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[185917_at]

Description T28C12.2 /REP_DB=WormBase Gene ID /WP=CE14339 /TR=O16697 /GB=AAB66157.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 30      4grp_highest_8_8 > 37      4grp_high_8_8 > 03      6grp_highest_nonorm > 90      6grp_total > 43      4grp_total > 06      6grp_high1_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 290
1 2 3 4 range
A 428.1
(2.6)
P 		232.8
(-3)
P 		282.8
(-1.6)
P 		443.8
(3)
P 		211
B 375.8
(-0)
P 		283.2
(-3)
P 		324.6
(-1.7)
P 		468.9
(3)
P 		186
C 415.3
(0.1)
P 		300.3
(-3)
P 		361.9
(-1.3)
P 		523
(3)
P 		223
avr 406.4
(0.9) 		272.1
(-3) 		323.1
(-1.5) 		478.6
(3) 		207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 140.8
(1)
P 		80.2
(-2)
P 		181
(3)
P 		91.7
(-1.4)
P 		60.1
(-3)
P 		79.6
(-2)
P 		121
B 157.5
(3)
P 		71.4
(-2.3)
P 		134
(1.5)
P 		95.4
(-0.9)
P 		60.9
(-3)
P 		74.1
(-2.2)
P 		97
C 106.4
(0.9)
P 		91.9
(-0)
P 		138.7
(3)
P 		111.9
(1.3)
P 		45.7
(-3)
P 		84.3
(-0.5)
P 		93
avr 134.9
(2) 		81.2
(-1.4) 		151.2
(3) 		99.7
(-0.2) 		55.6
(-3) 		79.3
(-1.5) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 155.9
(3)
P 		50.5
(-3)
P 		73.9
(-1.7)
P 		65
(-2.2)
P 		105
B 119.8
(3)
P 		70
(-3)
P 		75.2
(-2.4)
P 		99.9
(0.6)
P 		50
C 137.5
(3)
P 		86.3
(-3)
P 		126.1
(1.7)
P 		88.2
(-2.8)
P 		51
avr 137.7
(3) 		68.9
(-3) 		91.7
(-1) 		84.4
(-1.6) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 25.9
(-0.8)
P 		28.5
(-0.1)
P 		39.4
(3)
P 		23.3
(-1.5)
P 		18.1
(-3)
P 		19.4
(-2.6)
P 		21
B 54.8
(3)
P 		23.8
(-3)
P 		32.5
(-1.3)
P 		25.3
(-2.7)
P 		24.8
(-2.8)
P 		27.6
(-2.3)
P 		31
C 39.4
(3)
P 		28
(0.2)
P 		35.3
(2)
P 		22.5
(-1.1)
P 		14.8
(-3)
P 		28.2
(0.3)
P 		25
avr 40
(3) 		26.8
(-0.8) 		35.7
(1.8) 		23.7
(-1.7) 		19.2
(-3) 		25.1
(-1.3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185917_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12491
Sequence Type Exemplar sequence
Representative Public ID T28C12.2 NCBI
Target Description T28C12.2 /REP_DB=WormBase Gene ID /WP=CE14339 /TR=O16697 /GB=AAB66157.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072215(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072215 NCBI Caenorhabditis elegans T28C12.2 (T28C12.2) mRNA, complete cds. 11/11 None
SNAP00000017147 ENSEMBL cdna:SNAP chromosome:CEL140:V:6331802:6333579:1 11/11 None
GENEFINDER00000017158 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6331802:6333579:1 11/11 None
T28C12.2 ENSEMBL cdna:known chromosome:CEL140:V:6331791:6333579:1 gene:T28C12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6331801-6333579(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28C12.2
Entrez Gene 179018 Entrez gene
SwissProt O16697 EMBL-EBI
WormBase CE14339 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66157 Hypothetical protein T28C12.2 [Caenorhabditis elegans] ref|NP_504616.1| T28C12.2 [Caenorhabditis elegans] 0.0
blast CAE71864 Hypothetical protein CBG18912 [Caenorhabditis briggsae] 0.0
blast CAE71865 Hypothetical protein CBG18913 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504616.1 12 32-54,69-91,103-125,129-151,164-186,201-223,243-265,289-311,324-343,374-396,417-439,444-466
T28C12.2 12 32-54,69-91,103-125,129-151,164-186,201-223,243-265,289-311,324-343,374-396,417-439,444-466
SNAP00000017147 12 32-54,69-91,103-125,129-151,164-186,201-223,243-265,289-311,324-343,374-396,417-439,444-466
GENEFINDER00000017158 12 32-54,69-91,103-125,129-151,164-186,201-223,243-265,289-311,324-343,374-396,417-439,444-466
Sequence
Target Sequence 
>C. ELEGANS:185917_AT
gttgtaccaggcattcatgtatccatggggattctactcgggaccactgaacttcttgcc
tccaggtgttgaaactaccgaagtgggaggatgcttggtagaatatcattggtgtcagga
aactaccagggtcccactgtccgtgtatcttttctgttttattgtattctttggagttgc
atttccatttgtggagacaccgtccgcggcactttattcggaagttcttggtccaaggaa
acaaggcacgatgcagggatttttctcactcggtggaagtattgctccagttattgcttc
tatttcttcaacagcgatcttcaaatatacgggttatcgctacgttattgtacttcaatc
agtatttcttctcgttggagcggcactcattttaatatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGTACCAGGCATTCATGTATCCA 560 435 1008 Antisense
AACTTCTTGCCTCCAGGTGTTGAAA 622 137 1057 Antisense
GTCAGGAAACTACCAGGGTCCCACT 201 457 1121 Antisense
TCCGTGTATCTTTTCTGTTTTATTG 269 595 1147 Antisense
CGTCCGCGGCACTTTATTCGGAAGT 256 243 1208 Antisense
GGCACGATGCAGGGATTTTTCTCAC 634 535 1252 Antisense
GGAAGTATTGCTCCAGTTATTGCTT 392 531 1282 Antisense
TCTATTTCTTCAACAGCGATCTTCA 376 615 1306 Antisense
GGTTATCGCTACGTTATTGTACTTC 206 505 1339 Antisense
GTATTTCTTCTCGTTGGAGCGGCAC 316 453 1369 Antisense
GGAGCGGCACTCATTTTAATATTTT 701 521 1384 Antisense
Sequence Source Affymetrix Proprietary Database