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[185997_s_at]

Description H42K12.2 /REP_DB=WormBase Gene ID /WP=CE27387 /TR=O17348 /GB=AAK71372.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 64      4grp_total > 12      6grp_cgc > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 28.1
(-1.5)
A 		52.3
(0.7)
A 		11.5
(-3)
A 		77
(3)
A 		66
B 36.3
(-1.3)
A 		28.5
(-2.9)
A 		57
(3)
A 		27.8
(-3)
A 		29
C 59
(3)
A 		31.2
(-3)
A 		34.2
(-2.4)
A 		46.3
(0.3)
A 		28
avr 41.1
(-0.4) 		37.3
(-1.9) 		34.2
(-3) 		50.4
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 30.3
(-0.2)
A 		12
(-2.5)
A 		56.6
(3)
A 		7.5
(-3)
A 		27.1
(-0.6)
A 		37.5
(0.7)
A 		49
B 5.5
(-3)
A 		22.4
(0.3)
A 		9.9
(-2.1)
A 		34.9
(2.8)
A 		27.9
(1.4)
A 		36
(3)
A 		31
C 53.4
(2.8)
A 		13.3
(-3)
A 		54.5
(3)
A 		38
(0.6)
A 		50.1
(2.4)
A 		41.4
(1.1)
A 		41
avr 29.7
(0.4) 		15.9
(-3) 		40.3
(3) 		26.8
(-0.3) 		35
(1.7) 		38.3
(2.5) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 10.2
(2.2)
A 		11.3
(3)
A 		3
(-3)
A 		11.3
(3)
A 		8
B 11.6
(1.7)
A 		7
(-2.1)
A 		13.2
(3)
A 		5.9
(-3)
A 		7
C 19.5
(3)
A 		9
(-2.4)
A 		11.9
(-0.9)
A 		7.8
(-3)
A 		12
avr 13.8
(3) 		9.1
(-2.1) 		9.4
(-1.8) 		8.3
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 5.6
(-0.9)
A 		4.3
(-1.6)
A 		12.3
(3)
A 		1.9
(-3)
A 		8.2
(0.6)
A 		9.1
(1.2)
A 		10
B 1.9
(-3)
A 		7.4
(-0.1)
A 		2.4
(-2.7)
A 		9.3
(0.9)
A 		11.3
(1.9)
A 		13.4
(3)
A 		12
C 19.8
(3)
A 		4.1
(-3)
A 		13.9
(0.7)
A 		7.6
(-1.7)
A 		16.2
(1.6)
A 		13.9
(0.7)
A 		16
avr 9.1
(0.4) 		5.3
(-3) 		9.5
(0.7) 		6.3
(-2.1) 		11.9
(2.8) 		12.1
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185997_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18562
Sequence Type Exemplar sequence
Representative Public ID H42K12.2 NCBI
Target Description H42K12.2 /REP_DB=WormBase Gene ID /WP=CE27387 /TR=O17348 /GB=AAK71372.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029572(11), NM_001029571(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029572 NCBI Caenorhabditis elegans PDK-class protein kinase family member (pdk-1) (pdk-1) mRNA, complete cds. 11/11 None
NM_001029571 NCBI Caenorhabditis elegans PDK-class protein kinase family member (pdk-1) (pdk-1) mRNA, complete cds. 11/11 None
SNAP00000010279 ENSEMBL cdna:SNAP chromosome:CEL140:X:1321358:1322416:1 10/11 None
H42K12.1b ENSEMBL cdna:known chromosome:CEL140:X:1321348:1327980:1 gene:H42K12.1 11/11 A 
H42K12.1a ENSEMBL cdna:known chromosome:CEL140:X:1321348:1327980:1 gene:H42K12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1318860-1322416(+) 96.69 100.0
Public Domain and Genome References
Ensembl H42K12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK82905 Pdk-class protein kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024743.1| PDK-class protein kinase family member (pdk-1) [Caenorhabditis elegans] gb|AAD42307.1| phophoinositide-dependent protein kinase 1a [Caenorhabditis elegans] 0.0
blast AAK82904 Pdk-class protein kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024742.1| PDK-class protein kinase family member (pdk-1) [Caenorhabditis elegans] gb|AAD42308.1| phophoinositide-dependent protein kinase 1b [Caenorhabditis elegans] 0.0
blast CAE63571 Hypothetical protein CBG08059 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-16
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-16
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 6.0E-65
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 1.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.2E-68
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-68
Sequence
Target Sequence 
>C. ELEGANS:185997_S_AT
ccatctcgatggaggatctcacaccaactaacacgtcgctcgacaccacaactactaaca
atgacacgacatcggatcgtgaagcggcgccaacgacgctcaacttaacaccaacagcaa
gtgaatcggagaacagcttatccccagtcaccgccgaagatctcatagctaaaagcatta
aagaaggatgtccgaagagaacttccaacgacttcatgtttcttcagagtatgggcgaag
gagcctacagccaggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATCTCGATGGAGGATCTCACACC 95 269 665 Antisense
TGGAGGATCTCACACCAACTAACAC 19 551 674 Antisense
CTCGACACCACAACTACTAACAATG 264 233 703 Antisense
ACTAACAATGACACGACATCGGATC 620 99 718 Antisense
CGACATCGGATCGTGAAGCGGCGCC 442 251 731 Antisense
GACGCTCAACTTAACACCAACAGCA 127 381 759 Antisense
ATCGGAGAACAGCTTATCCCCAGTC 97 35 789 Antisense
TCACCGCCGAAGATCTCATAGCTAA 50 627 812 Antisense
GTCCGAAGAGAACTTCCAACGACTT 443 475 854 Antisense
CCAACGACTTCATGTTTCTTCAGAG 64 277 869 Antisense
TGGGCGAAGGAGCCTACAGCCAGGT 197 543 896 Antisense
Sequence Source Affymetrix Proprietary Database