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[186009_at]

Description H34I24.2 /REP_DB=WormBase Gene ID /WP=CE26681 /TR=O76672 /GB=AAK18967.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_highest_8_8 > 33      4grp_high_8_8 > 59      6grp_highest_nonorm > 45      6grp_total > 62      4grp_total > 61      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 867
1 2 3 4 range
A 2044.9
(1.7)
P 		2071.2
(2)
P 		2159.5
(3)
P 		1621.8
(-3)
P 		538
B 2322.9
(3)
P 		1933.9
(0.2)
P 		2149.3
(1.7)
P 		1495.7
(-3)
P 		827
C 2043.8
(2.8)
P 		2005.5
(2.4)
P 		2062.9
(3)
P 		1455.9
(-3)
P 		607
avr 2137.2
(3) 		2003.5
(1.7) 		2123.9
(2.9) 		1524.5
(-3) 		613

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1465
1 2 3 4 5 6 range
A 1991.6
(-2.2)
P 		2123.8
(-1.4)
P 		2854.8
(2.9)
P 		2873.1
(3)
P 		2123.7
(-1.4)
P 		1856.7
(-3)
P 		1016
B 1624.8
(-2.5)
P 		1749.2
(-1.8)
P 		2242.5
(1.1)
P 		2567.9
(3)
P 		1632.8
(-2.5)
P 		1539.6
(-3)
P 		1028
C 1896.9
(-1.6)
P 		1749.3
(-2.2)
P 		3004.4
(3)
P 		1934.8
(-1.4)
P 		2566.4
(1.2)
P 		1545.2
(-3)
P 		1459
avr 1837.8
(-1.9) 		1874.1
(-1.7) 		2700.6
(3) 		2458.6
(1.6) 		2107.6
(-0.4) 		1647.2
(-3) 		1053
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 507
1 2 3 4 range
A 744.5
(3)
P 		449.6
(-0.5)
P 		564.5
(0.9)
P 		237.4
(-3)
P 		507
B 740.8
(3)
P 		477.6
(-0.7)
P 		498
(-0.5)
P 		318.7
(-3)
P 		422
C 676.6
(2.5)
P 		576.6
(1.2)
P 		719
(3)
P 		245.5
(-3)
P 		474
avr 720.6
(3) 		501.3
(0.1) 		593.8
(1.3) 		267.2
(-3) 		453

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 463
1 2 3 4 5 6 range
A 367.1
(-3)
P 		755.3
(3)
P 		621.7
(0.9)
P 		728.7
(2.6)
P 		640.8
(1.2)
P 		452
(-1.7)
P 		388
B 565.3
(-2.1)
P 		582.3
(-1.3)
P 		544.6
(-3)
P 		680.1
(3)
P 		663.4
(2.3)
P 		574.1
(-1.7)
P 		136
C 703.1
(1.3)
P 		533.8
(-1)
P 		764.7
(2.1)
P 		389.1
(-3)
P 		830.5
(3)
P 		517.3
(-1.3)
P 		441
avr 545.2
(-2.1) 		623.8
(0.3) 		643.7
(0.9) 		599.3
(-0.4) 		711.6
(3) 		514.5
(-3) 		197
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186009_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7654
Sequence Type Exemplar sequence
Representative Public ID H34I24.2 NCBI
Target Description H34I24.2 /REP_DB=WormBase Gene ID /WP=CE26681 /TR=O76672 /GB=AAK18967.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065227(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065227 NCBI Caenorhabditis elegans H34I24.2 (H34I24.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000044594 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2842161:2845063:1 11/11 None
SNAP00000044590 ENSEMBL cdna:SNAP chromosome:CEL140:III:2842419:2848543:1 11/11 None
H34I24.2 ENSEMBL cdna:known chromosome:CEL140:III:2842034:2845187:1 gene:H34I24.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2843228-2845063(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H34I24.2
Entrez Gene 175401 Entrez gene
SwissProt O76672 EMBL-EBI
WormBase CE26681 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18967 Hypothetical protein H34I24.2 [Caenorhabditis elegans] ref|NP_497628.1| H34I24.2 [Caenorhabditis elegans] 0.0
blast AAK18966 Hypothetical protein H34I24.1 [Caenorhabditis elegans] ref|NP_497629.1| H34I24.1 [Caenorhabditis elegans] 1.0E-119
blast CAE57498 Hypothetical protein CBG00470 [Caenorhabditis briggsae] 2.0E-79
blast CAE57498 Hypothetical protein CBG00470 [Caenorhabditis briggsae] 6.0E-78
blast CAE57498 Hypothetical protein CBG00470 [Caenorhabditis briggsae] 4.0E-70
Sequence
Target Sequence 
>C. ELEGANS:186009_AT
ttgcacaggaagcgggccatgccaattggatagtctcaacagaccatactgcacaaattg
cccgggagatatggacggatacactcagtgccagcaagttgcctgctcagccgctggaac
taacttcaccattggcacaaataatagaggagtttggtactcccctggctacaactccaa
caatacaaactcctcagttgttccagccaactctaactgtatctataatctaactggata
ttaccaagttaaccaaaccagtggggctcctgaactgaatctaaactgtctggcgtcttc
aaacgttgagttctattttttgaattcggatggttttaaaacgccactcacaagtggaag
ttctgctagagtgattaataacgcgttgggcaagatgaaaactggtggacagattgttat
aacatcgacaagcttggacgcctcttattgtgtgcttccgttggctctgcaaccagctac
aacttcgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCACAGGAAGCGGGCCATGCCAA 629 701 243 Antisense
GACCATACTGCACAAATTGCCCGGG 510 383 284 Antisense
TCAGTGCCAGCAAGTTGCCTGCTCA 273 623 327 Antisense
CAGCCGCTGGAACTAACTTCACCAT 243 203 350 Antisense
ACAACTCCAACAATACAAACTCCTC 686 111 413 Antisense
GTTCCAGCCAACTCTAACTGTATCT 463 439 442 Antisense
GTTAACCAAACCAGTGGGGCTCCTG 6 1 490 Antisense
GGGGCTCCTGAACTGAATCTAAACT 72 499 505 Antisense
AATCTAAACTGTCTGGCGTCTTCAA 368 169 520 Antisense
TAACATCGACAAGCTTGGACGCCTC 381 639 662 Antisense
TCTGCAACCAGCTACAACTTCGAAA 265 609 708 Antisense
Sequence Source Affymetrix Proprietary Database