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[185953_at]

Description H32C10.3 /REP_DB=WormBase Gene ID /WP=CE21005 /TR=Q9TYQ6 /GB=AAD12801.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 25      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 16.4
(-3)
A 		56.5
(3)
A 		53.4
(2.5)
A 		55.2
(2.8)
A 		40
B 32.8
(-3)
A 		76.6
(3)
A 		37.6
(-2.3)
A 		62.5
(1.1)
A 		44
C 30.7
(-1)
A 		17.8
(-3)
A 		33.8
(-0.5)
A 		56.3
(3)
A 		39
avr 26.6
(-3) 		50.3
(1.5) 		41.6
(-0.1) 		58
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 67.1
(3)
A 		8
(-3)
A 		8.5
(-2.9)
A 		24.7
(-1.3)
A 		59.4
(2.2)
A 		50.1
(1.3)
A 		59
B 8.8
(-3)
A 		34.5
(-0.5)
A 		45.7
(0.6)
A 		21.3
(-1.8)
A 		69.9
(3)
P 		63.8
(2.4)
P 		61
C 11.5
(-2.7)
A 		45.4
(0.9)
A 		8.2
(-3)
A 		56.1
(2)
A 		31.7
(-0.5)
P 		65.3
(3)
P 		57
avr 29.1
(-1.7) 		29.3
(-1.7) 		20.8
(-3) 		34
(-1) 		53.7
(2.1) 		59.7
(3) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 6
(-3)
A 		12.3
(1.7)
A 		14
(3)
A 		8.1
(-1.4)
A 		8
B 10.5
(-1.9)
A 		18.9
(3)
A 		8.7
(-3)
A 		13.3
(-0.3)
A 		10
C 10.2
(1.6)
A 		5.1
(-3)
A 		11.8
(3)
A 		9.5
(0.9)
A 		7
avr 8.9
(-3) 		12.1
(3) 		11.5
(1.9) 		10.3
(-0.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 12.4
(1)
A 		2.9
(-2.6)
A 		1.8
(-3)
A 		6.3
(-1.3)
A 		17.9
(3)
A 		12.2
(0.9)
A 		16
B 3
(-3)
A 		11.5
(-1)
A 		11.1
(-1.1)
A 		5.7
(-2.4)
A 		28.4
(3)
P 		23.8
(1.9)
P 		25
C 4.3
(-2.3)
A 		13.9
(0.6)
A 		2.1
(-3)
A 		11.3
(-0.2)
A 		10.3
(-0.5)
P 		21.9
(3)
P 		20
avr 6.6
(-2.3) 		9.4
(-1.2) 		5
(-3) 		7.8
(-1.8) 		18.9
(2.8) 		19.3
(3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185953_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10926
Sequence Type Exemplar sequence
Representative Public ID H32C10.3 NCBI
Target Description H32C10.3 /REP_DB=WormBase Gene ID /WP=CE21005 /TR=Q9TYQ6 /GB=AAD12801.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068488(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068488 NCBI Caenorhabditis elegans H32C10.3 (H32C10.3) mRNA, complete cds. 11/11 None
SNAP00000008635 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5917626:5921380:1 10/11 None
GENEFINDER00000008647 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5916256:5921380:1 10/11 None
H32C10.3 ENSEMBL cdna:known chromosome:CEL140:IV:5916004:5921380:1 gene:H32C10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5916009-5921386(+) 88.95 100.0
Public Domain and Genome References
Gene Title
Ensembl H32C10.3
Entrez Gene 186797 Entrez gene
SwissProt Q9TYQ6 EMBL-EBI
WormBase CE21005 Wormbase
Functional Annotations
Ortholog
ID Title Organism
YEAST_2:1772903_AT Ankyrin repeat-containing protein similar to Akr1p; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
