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[185955_at]

Description C28C12.6 /REP_DB=WormBase Gene ID /WP=CE04113 /TR=Q18278 /GB=AAB37549.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 10      4grp_highest_8_8 > 35      4grp_high_8_8 > 48      6grp_highest_nonorm > 14      6grp_total > 50      4grp_total > 55      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1939
1 2 3 4 range
A 3940.3
(3)
P 		2083.7
(-3)
P 		3082.3
(0.2)
P 		2567.6
(-1.4)
P 		1857
B 3484.2
(3)
P 		2578.3
(-1.1)
P 		2960.8
(0.7)
P 		2142.7
(-3)
P 		1342
C 3536.5
(3)
P 		2486.4
(-1.1)
P 		2788
(0.1)
P 		2001.8
(-3)
P 		1535
avr 3653.7
(3) 		2382.8
(-2.4) 		2943.7
(-0) 		2237.4
(-3) 		1416

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2963
1 2 3 4 5 6 range
A 4815.3
(3)
P 		3160.8
(-0.4)
P 		3671.9
(0.7)
P 		3917.6
(1.2)
P 		2689.3
(-1.3)
P 		1852.8
(-3)
P 		2963
B 4449.5
(3)
P 		3220.6
(0.1)
P 		3963.1
(1.8)
P 		3325.4
(0.3)
P 		2339.7
(-2)
P 		1921.2
(-3)
P 		2528
C 3282.6
(-0.4)
P 		3258.1
(-0.5)
P 		3978.7
(1.2)
P 		4706.5
(3)
P 		2426
(-2.5)
P 		2225.8
(-3)
P 		2481
avr 4182.5
(3) 		3213.2
(0.3) 		3871.2
(2.1) 		3983.2
(2.5) 		2485
(-1.7) 		1999.9
(-3) 		2183
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1097
1 2 3 4 range
A 1434.7
(3)
P 		452.3
(-2.6)
P 		805.8
(-0.6)
P 		375.8
(-3)
P 		1059
B 1111.1
(3)
P 		636.8
(-1.3)
P 		686.1
(-0.9)
P 		456.6
(-3)
P 		655
C 1170.7
(3)
P 		714.9
(-0.3)
P 		971.7
(1.6)
P 		337.5
(-3)
P 		833
avr 1238.8
(3) 		601.3
(-1.5) 		821.2
(0) 		390
(-3) 		849

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1097
1 2 3 4 5 6 range
A 887.7
(0.9)
P 		1124.1
(3)
P 		799.7
(0.1)
P 		993.6
(1.8)
P 		811.5
(0.2)
P 		451.1
(-3)
P 		673
B 1548.2
(3)
P 		1072
(-0.4)
P 		962.4
(-1.2)
P 		880.8
(-1.8)
P 		950.6
(-1.3)
P 		716.4
(-3)
P 		832
C 1216.7
(3)
P 		994.2
(0.2)
P 		1012.6
(0.4)
P 		946.5
(-0.4)
P 		785
(-2.5)
P 		745.1
(-3)
P 		472
avr 1217.5
(3) 		1063.4
(1.4) 		924.9
(-0) 		940.3
(0.1) 		849
(-0.8) 		637.5
(-3) 		580
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185955_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8656
Sequence Type Exemplar sequence
Representative Public ID C28C12.6 NCBI
Target Description C28C12.6 /REP_DB=WormBase Gene ID /WP=CE04113 /TR=Q18278 /GB=AAB37549.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171399(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171399 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-10) (spp-10) mRNA, complete cds. 10/11 A 
SNAP00000012164 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8479770:8480558:1 9/11 None
GENEFINDER00000012176 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8479770:8480536:1 9/11 None
C28C12.7b ENSEMBL cdna:known chromosome:CEL140:IV:8477165:8480621:1 gene:C28C12.7 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177651 2 1 NM_171399 185955_at (10)
NM_171400 173276_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8479468-8480564(+) 100.0 100.0
Public Domain and Genome References
Ensembl C28C12.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37549 Saposin-like protein family protein 10, isoform b [Caenorhabditis elegans] ref|NP_741465.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 0.0
blast AAB37550 Saposin-like protein family protein 10, isoform a [Caenorhabditis elegans] ref|NP_741466.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 1.0E-126
blast AAB37549 Saposin-like protein family protein 10, isoform b [Caenorhabditis elegans] ref|NP_741465.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 3.0E-94
blast CAE71555 Hypothetical protein CBG18502 [Caenorhabditis briggsae] 2.0E-93
blast AAB37549 Saposin-like protein family protein 10, isoform b [Caenorhabditis elegans] ref|NP_741465.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 5.0E-92
blast CAE71554 Hypothetical protein CBG18501 [Caenorhabditis briggsae] 7.0E-72
blast CAE71554 Hypothetical protein CBG18501 [Caenorhabditis briggsae] 3.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR007856 EMBL-EBI Saposin-like type B, 1 1.8E-11
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 1.7E-9
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 4.7E-11
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 7.9E-11
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 7.9E-11
Sequence
Target Sequence 
>C. ELEGANS:185955_AT
agggaatttgatactctcgccatgtcctgtttcgaatgcaagtttgttgttgaagacatg
caaacagacatgatcactaacagaaagaaactttccgatgatgtccgtgatttcgcatgc
tacaagattgtcacagccaacatgactgattcatgtattgatttcttggatttatatctc
ccaactgttattcagatgacagttgaacaggcaactccacaaggagtttgtctagctaac
aagtgttgtccagcaagttcagttgccccacttcgtgatttcacattccaagagattcaa
gctgaaaaatgtccgaaaatgatggaattggaaacatatttgagctcaaaccttgttgga
agtgccatggagaaattcttcgaaaactcactgactgagactttctgctctggattatta
tcattcttccaaccaagttgtcaacaaatcatgtcatctgttgctccaagatttgtcagt
ttgaccgcagtcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGGAATTTGATACTCTCGCCATGT 244 59 67 Antisense
TCGCCATGTCCTGTTTCGAATGCAA 336 599 83 Antisense
GAAACTTTCCGATGATGTCCGTGAT 65 357 153 Antisense
GTCCGTGATTTCGCATGCTACAAGA 525 475 169 Antisense
GATTTATATCTCCCAACTGTTATTC 52 429 235 Antisense
GTTGTCCAGCAAGTTCAGTTGCCCC 519 437 311 Antisense
TGCCCCACTTCGTGATTTCACATTC 543 585 330 Antisense
TGACTGAGACTTTCTGCTCTGGATT 490 569 458 Antisense
GATTATTATCATTCTTCCAACCAAG 499 429 479 Antisense
AAATCATGTCATCTGTTGCTCCAAG 316 117 512 Antisense
GATTTGTCAGTTTGACCGCAGTCCT 387 427 536 Antisense
Sequence Source Affymetrix Proprietary Database