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[185915_at]

Description Y51A2D.19 /REP_DB=WormBase Gene ID /WP=CE24409 /TR=Q9U268 /GB=CAB54459.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 70      6grp_total > 15      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 537.2
(3)
P 		484.9
(2)
P 		257.7
(-2.4)
P 		226.3
(-3)
P 		311
B 469.7
(3)
P 		355.8
(-0.1)
P 		302.2
(-1.6)
P 		250
(-3)
P 		220
C 479.7
(3)
P 		437.9
(2)
P 		239.1
(-3)
P 		255.4
(-2.6)
P 		241
avr 495.5
(3) 		426.2
(1.3) 		266.3
(-2.5) 		243.9
(-3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 459
1 2 3 4 5 6 range
A 320
(0.9)
P 		406.3
(3)
P 		217.6
(-1.5)
P 		202
(-1.9)
P 		201
(-1.9)
P 		157
(-3)
P 		249
B 385.5
(2.7)
P 		402.3
(3)
P 		281.4
(0.6)
P 		225.6
(-0.5)
P 		210.2
(-0.9)
P 		103.1
(-3)
P 		299
C 561.6
(3)
P 		385.5
(0.6)
P 		283.1
(-0.8)
P 		195.1
(-2)
P 		210.6
(-1.8)
P 		120.9
(-3)
P 		441
avr 422.4
(3) 		398
(2.5) 		260.7
(-0.3) 		207.6
(-1.4) 		207.3
(-1.4) 		127
(-3) 		295
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 163
1 2 3 4 range
A 195.6
(3)
P 		105.3
(-0.3)
P 		67.4
(-1.7)
P 		33.1
(-3)
P 		163
B 149.8
(3)
P 		87.9
(-0.8)
P 		70
(-2)
P 		53.3
(-3)
P 		97
C 158.8
(3)
P 		125.9
(1.3)
P 		83.3
(-0.9)
P 		43.1
(-3)
P 		116
avr 168.1
(3) 		106.4
(0) 		73.6
(-1.5) 		43.2
(-3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 59
(-1.8)
P 		144.5
(3)
P 		47.4
(-2.5)
P 		51.2
(-2.3)
P 		60.7
(-1.7)
P 		38.2
(-3)
P 		106
B 134.1
(3)
P 		133.9
(3)
P 		68.3
(-1.1)
P 		59.8
(-1.7)
P 		85.4
(-0.1)
P 		38.4
(-3)
P 		96
C 208.1
(3)
P 		117.6
(-0.2)
P 		72.1
(-1.8)
P 		39.2
(-3)
P 		68.2
(-2)
P 		40.5
(-3)
P 		169
avr 133.7
(3) 		132
(2.9) 		62.6
(-1.5) 		50.1
(-2.3) 		71.4
(-0.9) 		39
(-3) 		95
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185915_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14279
Sequence Type Exemplar sequence
Representative Public ID Y51A2D.19 NCBI
Target Description Y51A2D.19 /REP_DB=WormBase Gene ID /WP=CE24409 /TR=Q9U268 /GB=CAB54459.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029090(11), NM_001029088(11), NM_001029089(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029090 NCBI Caenorhabditis elegans SLOwpoke potassium channel family member (slo-1) (slo-1) mRNA, complete cds. 11/11 None
NM_001029088 NCBI Caenorhabditis elegans SLOwpoke potassium channel family member (slo-1) (slo-1) mRNA, complete cds. 11/11 None
NM_001029089 NCBI Caenorhabditis elegans SLOwpoke potassium channel family member (slo-1) (slo-1) mRNA, complete cds. 11/11 None
GENEFINDER00000034270 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18493081:18510508:1 11/11 None
Y51A2D.19b ENSEMBL cdna:known chromosome:CEL140:V:18494880:18510508:1 gene:Y51A2D.19 11/11 None
Y51A2D.19a ENSEMBL cdna:known chromosome:CEL140:V:18494880:18510508:1 gene:Y51A2D.19 11/11 None
Y51A2D.19c ENSEMBL cdna:known chromosome:CEL140:V:18494880:18510711:1 gene:Y51A2D.19 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034243 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18497440-18510508(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y51A2D.19
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD27618 Hypothetical protein Y51A2D.19c [Caenorhabditis elegans] ref|NP_001024261.1| SLOwpoke potassium channel family member (slo-1) [Caenorhabditis elegans] gb|AAL28104.1| large-conductance calcium-activated potassium channel SLO-1c [Caenorhabditis elegans] 0.0
