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[185928_s_at]

Description Y71F9B.7 /REP_DB=WormBase Gene ID /WP=CE22877 /TR=Q9N2L7 /GB=AAF36014.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 32      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 7.5
(-3)
A 		139.5
(0.8)
A 		144.2
(1)
P 		214
(3)
P 		207
B 22.8
(-3)
A 		195.7
(3)
A 		194.6
(3)
P 		170.2
(2.1)
P 		173
C 15.7
(-3)
A 		80.3
(-0.7)
A 		171.3
(2.6)
P 		181.8
(3)
P 		166
avr 15.3
(-3) 		138.5
(1.3) 		170
(2.4) 		188.7
(3) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 563
1 2 3 4 5 6 range
A 109.4
(-2.9)
A 		100.4
(-3)
A 		97.2
(-3)
P 		182
(-2)
P 		384.2
(0.3)
P 		612.4
(3)
P 		515
B 63.3
(-3)
A 		86.9
(-2.7)
A 		144.5
(-2.1)
P 		206.1
(-1.4)
P 		365.5
(0.3)
P 		615.5
(3)
P 		552
C 52.1
(-3)
A 		60.6
(-2.9)
A 		84.3
(-2.6)
A 		243.7
(-0.7)
P 		392.2
(1.1)
P 		550.8
(3)
P 		499
avr 74.9
(-3) 		82.6
(-2.9) 		108.7
(-2.6) 		210.6
(-1.4) 		380.6
(0.5) 		592.9
(3) 		518
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 2.7
(-3)
A 		30.3
(1.7)
A 		37.7
(3)
P 		31.3
(1.9)
P 		35
B 7.3
(-3)
A 		48.3
(3)
A 		45.1
(2.5)
P 		36.3
(1.2)
P 		41
C 5.2
(-3)
A 		23.1
(-1)
A 		59.7
(3)
P 		30.7
(-0.2)
P 		55
avr 5.1
(-3) 		33.9
(1.1) 		47.5
(3) 		32.8
(0.9) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 211
1 2 3 4 5 6 range
A 20.2
(-3)
A 		35.7
(-2.3)
A 		21.2
(-3)
P 		46.2
(-1.8)
P 		115.9
(1.5)
P 		149.1
(3)
P 		129
B 22
(-3)
A 		28.9
(-2.8)
A 		35.1
(-2.6)
P 		54.6
(-2.1)
P 		148.5
(0.7)
P 		229.5
(3)
P 		208
C 19.3
(-3)
A 		18.5
(-3)
A 		21.5
(-2.9)
A 		49
(-1.9)
P 		126.9
(0.9)
P 		184.4
(3)
P 		166
avr 20.5
(-3) 		27.7
(-2.7) 		25.9
(-2.8) 		49.9
(-1.9) 		130.4
(0.9) 		187.7
(3) 		167
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185928_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.250
Sequence Type Exemplar sequence
Representative Public ID Y71F9B.7 NCBI
Target Description Y71F9B.7 /REP_DB=WormBase Gene ID /WP=CE22877 /TR=Q9N2L7 /GB=AAF36014.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058635(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058635 NCBI Caenorhabditis elegans POLO Kinase family member (plk-2) (plk-2) mRNA, complete cds. 11/11 A 
SNAP00000013309 ENSEMBL cdna:SNAP chromosome:CEL140:I:2755819:2758205:-1 11/11 None
GENEFINDER00000013356 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2755819:2758205:-1 11/11 None
Y71F9B.7 ENSEMBL cdna:known chromosome:CEL140:I:2755625:2758205:-1 gene:Y71F9B.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2755818-2758205(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol plk-2
Ensembl Y71F9B.7
Entrez Gene 171838 Entrez gene
SwissProt Q9N2L7 EMBL-EBI
WormBase CE22877 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36014 Polo kinase protein 2 [Caenorhabditis elegans] ref|NP_491036.1| POLO Kinase family member (plk-2) [Caenorhabditis elegans] gb|AAF28314.1| polo-like kinase 2 [Caenorhabditis elegans] sp|Q9N2L7|PLK2_CAEEL Serine/threonine-protein kinase plk-2 (Polo-like kinase-2) 0.0
blast CAE60501 Hypothetical protein CBG04120 [Caenorhabditis briggsae] 0.0
blast CAE64506 Hypothetical protein CBG09233 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-59
hanks 1.9.5 AGC group; AGC Other; DdK2 5.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR000959 EMBL-EBI POLO box duplicated region 1.2E-26
Pfam IPR000959 EMBL-EBI POLO box duplicated region 1.9E-27
Pfam IPR000719 EMBL-EBI Protein kinase 6.8E-84
Sequence
Target Sequence 
>C. ELEGANS:185928_S_AT
gaatcatgctggacactgctggcacccagctgacatatattgagaaaaccgagaaggagc
actatttcgacatggaaagcgcaataccatcgggcctacagaagaagatgacacttctca
agtatttccgatcgtacatgaatgatcatttgttgcaagcaggacagcaagttactcgga
aagtaggagacgatttggctcgccttccgacgcttcgtgtctggttccgaacaaagtcgg
caattgtgcttcatctctccaacggcactgttcagataaactttttcaatgatcacatta
aaatggtgttgtgtccactcatgcaagctgtcaccttcatcgatgagaacaagcgcatgt
tcacgtacaaattttcacacttggctgaaaatggatgtccggagaagttcctccatcgaa
ttcaatacgccaagtgtatgatccaacgactcgtcgaggaacacacgaaagaggagacga
aacacaatgcaccggcggccaatgcagtacgccttccatcgacttccagcaacgtccgtt
tggaatctgcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCATGCTGGACACTGCTGGCAC 654 325 1319 Antisense
CTGGCACCCAGCTGACATATATTGA 200 241 1337 Antisense
GCAATACCATCGGGCCTACAGAAGA 294 323 1399 Antisense
GTAGGAGACGATTTGGCTCGCCTTC 643 453 1501 Antisense
AGTCGGCAATTGTGCTTCATCTCTC 566 61 1553 Antisense
CTCTCCAACGGCACTGTTCAGATAA 17 233 1573 Antisense
TGTGTCCACTCATGCAAGCTGTCAC 387 555 1628 Antisense
GAGAAGTTCCTCCATCGAATTCAAT 556 391 1720 Antisense
AGTGTATGATCCAACGACTCGTCGA 355 59 1751 Antisense
AGACGAAACACAATGCACCGGCGGC 177 71 1793 Antisense
GCAACGTCCGTTTGGAATCTGCAGC 103 321 1847 Antisense
Sequence Source Affymetrix Proprietary Database