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[185959_at]

Description H09G03.2 /REP_DB=WormBase Gene ID /WP=CE19485 /TR=Q9TZ61 /GB=AAC68809.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 29      6grp_total > 29      4grp_total > 30      6grp_cgc > 02      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 333
1 2 3 4 range
A 365
(3)
P 		104.4
(-2.2)
A 		62.1
(-3)
A 		175.4
(-0.8)
P 		303
B 315.2
(3)
P 		146
(-0.9)
A 		56.5
(-3)
A 		265.1
(1.8)
P 		259
C 389.8
(3)
P 		116.8
(-2.1)
P 		71.4
(-3)
A 		238.1
(0.1)
P 		318
avr 356.7
(3) 		122.4
(-1.8) 		63.3
(-3) 		226.2
(0.3) 		293

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 372
1 2 3 4 5 6 range
A 202.4
(0.3)
A 		276.7
(3)
P 		113.7
(-3)
A 		174.1
(-0.8)
P 		217.5
(0.8)
P 		206.9
(0.4)
P 		163
B 293.9
(3)
P 		244.4
(1.6)
P 		77.4
(-3)
A 		210.8
(0.7)
P 		190.1
(0.1)
P 		210.1
(0.7)
P 		217
C 449.6
(3)
P 		201
(-1.8)
P 		139
(-3)
P 		153.8
(-2.7)
A 		328.3
(0.7)
P 		140.1
(-3)
A 		311
avr 315.3
(3) 		240.7
(0.8) 		110
(-3) 		179.6
(-1) 		245.3
(1) 		185.7
(-0.8) 		205
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 132.9
(3)
P 		22.7
(-2.7)
A 		16.2
(-3)
A 		25.7
(-2.5)
P 		117
B 100.5
(3)
P 		36.1
(-1.4)
A 		13.1
(-3)
A 		56.5
(-0)
P 		87
C 129
(3)
P 		33.6
(-2.5)
P 		24.9
(-3)
A 		40.1
(-2.1)
P 		104
avr 120.8
(3) 		30.8
(-2.3) 		18.1
(-3) 		40.8
(-1.7) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 37.3
(-2)
A 		98.4
(3)
P 		24.8
(-3)
A 		44.2
(-1.4)
P 		65.6
(0.3)
P 		50.4
(-0.9)
P 		74
B 102.2
(3)
P 		81.4
(1.5)
P 		18.8
(-3)
A 		55.8
(-0.3)
P 		77.2
(1.2)
P 		78.3
(1.3)
P 		83
C 166.6
(3)
P 		61.3
(-1.7)
P 		35.4
(-2.8)
P 		30.9
(-3)
A 		106.2
(0.3)
P 		46.9
(-2.3)
A 		136
avr 102
(3) 		80.4
(1.3) 		26.3
(-3) 		43.6
(-1.6) 		83
(1.5) 		58.5
(-0.4) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185959_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6589
Sequence Type Exemplar sequence
Representative Public ID H09G03.2 NCBI
Target Description H09G03.2 /REP_DB=WormBase Gene ID /WP=CE19485 /TR=Q9TZ61 /GB=AAC68809.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171097(10), NM_171096(10), NM_065271(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171097 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-8) (frm-8) mRNA, complete cds. 10/11 None
NM_171096 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-8) (frm-8) mRNA, complete cds. 10/11 None
NM_065271 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-8) (frm-8) mRNA, complete cds. 10/11 None
GENEFINDER00000003925 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3201701:3219132:-1 10/11 None
H09G03.2a ENSEMBL cdna:known chromosome:CEL140:III:3200789:3208873:-1 gene:H09G03.2 10/11 None
H09G03.2b.1 ENSEMBL cdna:known chromosome:CEL140:III:3200940:3219139:-1 gene:H09G03.2 10/11 None
H09G03.2b.2 ENSEMBL cdna:known chromosome:CEL140:III:3200940:3219141:-1 gene:H09G03.2 10/11 None
H09G03.2c ENSEMBL cdna:known chromosome:CEL140:III:3201830:3208885:-1 gene:H09G03.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3202746-3208216(-) 98.6 99.76
Public Domain and Genome References
Ensembl H09G03.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM48544 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 8, isoform a [Caenorhabditis elegans] ref|NP_741110.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-8) [Caenorhabditis elegans] 0.0
blast AAN63403 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 8, isoform c [Caenorhabditis elegans] ref|NP_497672.2| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-8) [Caenorhabditis elegans] 0.0
blast AAM48545 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 8, isoform b [Caenorhabditis elegans] ref|NP_741109.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-8) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000159 EMBL-EBI RA domain 3.6E-4
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 4.4E-9
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 6.9E-12
Pfam IPR000159 EMBL-EBI RA domain 3.6E-4
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 4.4E-9
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 3.2E-6
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 6.9E-12
Sequence
Target Sequence 
>C. ELEGANS:185959_AT
agtaaccctcaccagggtctggaatttttggtggataaggatgatttggacgacttggtt
atgtatatagtgggttatcataaggtaacctacgaaaaagatctgctcctagaaatcgac
gactctcctccaaaacttcgagaactccccgacaaagcccctccgtactcggcaatacat
accgtagtaaaatcggactggaattacagtgaaacttcatctcaaactgaaaaaccattt
gatccaactgatgggcctccaagctatgaaattgccaactcatttgtgcagattgctttt
gaaaatgagcaaaatgaggatcacttcgatacggagatttcccgacaaaatggagaaagc
ttcacaccaaaacgggcacattttgagccgaaaaagttgctccgagccactgactcgttg
ctcatcaagaattcacgggatcttcatgcaaaagagctcgaaattcaaaatggtggctca
cagaatgatgagagctctgatacggaggattcgtcgttttcttcgccgttaagaatttcg
aaaaacttcgtaaattttttttttcgaattttaaaaattccaggatttcatacacttttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTAACCCTCACCAGGGTCTGGAAT 602 1 1459 Antisense
TCCTAGAAATCGACGACTCTCCTCC 424 591 1565 Antisense
CCTCCGTACTCGGCAATACATACCG 61 265 1618 Antisense
TTGATCCAACTGATGGGCCTCCAAG 283 705 1697 Antisense
GGCCTCCAAGCTATGAAATTGCCAA 538 543 1712 Antisense
GCCAACTCATTTGTGCAGATTGCTT 207 279 1732 Antisense
GATCACTTCGATACGGAGATTTCCC 698 419 1777 Antisense
GCCGAAAAAGTTGCTCCGAGCCACT 452 285 1845 Antisense
AGAATTCACGGGATCTTCATGCAAA 149 75 1886 Antisense
TACGGAGGATTCGTCGTTTTCTTCG 181 645 1959 Antisense
AATTCCAGGATTTCATACACTTTTT 133 179 2034 Antisense
Sequence Source Affymetrix Proprietary Database