204-226,233-252,257-279
SNAP00000016953 6 90-112,119-141,175-197,204-223,228-250,263-285
GENEFINDER00000016963 6 59-81,88-110,144-166,173-192,197-219,232-254
Sequence
Target Sequence 
>C. ELEGANS:186021_S_AT
ttcttctcggagccgggattgccagcattattggtggatatgtgctctcgccaatctttg
gaatcttcattggaagaggtggtgctgcaattctcgcctctcttgttctcccaggagccg
gtgcatattatttgaatgctgaggatggctcaacttctgccacaagatttcaattgttgg
ttctggccttggctcaaggaattttgatgggacactcaatctcgtacacttacttgggtg
gccaaccacttggattcatcactccattggtcattgcattcgcatatccattgattgccg
gacaagttggtacagctcgtgctccattgctgggcggtgcaattggggctgctctcggtg
ttcagcttgtgtttggaatcgtctctggaagcctctcattctcttatttcctcttgagtg
cactttacagtgttgcttctggtgcacttcttcaaattgccaacaagaacatgtcgccac
caaacagacaccatatgtatcaaatcctcttggtctcctcgttcctcttctcgaaggctt
tggtctatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTTCTCGGAGCCGGGATTGCCAG 324 689 206 Antisense
TTGGTGGATATGTGCTCTCGCCAAT 244 707 236 Antisense
AAGAGGTGGTGCTGCAATTCTCGCC 669 149 279 Antisense
GCTCAACTTCTGCCACAAGATTTCA 670 301 353 Antisense
ACACTCAATCTCGTACACTTACTTG 118 111 417 Antisense
GGGTGGCCAACCACTTGGATTCATC 247 497 441 Antisense
TGGATTCATCACTCCATTGGTCATT 336 551 456 Antisense
TCAGCTTGTGTTTGGAATCGTCTCT 603 623 567 Antisense
TTGCCAACAAGAACATGTCGCCACC 687 699 662 Antisense
ATGTATCAAATCCTCTTGGTCTCCT 615 47 700 Antisense
CTCTTCTCGAAGGCTTTGGTCTATG 10 231 730 Antisense
Sequence Source Affymetrix Proprietary Database