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[186010_at]

Description C32E8.11 /REP_DB=WormBase Gene ID /WP=CE08535 /TR=P91133 /GB=AAB42328.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 70      6grp_total > 17      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 294
1 2 3 4 range
A 459.2
(3)
P 		202.2
(-2.3)
P 		165.6
(-3)
P 		267.2
(-0.9)
P 		294
B 412.7
(3)
P 		229.1
(-2.4)
P 		209.8
(-3)
P 		213.6
(-2.9)
P 		203
C 354.8
(3)
P 		213.6
(-3)
P 		237
(-2)
P 		263.2
(-0.9)
P 		141
avr 408.9
(3) 		215
(-2.7) 		204.1
(-3) 		248
(-1.7) 		205

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 359
1 2 3 4 5 6 range
A 223.4
(-2.1)
P 		322.2
(2.4)
P 		202.8
(-3)
P 		257.2
(-0.5)
P 		228.4
(-1.8)
P 		334.7
(3)
P 		132
B 326.7
(3)
P 		314.2
(2.2)
P 		233.7
(-3)
P 		299
(1.2)
P 		234.8
(-2.9)
P 		263.7
(-1.1)
P 		93
C 554
(3)
P 		324.2
(-0.8)
P 		312.6
(-1)
P 		195.1
(-3)
P 		294.3
(-1.3)
P 		197.1
(-3)
P 		359
avr 368
(3) 		320.2
(0.6) 		249.7
(-3) 		250.4
(-3) 		252.5
(-2.9) 		265.2
(-2.2) 		118
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 167.2
(3)
P 		43.9
(-2.8)
P 		43.3
(-2.8)
P 		39.1
(-3)
P 		128
B 131.6
(3)
P 		56.6
(-2.2)
P 		48.6
(-2.8)
P 		45.5
(-3)
P 		86
C 117.4
(3)
P 		61.4
(-1.6)
P 		82.6
(0.1)
P 		44.4
(-3)
P 		73
avr 138.7
(3) 		54
(-2.3) 		58.2
(-2) 		43
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 41.2
(-3)
P 		114.6
(3)
P 		44.2
(-2.8)
P 		65.2
(-1)
P 		68.9
(-0.7)
P 		81.5
(0.3)
P 		73
B 113.7
(3)
P 		104.6
(2)
P 		56.7
(-3)
P 		79.2
(-0.6)
P 		95.4
(1.1)
P 		98.3
(1.4)
P 		57
C 205.4
(3)
P 		98.9
(-0.8)
P 		79.6
(-1.5)
P 		39.2
(-3)
P 		95.2
(-1)
P 		66
(-2)
P 		166
avr 120.1
(3) 		106
(1.6) 		60.2
(-3) 		61.2
(-2.9) 		86.5
(-0.4) 		81.9
(-0.8) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186010_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.287
Sequence Type Exemplar sequence
Representative Public ID C32E8.11 NCBI
Target Description C32E8.11 /REP_DB=WormBase Gene ID /WP=CE08535 /TR=P91133 /GB=AAB42328.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058830(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058830 NCBI Caenorhabditis elegans C32E8.11 (C32E8.11) mRNA, complete cds. 11/11 None
C32E8.11 ENSEMBL cdna:known chromosome:CEL140:I:3807475:3816507:-1 gene:C32E8.11 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3807900-3816507(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32E8.11
Entrez Gene 171953 Entrez gene
SwissProt P91133 EMBL-EBI
WormBase CE08535 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590479_AT similar to ubiquitin protein ligase E3 component n-recognin 2 cfa
CANINE:1585141_AT similar to ubiquitin protein ligase E3 component n-recognin 2 cfa
CANINE_2:CFA.12775.1.A1_S_AT similar to ubiquitin protein ligase E3 component n-recognin 2 cfa
CANINE_2:CFAAFFX.3466.1.S1_S_AT similar to ubiquitin protein ligase E3 component n-recognin 2 cfa
CANINE_2:CFAAFFX.3476.1.S1_S_AT similar to ubiquitin protein ligase E3 component n-recognin 2 cfa
DROSOPHILA_2:1635376_AT dm
DROSGENOME1:154592_AT dm
HU35KSUBA:HUM_ALU_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HU35KSUBB:HUM_ALU_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HU35KSUBC:HUM_ALU_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HU35KSUBD:HUM_ALU_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HUGENEFL:HUM_ALU_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HC-G110:HUM_ALU_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HU35KSUBA:AB002347_AT ubiquitin protein ligase E3 component n-recognin 2 hs
U133_X3P:HS.15303.0.S2_3P_AT ubiquitin protein ligase E3 component n-recognin 2 hs
U133_X3P:HS.15303.0.S1_3P_A_AT ubiquitin protein ligase E3 component n-recognin 2 hs
U133_X3P:1569140_3P_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133_PLUS_2:1569140_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133A_2:212756_S_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133_PLUS_2:212756_S_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133A:212756_S_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133A_2:212760_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133_PLUS_2:212760_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133A:212760_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U95AV2:39797_AT ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133_PLUS_2:215558_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133A_2:215558_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U133A:215558_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
