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[185984_at]

Description F57C12.1 /REP_DB=WormBase Gene ID /WP=CE04671 /TR=Q20942 /GB=AAA83298.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 80      6grp_total > 72      4grp_total > 57      6grp_cgc > 74      6grp_high1_sub > 02_03_04_05      6grp_cgc_sub > 02_03_04_05      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 907
1 2 3 4 range
A 53.8
(-2.9)
A 		659.4
(2.1)
P 		770.8
(3)
P 		37.3
(-3)
A 		734
B 9.1
(-3)
A 		674
(2)
P 		809.6
(3)
P 		85
(-2.4)
A 		801
C 75
(-3)
A 		650.8
(1.1)
P 		916.4
(3)
P 		125.3
(-2.6)
A 		841
avr 46
(-3) 		661.4
(1.7) 		832.3
(3) 		82.5
(-2.7) 		786

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 234
(-3)
A 		476.5
(3)
P 		344.5
(-0.3)
P 		439.9
(2.1)
P 		376.4
(0.5)
P 		244.2
(-2.7)
P 		243
B 228.9
(-3)
P 		327.7
(0.8)
P 		290.1
(-0.6)
P 		383.3
(3)
P 		379.7
(2.9)
P 		247.4
(-2.3)
P 		154
C 236
(-3)
A 		342.4
(-0.2)
P 		463.6
(3)
P 		334.8
(-0.4)
P 		429.9
(2.1)
P 		301.7
(-1.3)
P 		228
avr 233
(-3) 		382.2
(2.5) 		366.1
(1.9) 		386
(2.7) 		395.3
(3) 		264.4
(-1.8) 		162
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 19.6
(-2.6)
A 		143.1
(1.2)
P 		201.5
(3)
P 		5.5
(-3)
A 		196
B 2.9
(-3)
A 		166.5
(2.3)
P 		187.6
(3)
P 		18.1
(-2.5)
A 		185
C 24.8
(-2.9)
A 		187.1
(0.3)
P 		319.4
(3)
P 		21.1
(-3)
A 		298
avr 15.8
(-3) 		165.6
(1.1) 		236.2
(3) 		14.9
(-3) 		221

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 43.1
(-3)
A 		169.5
(3)
P 		75
(-1.5)
P 		111.6
(0.3)
P 		113.6
(0.3)
P 		59.5
(-2.2)
P 		126
B 79.6
(-2.3)
P 		109.1
(-0.2)
P 		70.4
(-3)
P 		101.5
(-0.8)
P 		154.3
(3)
P 		92.2
(-1.4)
P 		84
C 87.5
(-1.3)
A 		104.5
(0.1)
P 		118
(1.2)
P 		67.3
(-3)
P 		139.1
(3)
P 		101
(-0.2)
P 		72
avr 70.1
(-3) 		127.7
(2.3) 		87.8
(-1.4) 		93.5
(-0.9) 		135.7
(3) 		84.2
(-1.7) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185984_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18266
Sequence Type Exemplar sequence
Representative Public ID F57C12.1 NCBI
Target Description F57C12.1 /REP_DB=WormBase Gene ID /WP=CE04671 /TR=Q20942 /GB=AAA83298.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075719(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075719 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-38) (nas-38) mRNA, complete cds. 11/11 None
GENEFINDER00000008061 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:558191:563630:1 11/11 None
SNAP00000008050 ENSEMBL cdna:SNAP chromosome:CEL140:X:559500:563630:1 11/11 None
F57C12.1 ENSEMBL cdna:known chromosome:CEL140:X:558142:563658:1 gene:F57C12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:558190-563630(+) 97.71 97.71
Public Domain and Genome References
Gene Title
Gene Symbol nas-38
Ensembl F57C12.1
Entrez Gene 180407 Entrez gene
SwissProt Q20942 EMBL-EBI
WormBase CE31188 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1623942_AT dm
DROSGENOME1:149932_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83298 Nematode astacin protease protein 38 [Caenorhabditis elegans] ref|NP_508120.2| Nematode AStacin protease family member (nas-38) [Caenorhabditis elegans] sp|Q20942|NAS38_CAEEL Zinc metalloproteinase nas-38 precursor (Nematode astacin 38) 0.0
blast CAE63649 Hypothetical protein CBG08147 [Caenorhabditis briggsae] 0.0
blast CAA96596 Hypothetical protein C26C6.3 [Caenorhabditis elegans] ref|NP_492109.2| Nematode AStacin protease family member (nas-36) [Caenorhabditis elegans] 3.0E-70
blast CAA96596 Hypothetical protein C26C6.3 [Caenorhabditis elegans] ref|NP_492109.2| Nematode AStacin protease family member (nas-36) [Caenorhabditis elegans] 4.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.9E-104
Pfam IPR000859 EMBL-EBI CUB 4.9E-13
Pfam IPR000859 EMBL-EBI CUB 8.3E-16
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 7.9E-92
Sequence
Target Sequence 
>C. ELEGANS:185984_AT
ctaacttcctcgtcaactacaacggagtctaccactgtatcttctacgacacaatcaact
acctggttgccaactgaaccatcattcgctaccggcgaaacggaaatcacaactgcaagc
cccacaatcacgttattcccaagtttatcaactatacttcctcctatcaattcgttagct
ggagttctaccatctacacaagctccagatattatcaatagtgtattagaatgcggctgt
ggagcttggtctgaatggcaaggtgaatgctcccagcagtgtggcggatgcggtcatcga
ctgaggaagcgagagtgcaaaaaagaagcttgcagaaaagaagaaaaacgcccatgcaac
ttctcagcatgccccgacggcactaacttcctaatcaacaatgctgaattccatattctt
tggagagggtgttgtgttgggttgttcagatccggagatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTAACTTCCTCGTCAACTACAACGG 250 227 1741 Antisense
AGTCTACCACTGTATCTTCTACGAC 227 61 1766 Antisense
TGCCAACTGAACCATCATTCGCTAC 482 587 1808 Antisense
AATCACGTTATTCCCAAGTTTATCA 370 171 1866 Antisense
TACTTCCTCCTATCAATTCGTTAGC 305 567 1895 Antisense
GAGTTCTACCATCTACACAAGCTCC 115 399 1922 Antisense
GGCTGTGGAGCTTGGTCTGAATGGC 34 539 1975 Antisense
TGAATGCTCCCAGCAGTGTGGCGGA 17 577 2004 Antisense
TGTGGCGGATGCGGTCATCGACTGA 154 555 2020 Antisense
AGAAAAACGCCCATGCAACTTCTCA 571 71 2082 Antisense
GTTGGGTTGTTCAGATCCGGAGATC 374 437 2176 Antisense
Sequence Source Affymetrix Proprietary Database