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[185969_s_at]

Description C32F10.4 /REP_DB=WormBase Gene ID /WP=CE08541 /TR=O01681 /GB=AAC24267.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 93      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 46      6grp_total > 80      4grp_total > 03      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19368
1 2 3 4 range
A 2574.6
(-2.5)
P 		1119.7
(-2.9)
P 		905.2
(-3)
P 		20155.7
(3)
P 		19251
B 2807.1
(-2.3)
P 		850.4
(-3)
P 		925.6
(-3)
P 		17391.8
(3)
P 		16541
C 2748
(-2.4)
P 		788
(-3)
P 		1004.4
(-2.9)
P 		19689.7
(3)
P 		18902
avr 2709.9
(-2.4) 		919.4
(-3) 		945.1
(-3) 		19079.1
(3) 		18160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1011
1 2 3 4 5 6 range
A 2743.6
(-3)
P 		2763.5
(-2.8)
P 		2838.2
(-2.1)
P 		3391.9
(3)
P 		3007.1
(-0.6)
P 		3014.7
(-0.5)
P 		648
B 3174.5
(0.4)
P 		2903.3
(-3)
P 		2972.6
(-2.1)
P 		3375.9
(3)
P 		3328.2
(2.4)
P 		3176.2
(0.5)
P 		473
C 2600.8
(-3)
P 		2755.2
(-2.1)
P 		2677
(-2.5)
P 		3611.5
(3)
P 		2858.2
(-1.5)
P 		3329.6
(1.3)
P 		1011
avr 2839.6
(-2.7) 		2807.3
(-3) 		2829.3
(-2.8) 		3459.8
(3) 		3064.5
(-0.6) 		3173.5
(0.4) 		653
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3496
1 2 3 4 range
A 937.4
(-1.5)
P 		243.1
(-3)
P 		236.6
(-3)
P 		2950.4
(3)
P 		2714
B 895.1
(-1.8)
P 		210
(-3)
P 		214.5
(-3)
P 		3706.2
(3)
P 		3496
C 909.7
(-1.7)
P 		226.6
(-3)
P 		350.1
(-2.8)
P 		3320
(3)
P 		3093
avr 914.1
(-1.7) 		226.6
(-3) 		267.1
(-2.9) 		3325.5
(3) 		3099

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 846
1 2 3 4 5 6 range
A 505.8
(-3)
P 		982.8
(3)
P 		618.1
(-1.6)
P 		860.3
(1.5)
P 		907.4
(2.1)
P 		734
(-0.1)
P 		477
B 1104.6
(0.6)
P 		966.4
(-0.7)
P 		721.8
(-3)
P 		894.2
(-1.4)
P 		1352.2
(3)
P 		1184.4
(1.4)
P 		630
C 964
(0.9)
P 		840.7
(-0.8)
P 		681.4
(-3)
P 		726.3
(-2.4)
P 		924.9
(0.4)
P 		1114.6
(3)
P 		433
avr 858.1
(-0.1) 		930
(1) 		673.8
(-3) 		826.9
(-0.6) 		1061.5
(3) 		1011
(2.2) 		388
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185969_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2081
Sequence Type Exemplar sequence
Representative Public ID C32F10.4 NCBI
Target Description C32F10.4 /REP_DB=WormBase Gene ID /WP=CE08541 /TR=O01681 /GB=AAC24267.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059286(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059286 NCBI Caenorhabditis elegans C32F10.4 (C32F10.4) mRNA, complete cds. 11/11 None
SNAP00000021234 ENSEMBL cdna:SNAP chromosome:CEL140:I:5816634:5818131:-1 11/11 None
GENEFINDER00000021245 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5816634:5818131:-1 11/11 None
C32F10.4.1 ENSEMBL cdna:known chromosome:CEL140:I:5816600:5818138:-1 gene:C32F10.4 11/11 None
C32F10.4.2 ENSEMBL cdna:known chromosome:CEL140:I:5816634:5818140:-1 gene:C32F10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5816633-5818131(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32F10.4
Entrez Gene 172248 Entrez gene
SwissProt O01681 EMBL-EBI
WormBase CE08541 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24267 Hypothetical protein C32F10.4 [Caenorhabditis elegans] ref|NP_491687.1| C32F10.4 [Caenorhabditis elegans] 1.0E-149
blast CAE60457 Hypothetical protein CBG04065 [Caenorhabditis briggsae] 5.0E-95
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491687.1 4 39-61,66-88,101-123,157-174
C32F10.4.1 4 39-61,66-88,101-123,157-174
C32F10.4.2 4 39-61,66-88,101-123,157-174
SNAP00000021234 4 39-61,66-88,101-123,157-174
GENEFINDER00000021245 4 39-61,66-88,101-123,157-174
Sequence
Target Sequence 
>C. ELEGANS:185969_S_AT
ggttataatctcatgtttcttcctaactgttagtattattgttgcactcgtccaaatgcg
atatatgatgattctctacaacttcctcagaagaaatgcatacagattacctcaacatca
ggcgtcaaattacaatgttcaatacattgtaccaacaccaatgagtcccagtggtatgcc
accaccaccaagatataccgcctacgccccaccaatgtcaagtcaaactgctcctccgat
tcctacaaaacaacaacctgttgatagcgagagttcgacgacgtcggcatcaacgaggag
tggttctttcgtatcgacaccgattttgacagttgccaatagtgaaccaacaggagctga
caattcggcgtttacaaatgatgaagatccaacccgaccacgtgcaacttctcggccgcc
tgcatatagtgacgtggtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTATAATCTCATGTTTCTTCCTA 108 507 483 Antisense
ATTGTTGCACTCGTCCAAATGCGAT 652 3 520 Antisense
GATTCTCTACAACTTCCTCAGAAGA 524 431 552 Antisense
TGAGTCCCAGTGGTATGCCACCACC 460 567 644 Antisense
CAATGTCAAGTCAAACTGCTCCTCC 420 183 695 Antisense
CTCCTCCGATTCCTACAAAACAACA 608 233 713 Antisense
GACGACGTCGGCATCAACGAGGAGT 397 373 759 Antisense
GGAGTGGTTCTTTCGTATCGACACC 270 517 779 Antisense
TCGACACCGATTTTGACAGTTGCCA 628 599 796 Antisense
AGGAGCTGACAATTCGGCGTTTACA 548 55 834 Antisense
GCCGCCTGCATATAGTGACGTGGTT 577 285 897 Antisense
Sequence Source Affymetrix Proprietary Database