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[185956_at]

Description Y71H2B.5 /REP_DB=WormBase Gene ID /WP=CE28268 /TR=Q9N4F1 /GB=AAF36034.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 69      6grp_highest_nonorm > 91      6grp_total > 79      4grp_total > 55      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 218.4
(3)
P 		131
(-3)
A 		191.5
(1.2)
A 		176.9
(0.2)
A 		87
B 192.8
(3)
P 		157.4
(-0.3)
A 		128.1
(-3)
A 		140.6
(-1.8)
A 		65
C 216.6
(3)
P 		209.1
(2.1)
A 		189.4
(-0.2)
A 		165
(-3)
P 		52
avr 209.3
(3) 		165.8
(-2.4) 		169.7
(-1.9) 		160.8
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 184.6
(1)
P 		197.6
(1.8)
P 		119.6
(-3)
P 		165
(-0.2)
P 		216.4
(3)
P 		215.9
(3)
P 		97
B 205
(0.7)
P 		163.8
(-2.3)
P 		153.9
(-3)
P 		174.8
(-1.5)
P 		200.4
(0.4)
P 		235.9
(3)
P 		82
C 171.8
(-3)
P 		198.5
(2.7)
P 		199.9
(3)
P 		183.1
(-0.6)
P 		187.7
(0.4)
P 		197.8
(2.6)
P 		28
avr 187.1
(-0) 		186.6
(-0.1) 		157.8
(-3) 		174.3
(-1.3) 		201.5
(1.5) 		216.5
(3) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 79.5
(3)
P 		28.4
(-2.7)
A 		50.1
(-0.3)
A 		25.9
(-3)
A 		54
B 61.5
(3)
P 		38.9
(-1.3)
A 		29.7
(-3)
A 		30
(-2.9)
A 		32
C 71.7
(3)
P 		60.1
(1.4)
A 		66
(2.2)
A 		27.8
(-3)
P 		44
avr 70.9
(3) 		42.5
(-1) 		48.6
(-0.1) 		27.9
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 34
(-1.9)
P 		70.3
(3)
P 		26
(-3)
P 		41.8
(-0.9)
P 		65.3
(2.3)
P 		52.6
(0.6)
P 		44
B 71.3
(1)
P 		54.5
(-1)
P 		37.4
(-3)
P 		46.3
(-1.9)
P 		81.4
(2.2)
P 		88
(3)
P 		51
C 63.7
(2.5)
P 		60.6
(1.9)
P 		50.9
(-0.1)
P 		36.8
(-3)
P 		60.7
(1.9)
P 		66.2
(3)
P 		29
avr 56.3
(0.5) 		61.8
(1.6) 		38.1
(-3) 		41.6
(-2.3) 		69.1
(3) 		68.9
(3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185956_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6625
Sequence Type Exemplar sequence
Representative Public ID Y71H2B.5 NCBI
Target Description Y71H2B.5 /REP_DB=WormBase Gene ID /WP=CE28268 /TR=Q9N4F1 /GB=AAF36034.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065188(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065188 NCBI Caenorhabditis elegans Y71H2B.5 (Y71H2B.5) mRNA, complete cds. 11/11 None
SNAP00000014009 ENSEMBL cdna:SNAP chromosome:CEL140:III:2604229:2618377:1 11/11 None
GENEFINDER00000014040 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2613034:2618708:1 11/11 None
Y71H2B.5 ENSEMBL cdna:known chromosome:CEL140:III:2609284:2618845:1 gene:Y71H2B.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2604228-2618377(+) 98.35 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2B.5
Entrez Gene 175380 Entrez gene
SwissProt Q9N4F1 EMBL-EBI
WormBase CE32548 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK93869 Hypothetical protein Y71H2B.5 [Caenorhabditis elegans] ref|NP_497589.2| Y71H2B.5 [Caenorhabditis elegans] 0.0
blast CAE64989 Hypothetical protein CBG09825 [Caenorhabditis briggsae] 0.0
blast XP_795634 PREDICTED: similar to putative cytoplasmic protein of ancient origin (92.4 kD) (3D707) [Strongylocentrotus purpuratus] 1.0E-106
blast XP_795634 PREDICTED: similar to putative cytoplasmic protein of ancient origin (92.4 kD) (3D707) [Strongylocentrotus purpuratus] 1.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR000541 EMBL-EBI Protein of unknown function UPF0021 9.5E-18
Pfam IPR000541 EMBL-EBI Protein of unknown function UPF0021 9.5E-18
Pfam IPR000541 EMBL-EBI Protein of unknown function UPF0021 9.5E-18
Sequence
Target Sequence 
>C. ELEGANS:185956_AT
tttacggcactatcaacaacgctgtaataaggctagaagcacctccttcgaactcggtgc
aatcaccgttgatccaggatctgcagaatacaatccacgcccattaatcgagtattgtcg
acggctcaatattgattatttctacgaagagcaggatattattggagccgcccgaaaaac
gcccggactccgatcgatttgcgcgttttgtagtcgaatgaagcgtggccgactcgccgc
cgccgcacaattacacggatggaatgttctggcgatgggacaacatttggatgatcttgc
tgaaagcttcttcattgccgcttttcagaatggaaatttgagcacgatgaaggctcagta
tacgacgaaagacggagctcttcgagtcattcgaccgctggtgatggttcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTACGGCACTATCAACAACGCTGT 172 671 3930 Antisense
TAAGGCTAGAAGCACCTCCTTCGAA 178 635 3957 Antisense
GTGCAATCACCGTTGATCCAGGATC 457 481 3986 Antisense
GATCTGCAGAATACAATCCACGCCC 111 419 4007 Antisense
AATCCACGCCCATTAATCGAGTATT 408 167 4021 Antisense
GCAGGATATTATTGGAGCCGCCCGA 596 311 4080 Antisense
CTCCGATCGATTTGCGCGTTTTGTA 389 235 4117 Antisense
TCGAATGAAGCGTGGCCGACTCGCC 312 601 4143 Antisense
GAAAGCTTCTTCATTGCCGCTTTTC 596 359 4231 Antisense
GACGGAGCTCTTCGAGTCATTCGAC 629 375 4300 Antisense
CATTCGACCGCTGGTGATGGTTCGC 445 217 4317 Antisense
Sequence Source Affymetrix Proprietary Database