blast CAD27617 Hypothetical protein Y51A2D.19b [Caenorhabditis elegans] ref|NP_001024260.1| SLOwpoke potassium channel family member (slo-1) [Caenorhabditis elegans] gb|AAL28103.1| large-conductance calcium-activated potassium channel SLO-1b [Caenorhabditis elegans] 0.0
blast CAB54459 Hypothetical protein Y51A2D.19a [Caenorhabditis elegans] ref|NP_001024259.1| SLOwpoke potassium channel family member (slo-1) [Caenorhabditis elegans] gb|AAL28102.1| large-conductance calcium-activated potassium channel SLO-1a [Caenorhabditis elegans] sp|Q95V25|SLO1_CAEEL Calcium-activated potassium channel slo-1 (Maxi K channel) (MaxiK) (BK channel) (Slo homolog) (Slowpoke protein 1) 0.0
blast CAE67433 Hypothetical protein CBG12923 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 7.3E-25
Pfam IPR003929 EMBL-EBI Calcium-activated BK potassium channel, alpha subunit 1.0E-126
Pfam IPR003929 EMBL-EBI Calcium-activated BK potassium channel, alpha subunit 1.0E-126
Pfam IPR003929 EMBL-EBI Calcium-activated BK potassium channel, alpha subunit 1.0E-126
Pfam IPR003929 EMBL-EBI Calcium-activated BK potassium channel, alpha subunit 1.0E-126
Pfam IPR005821 EMBL-EBI Ion transport protein 7.3E-25
Pfam IPR003929 EMBL-EBI Calcium-activated BK potassium channel, alpha subunit 2.0E-94
Pfam IPR003929 EMBL-EBI Calcium-activated BK potassium channel, alpha subunit 3.7E-38
Pfam IPR003929 EMBL-EBI Calcium-activated BK potassium channel, alpha subunit 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024261.1 7 47-69,140-162,183-200,205-227,264-286,301-320,327-349
NP_001024259.1 7 47-69,140-162,183-200,205-227,264-286,301-320,327-349
NP_001024260.1 7 47-69,140-162,183-200,205-227,264-286,301-320,327-349
Y51A2D.19b 7 47-69,140-162,183-200,205-227,264-286,301-320,327-349
Y51A2D.19a 7 47-69,140-162,183-200,205-227,264-286,301-320,327-349
Y51A2D.19c 7 47-69,140-162,183-200,205-227,264-286,301-320,327-349
GENEFINDER00000034270 7 136-158,229-251,272-289,294-316,353-375,390-409,416-438
Sequence
Target Sequence 
>C. ELEGANS:185915_AT
gtttgggaccaatgtgccgatgattacggagcttgttaacgactcaaatgtgcaattcct
cgaccaggacgacgacgacgatccggacaccgagctctacctcacgcagcccttcgcctg
cggaactgccttcgccatttcagtgttggattctttgatgagtactacctacttcaacga
ctcggccctgaccctcatccgaactctggtcactggtggagccacaccggagcttgaact
tattctggcagaaggcgccgggctccgaggtggctacagtaccccggagaccctctcaaa
cagagatcggtgccgaattgctcagatttcgctgcaggataacccgtacgatggagttgt
gcacaacactacctatggtgcaatgttcacgatagctctgaggaggtacggccaactttg
tatcgggctgtataggcttcacgaccaggataatccggactctatgaagcgatatgttat
cactaatcctccagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTGGGACCAATGTGCCGATGATT 606 445 2385 Antisense
GACTCAAATGTGCAATTCCTCGACC 412 373 2425 Antisense
ACCAGGACGACGACGACGATCCGGA 366 85 2447 Antisense
GAGTACTACCTACTTCAACGACTCG 245 391 2544 Antisense
CATCCGAACTCTGGTCACTGGTGGA 204 211 2580 Antisense
TCCGAGGTGGCTACAGTACCCCGGA 549 593 2648 Antisense
GATCGGTGCCGAATTGCTCAGATTT 229 419 2689 Antisense
GATTTCGCTGCAGGATAACCCGTAC 688 427 2709 Antisense
GGTACGGCCAACTTTGTATCGGGCT 615 503 2789 Antisense
CGGGCTGTATAGGCTTCACGACCAG 611 243 2808 Antisense
ATATGTTATCACTAATCCTCCAGCA 450 21 2856 Antisense
Sequence Source Affymetrix Proprietary Database