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[185936_at]

Description R12C12.2 /REP_DB=WormBase Gene ID /WP=CE02847 /TR=Q21961 /GB=AAC46781.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 97      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 85      6grp_total > 96      4grp_total > 55      6grp_highest_sub > In      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 731.2
(3)
P 		654.9
(-0.7)
P 		606.6
(-3)
P 		662.6
(-0.3)
P 		125
B 711.4
(3)
P 		547
(-3)
P 		643.6
(0.5)
P 		609.6
(-0.7)
P 		164
C 766.9
(3)
P 		568.1
(-2.3)
P 		637.7
(-0.5)
P 		543.5
(-3)
P 		223
avr 736.5
(3) 		590
(-3) 		629.3
(-1.4) 		605.2
(-2.4) 		147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 466
1 2 3 4 5 6 range
A 611.6
(-2)
P 		679.2
(-0.9)
P 		547.5
(-3)
P 		752
(0.3)
P 		755.8
(0.4)
P 		918.3
(3)
P 		371
B 649.1
(-0.5)
P 		552.9
(-2.2)
P 		504.7
(-3)
P 		626.6
(-0.9)
P 		714.1
(0.6)
P 		858
(3)
P 		353
C 540.4
(-3)
P 		553.1
(-2.8)
P 		716.6
(-0.5)
P 		862.7
(1.5)
P 		839.5
(1.2)
P 		970.6
(3)
P 		430
avr 600.4
(-2.8) 		595.1
(-2.9) 		589.6
(-3) 		747.1
(-0.1) 		769.8
(0.3) 		915.6
(3) 		326
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 266.2
(3)
P 		142.2
(-1.4)
P 		158.6
(-0.8)
P 		97
(-3)
P 		169
B 226.9
(3)
P 		135.1
(-2.7)
P 		149.1
(-1.8)
P 		129.9
(-3)
P 		97
C 253.8
(3)
P 		163.3
(-0.3)
P 		222.3
(1.8)
P 		91.6
(-3)
P 		162
avr 249
(3) 		146.9
(-1.3) 		176.7
(-0) 		106.2
(-3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 212
1 2 3 4 5 6 range
A 112.7
(-3)
P 		241.6
(3)
P 		119.2
(-2.7)
P 		190.7
(0.6)
P 		228.1
(2.4)
P 		223.6
(2.2)
P 		129
B 225.9
(0.1)
P 		184
(-1.1)
P 		122.6
(-3)
P 		166
(-1.7)
P 		290.1
(2.1)
P 		319.9
(3)
P 		197
C 200.3
(-1.8)
P 		168.8
(-3)
P 		182.4
(-2.5)
P 		173.5
(-2.8)
P 		271.7
(1)
P 		324.9
(3)
P 		156
avr 179.6
(-1.5) 		198.1
(-0.7) 		141.4
(-3) 		176.7
(-1.6) 		263.3
(1.9) 		289.5
(3) 		148
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185936_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5468
Sequence Type Exemplar sequence
Representative Public ID R12C12.2 NCBI
Target Description R12C12.2 /REP_DB=WormBase Gene ID /WP=CE02847 /TR=Q21961 /GB=AAC46781.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062807(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062807 NCBI Caenorhabditis elegans associated with RAN (nuclear import/export) function family member (ran-5) (ran-5) mRNA, complete cds. 11/11 None
SNAP00000025497 ENSEMBL cdna:SNAP chromosome:CEL140:II:6048066:6049376:1 11/11 None
GENEFINDER00000025595 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6048066:6049376:1 11/11 None
R12C12.2.1 ENSEMBL cdna:known chromosome:CEL140:II:6048061:6049631:1 gene:R12C12.2 11/11 None
R12C12.2.2 ENSEMBL cdna:known chromosome:CEL140:II:6048064:6049631:1 gene:R12C12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6048062-6049373(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ran-5
Ensembl R12C12.2
Entrez Gene 174011 Entrez gene
SwissProt Q21961 EMBL-EBI
WormBase CE02847 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495208 associated with RAN (nuclear import/export) function family member (ran-5) [Caenorhabditis elegans] gb|AAC46781.1| Associated with ran (nuclear import/export) function protein 5 [Caenorhabditis elegans] 1.0E-156
blast CAE59243 Hypothetical protein CBG02569 [Caenorhabditis briggsae] 1.0E-125
Sequence
Target Sequence 
>C. ELEGANS:185936_AT
agcagcatgccggaacgagttcagtcgaaatcaccaccggggaagagaacgatactaata
ttttccaagctccttgcaaaatttgggcgtttgataaattgaaaaatgcctactctgaaa
agggtgtctgcaatctcaggatcaacaaacgagtcgagaacggactgacgcaccaccgaa
ttgtggcaagaacatcttccggtactcttcgtgtcatcataaattccaaaattttctctg
atatgcttttggagcgggtcgacaagagaattcgaatttccgcaatgggaccagagatca
gcggtgttcagattttccttctaaaaatcggattcaccaaaactgagacgatccccgatt
cggatatattctacaatattatgagcgacctgctgaaattggagaagggagagaatagtc
ggaagaggaaagcggattgtgatctgaacgcttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAGCATGCCGGAACGAGTTCAGT 625 77 416 Antisense
CGAGTTCAGTCGAAATCACCACCGG 410 249 431 Antisense
GATACTAATATTTTCCAAGCTCCTT 13 425 466 Antisense
AAGGGTGTCTGCAATCTCAGGATCA 589 159 535 Antisense
GACTGACGCACCACCGAATTGTGGC 367 373 578 Antisense
GCAAGAACATCTTCCGGTACTCTTC 345 319 601 Antisense
GGTACTCTTCGTGTCATCATAAATT 649 503 616 Antisense
TATGCTTTTGGAGCGGGTCGACAAG 497 649 657 Antisense
TCAGCGGTGTTCAGATTTTCCTTCT 509 623 713 Antisense
CCAAAACTGAGACGATCCCCGATTC 211 275 752 Antisense
GCGGATTGTGATCTGAACGCTTCAA 226 293 847 Antisense
Sequence Source Affymetrix Proprietary Database