46872 metal ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD12801 Hypothetical protein H32C10.3 [Caenorhabditis elegans] ref|NP_500889.1| H32C10.3 [Caenorhabditis elegans] 0.0
blast CAE72626 Hypothetical protein CBG19823 [Caenorhabditis briggsae] 0.0
blast CAE64687 Hypothetical protein CBG09465 [Caenorhabditis briggsae] 3.0E-90
blast CAE64687 Hypothetical protein CBG09465 [Caenorhabditis briggsae] 7.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR002110 EMBL-EBI Ankyrin 7.1E-7
Pfam IPR002110 EMBL-EBI Ankyrin 3.5E-6
Pfam IPR002110 EMBL-EBI Ankyrin 2.5E-5
Pfam IPR002110 EMBL-EBI Ankyrin 18.0
Pfam IPR002110 EMBL-EBI Ankyrin 7.8E-5
Pfam IPR001594 EMBL-EBI Zn-finger, DHHC type 6.0E-18
Pfam IPR002110 EMBL-EBI Ankyrin 7.1E-7
Pfam IPR002110 EMBL-EBI Ankyrin 3.5E-6
Pfam IPR002110 EMBL-EBI Ankyrin 2.5E-5
Pfam IPR002110 EMBL-EBI Ankyrin 18.0
Pfam IPR002110 EMBL-EBI Ankyrin 7.8E-5
Pfam IPR001594 EMBL-EBI Zn-finger, DHHC type 6.0E-18
Pfam IPR002110 EMBL-EBI Ankyrin 7.1E-7
Pfam IPR002110 EMBL-EBI Ankyrin 3.5E-6
Pfam IPR002110 EMBL-EBI Ankyrin 2.5E-5
Pfam IPR002110 EMBL-EBI Ankyrin 18.0
Pfam IPR002110 EMBL-EBI Ankyrin 7.8E-5
Pfam IPR001594 EMBL-EBI Zn-finger, DHHC type 6.0E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500889.1 8 9-23,249-266,385-404,419-441,448-470,475-497,586-608,680-702
H32C10.3 8 9-23,249-266,385-404,419-441,448-470,475-497,586-608,680-702
SNAP00000008635 5 240-262,267-284,299-321,388-410,425-447
GENEFINDER00000008647 7 248-265,385-407,411-429,436-458,463-485,575-597,612-634
Sequence
Target Sequence 
>C. ELEGANS:185953_AT
ggaagaatcttcgagctttcgtgtttttctgtcttacaatcttcatgtttcacgtgttct
acgttctattgttgttgtacatgattggtgcctccatgaatgcatccggattcggtaagt
cagttttagaacaagctactgccaaaaatgaagaaggaggcaagttgttgaattcggctg
attattatctgtcaatttttgaggcattaaataattatttccaaccagacttttttttga
cgcgcattttcaaaaatcttgatttagaaaaccggcaaaaacaaaccctaaacgaccatg
gtttgatggtaatcagtctcatcctcgcaatccctcatgttttcggagccggagccatca
cttgtactcaattctcacaaatttctcgtcatgtctctacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAGAATCTTCGAGCTTTCGTGTT 447 531 1751 Antisense
CGTGTTTTTCTGTCTTACAATCTTC 407 245 1770 Antisense
AATCTTCATGTTTCACGTGTTCTAC 81 171 1788 Antisense
CGTGTTCTACGTTCTATTGTTGTTG 252 245 1803 Antisense
GTTGTTGTACATGATTGGTGCCTCC 579 437 1821 Antisense
TGCCTCCATGAATGCATCCGGATTC 622 583 1839 Antisense
ACCAGACTTTTTTTTGACGCGCATT 207 87 1974 Antisense
GATGGTAATCAGTCTCATCCTCGCA 680 407 2055 Antisense
CTCGCAATCCCTCATGTTTTCGGAG 444 231 2074 Antisense
GAGCCATCACTTGTACTCAATTCTC 191 385 2102 Antisense
ACAAATTTCTCGTCATGTCTCTACC 556 111 2127 Antisense
Sequence Source Affymetrix Proprietary Database