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[185924_at]

Description T20D4.19 /REP_DB=WormBase Gene ID /WP=CE13814 /TR=P91476 /GB=AAB37598.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 11      4grp_total > 25      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 7.9
(-3)
A 		53.7
(3)
A 		25.2
(-0.7)
A 		7.7
(-3)
A 		46
B 15.6
(-0.2)
A 		5.6
(-3)
A 		26.9
(3)
A 		13.5
(-0.8)
A 		21
C 7.8
(-2.6)
A 		8
(-2.6)
A 		3.1
(-3)
A 		71.4
(3)
P 		68
avr 10.4
(-3) 		22.4
(0.5) 		18.4
(-0.7) 		30.9
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 32
(3)
A 		11.1
(-2)
A 		31.5
(2.9)
A 		10
(-2.2)
A 		6.7
(-3)
A 		19.3
(-0)
A 		25
B 4
(-2.7)
A 		5.8
(-2.3)
A 		6.3
(-2.2)
A 		5.5
(-2.4)
A 		26.5
(3)
A 		3
(-3)
A 		24
C 3.7
(-3)
A 		48.1
(3)
A 		9.2
(-2.3)
A 		6.9
(-2.6)
A 		5.2
(-2.8)
A 		5.5
(-2.8)
A 		44
avr 13.2
(-0.6) 		21.7
(3) 		15.7
(0.5) 		7.5
(-3) 		12.8
(-0.8) 		9.3
(-2.2) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 2.9
(-2)
A 		11.6
(3)
A 		6.6
(0.1)
A 		1.1
(-3)
A 		11
B 5
(1.5)
A 		1.4
(-3)
A 		6.2
(3)
A 		2.9
(-1.1)
A 		5
C 2.6
(-2.2)
A 		2.3
(-2.3)
A 		1.1
(-3)
A 		12
(3)
P 		11
avr 3.5
(-3) 		5.1
(2.3) 		4.6
(0.7) 		5.3
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 5.9
(1.9)
A 		3.9
(-0.6)
A 		6.8
(3)
A 		2.5
(-2.4)
A 		2
(-3)
A 		4.7
(0.4)
A 		5
B 1.4
(-2.8)
A 		1.9
(-2.5)
A 		1.5
(-2.8)
A 		1.5
(-2.8)
A 		10.8
(3)
A 		1.1
(-3)
A 		10
C 1.4
(-3)
A 		14.7
(3)
A 		2.3
(-2.6)
A 		1.4
(-3)
A 		1.7
(-2.9)
A 		1.9
(-2.8)
A 		13
avr 2.9
(-1.7) 		6.8
(3) 		3.5
(-1) 		1.8
(-3) 		4.8
(0.6) 		2.6
(-2) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185924_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15229
Sequence Type Exemplar sequence
Representative Public ID T20D4.19 NCBI
Target Description T20D4.19 /REP_DB=WormBase Gene ID /WP=CE13814 /TR=P91476 /GB=AAB37598.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071561(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071561 NCBI Caenorhabditis elegans T20D4.19 (T20D4.19) mRNA, complete cds. 11/11 None
SNAP00000008855 ENSEMBL cdna:SNAP chromosome:CEL140:V:3430495:3431272:-1 11/11 None
T20D4.19 ENSEMBL cdna:known chromosome:CEL140:V:3430495:3431272:-1 gene:T20D4.19 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3430494-3431272(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20D4.19
Entrez Gene 188654 Entrez gene
SwissProt P91476 EMBL-EBI
WormBase CE13814 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37598 Hypothetical protein T20D4.19 [Caenorhabditis elegans] ref|NP_503962.1| T20D4.19 [Caenorhabditis elegans] 6.0E-78
blast NP_504867 F10G2.1 [Caenorhabditis elegans] gb|AAG24056.2| Hypothetical protein F10G2.1 [Caenorhabditis elegans] 2.0E-38
blast T29866 hypothetical protein F10G2.1 - Caenorhabditis elegans 2.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 1.7E-38
Sequence
Target Sequence 
>C. ELEGANS:185924_AT
gaaattgttccttctattcatcaccatcagtacagtgcttccgagtttggcagaaggagc
atgtgtgtgtccggctactagaaaagtgaaagactccaattgcacaacggctgatggaat
tcatgcgattaattgttttttgcgactggcggactacgtcgaaaaaatgtatgagcttga
catgactgataagaccgaagttaatgagtttgaaaagtcttgcacctctctcacctcttg
cctggagaccatacaatgtggcctggaaacccagtccgacaagaaaaatgtcaaaatgat
taaaaactattgtgaagcagttgtctatgattccaaagattttgctgagtgctccgaaaa
acttgagaacgagaattccatctgctac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATTGTTCCTTCTATTCATCACC 607 363 15 Antisense
ACCATCAGTACAGTGCTTCCGAGTT 39 87 37 Antisense
GAAGGAGCATGTGTGTGTCCGGCTA 53 335 67 Antisense
AAGACTCCAATTGCACAACGGCTGA 557 153 104 Antisense
TGTTTTTTGCGACTGGCGGACTACG 418 447 148 Antisense
GTTTGAAAAGTCTTGCACCTCTCTC 27 453 222 Antisense
ACCTCTTGCCTGGAGACCATACAAT 623 87 247 Antisense
AATGTGGCCTGGAAACCCAGTCCGA 471 161 269 Antisense
GTGAAGCAGTTGTCTATGATTCCAA 518 481 326 Antisense
CAAAGATTTTGCTGAGTGCTCCGAA 356 193 348 Antisense
TGAGAACGAGAATTCCATCTGCTAC 395 569 378 Antisense
Sequence Source Affymetrix Proprietary Database