U133_X3P:HS.274277.0.S1_3P_X_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
U133_X3P:215558_3P_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U95C:54034_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U95E:67979_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U95D:75204_R_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U95D:82383_R_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
HG-U95D:90778_AT Ubiquitin protein ligase E3 component n-recognin 2 hs
MG-U74BV2:111993_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MG-U74BV2:111342_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOE430A:1427163_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOUSE430A_2:1427163_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOUSE430_2:1427163_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOE430B:1429515_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOUSE430_2:1429515_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOE430B:1429516_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOUSE430_2:1429516_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOE430B:1453878_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MOUSE430_2:1453878_AT ubiquitin protein ligase E3 component n-recognin 2 mm
MU19KSUBA:TC17136_AT Ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), mRNA mm
MU19KSUBC:TC36982_AT Ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), mRNA mm
MU19KSUBC:TC41199_AT Ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), mRNA mm
RG-U34C:RC_AI171738_AT similar to Ubiquitin protein ligase E3 component n-recognin 2 rn
RAE230A:1374492_AT Similar to Ubiquitin protein ligase E3 component n-recognin 2 rn
RAT230_2:1374492_AT Similar to Ubiquitin protein ligase E3 component n-recognin 2 rn
RG-U34C:RC_AI102666_AT Similar to Ubiquitin protein ligase E3 component n-recognin 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42328 Hypothetical protein C32E8.11 [Caenorhabditis elegans] ref|NP_491231.1| C32E8.11 [Caenorhabditis elegans] sp|P91133|UBR1_CAEEL Ubiquitin-protein ligase E3 component N-recognin (Ubiquitin-protein ligase E3-alpha) 0.0
blast CAE66670 Hypothetical protein CBG12009 [Caenorhabditis briggsae] 0.0
blast AAL32101 ubiquitin ligase E3 alpha-II [Homo sapiens] ref|NP_056070.1| ubiquitin protein ligase E3 component n-recognin 2 [Homo sapiens] emb|CAI14738.1| ubiquitin protein ligase E3 component n-recognin 2 [Homo sapiens] emb|CAI19920.1| ubiquitin protein ligase E3 component n-recognin 2 [Homo sapiens] emb|CAI20399.1| ubiquitin protein ligase E3 component n-recognin 2 [Homo sapiens] sp|Q8IWV8|UBR2_HUMAN Ubiquitin-protein ligase E3 component N-recognin-2 (Ubiquitin-protein ligase E3-alpha-2) (Ubiquitin-protein ligase E3-alpha-II) 1.0E-100
Protein Domains
Database ID Description E-Value
Pfam IPR003126 EMBL-EBI Zn-finger (putative), N-recognin 1.0E-37
Sequence
Target Sequence 
>C. ELEGANS:186010_AT
ttattccgctatctgggtttaccgcataagattgaagaacaaatcagtggaagtatgctc
gaaaagttgttcacaatgtggtcatcatcgattccaagtgatcaagctcttcgacaggat
cttgttgttcaaccagttcgaccgaatcttcttgttgaattgccagagaagtatagtcaa
ttaataaatcaagttgccacattcaaatgtccaacgataccgattgaagagtcaacgtca
aatgttccgacactatgtctagtgtgcggaactatcctctgctcacaggcatattgctgt
cagaaaattatcaacaaacaatcatatggagcatgtcgttatcatatgtcacagtgttcc
ggatcagtgggaatgtttctacgagttcgagactgtagccttgtattgatgacgacacga
aaacgcggttgcttccgtccagcaccatatgtcgacgaattcggagaggttgatcaagga
ttccgacgtggaaatccccttcacctgaaccctgaactctatcagaagctgaagtctttg
tggcttcagcagggtatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATTCCGCTATCTGGGTTTACCGC 450 673 5170 Antisense
GATTCCAAGTGATCAAGCTCTTCGA 613 433 5259 Antisense
GCTCTTCGACAGGATCTTGTTGTTC 304 301 5275 Antisense
GTTGTTCAACCAGTTCGACCGAATC 77 437 5293 Antisense
ACTATCCTCTGCTCACAGGCATATT 161 103 5440 Antisense
GTCACAGTGTTCCGGATCAGTGGGA 110 463 5517 Antisense
GAGTTCGAGACTGTAGCCTTGTATT 701 395 5552 Antisense
CGACACGAAAACGCGGTTGCTTCCG 591 223 5582 Antisense
TTCCGTCCAGCACCATATGTCGACG 519 699 5602 Antisense
TCACCTGAACCCTGAACTCTATCAG 158 627 5670 Antisense
GTCTTTGTGGCTTCAGCAGGGTATC 294 469 5703 Antisense
Sequence Source Affymetrix Proprietary Database