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[185947_at]

Description H04J21.1 /REP_DB=WormBase Gene ID /WP=CE20919 /TR=O61207 /GB=AAD34647.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 30      4grp_total > 27      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 81.2
(-3)
A 		151.1
(3)
A 		133.5
(1.5)
A 		97.1
(-1.6)
A 		70
B 97
(3)
A 		67.7
(-0.8)
A 		50.2
(-3)
A 		66.9
(-0.9)
A 		47
C 89.6
(-2)
A 		121.8
(-0.6)
A 		65.8
(-3)
A 		206.2
(3)
P 		140
avr 89.3
(-2.1) 		113.5
(1.5) 		83.2
(-3) 		123.4
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 61.4
(-1)
A 		49.5
(-1.7)
A 		127
(3)
P 		66.9
(-0.6)
A 		79.7
(0.1)
A 		27.8
(-3)
A 		99
B 81.3
(-0.7)
A 		96.9
(1.1)
A 		114.3
(3)
P 		60.2
(-3)
A 		69.7
(-1.9)
A 		60.2
(-3)
A 		54
C 41.5
(-1.4)
A 		143.2
(3)
A 		53.7
(-0.8)
A 		94.6
(0.9)
A 		3.6
(-3)
A 		34.5
(-1.7)
A 		140
avr 61.4
(-0.9) 		96.5
(2.8) 		98.3
(3) 		73.9
(0.5) 		51
(-1.9) 		40.8
(-3) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 29.5
(1.4)
A 		32.8
(2.4)
A 		34.9
(3)
A 		14.2
(-3)
A 		21
B 30.9
(3)
A 		16.7
(-1.4)
A 		11.6
(-3)
A 		14.3
(-2.2)
A 		19
C 29.7
(0.4)
A 		35
(3)
A 		22.9
(-3)
A 		34.8
(2.9)
P 		12
avr 30
(3) 		28.2
(1.8) 		23.1
(-1.7) 		21.1
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 11.3
(-1.7)
A 		17.6
(0.1)
A 		27.7
(3)
P 		17
(-0.1)
A 		24.1
(2)
A 		6.8
(-3)
A 		21
B 28.3
(1.6)
A 		32.2
(3)
A 		27.8
(1.4)
P 		16
(-3)
A 		28.3
(1.6)
A 		22.5
(-0.6)
A 		16
C 15.4
(-1)
A 		43.7
(3)
A 		13.7
(-1.2)
A 		19
(-0.5)
A 		1.2
(-3)
A 		11.5
(-1.5)
A 		43
avr 18.3
(-1.4) 		31.2
(3) 		23.1
(0.2) 		17.3
(-1.7) 		17.9
(-1.5) 		13.6
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185947_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7795
Sequence Type Exemplar sequence
Representative Public ID H04J21.1 NCBI
Target Description H04J21.1 /REP_DB=WormBase Gene ID /WP=CE20919 /TR=O61207 /GB=AAD34647.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065142(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065142 NCBI Caenorhabditis elegans H04J21.1 (H04J21.1) mRNA, complete cds. 11/11 None
H04J21.1 ENSEMBL cdna:known chromosome:CEL140:III:2366872:2372643:-1 gene:H04J21.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032514 5/11 Cross Hyb Matching Probes None
SNAP00000039212 5/11 Cross Hyb Matching Probes None
GENEFINDER00000039220 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2366871-2372643(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H04J21.1
Entrez Gene 186681 Entrez gene
SwissProt O61207 EMBL-EBI
WormBase CE20919 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6979 response to oxidative stress inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4601 peroxidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD34647 Hypothetical protein H04J21.1 [Caenorhabditis elegans] ref|NP_497543.1| H04J21.1 [Caenorhabditis elegans] 0.0
blast AAB54270 Hypothetical protein R155.4 [Caenorhabditis elegans] ref|NP_497486.2| R155.4 [Caenorhabditis elegans] 1.0E-126
blast AAK29876 Hypothetical protein Y22D7AR.12 [Caenorhabditis elegans] ref|NP_497451.1| Y22D7AR.12 [Caenorhabditis elegans] 6.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 5.9E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497543.1 1 854-876
H04J21.1 1 854-876
Sequence
Target Sequence 
>C. ELEGANS:185947_AT
ggatgccgggccaagattctacaatcttgcaaagaaagaagcggcgagtggattttggga
taagtaccttcttctcataatcacaatcgtgatcttaatcatcgtggttgtttctatcgg
agttgcaatcccatttgcgattaaatacaagaagaagaaagctgccgagaagttggagga
tttggagaagccggtggacaagaagaagaaaaagggaaagaagtcgaaagattccaaaaa
gtctgctgagaagaagagtaccgagaagagctctgccatgacagctccaacgaattctgc
tcaacaactagaagtcctccactcgcacgtcgaggagaacgagaagctgggaaggttgga
caaacggatttccgagaaaatcggagttgacgccaaatttattcgagtgacctacaatgg
acgtcccattgaccctatggacacaccgtattcacttggattatcacataccgccaattt
tcatatgttcttcaaaccgtcgcctggaccctattgtagtcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGCCGGGCCAAGATTCTACAAT 558 513 2499 Antisense
GTACCTTCTTCTCATAATCACAATC 82 459 2562 Antisense
CGTGGTTGTTTCTATCGGAGTTGCA 228 245 2601 Antisense
GTACCGAGAAGAGCTCTGCCATGAC 583 457 2756 Antisense
AGCTCCAACGAATTCTGCTCAACAA 449 81 2781 Antisense
TCAACAACTAGAAGTCCTCCACTCG 133 631 2799 Antisense
CCTCCACTCGCACGTCGAGGAGAAC 321 267 2814 Antisense
GTCCCATTGACCCTATGGACACACC 462 475 2921 Antisense
GGACACACCGTATTCACTTGGATTA 225 527 2937 Antisense
GGATTATCACATACCGCCAATTTTC 580 507 2956 Antisense
TCGCCTGGACCCTATTGTAGTCAAT 215 599 2998 Antisense
Sequence Source Affymetrix Proprietary Database