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[185914_at]

Description Y48C3A.20 /REP_DB=WormBase Gene ID /WP=CE24383 /TR=Q9U287 /GB=CAB60423.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 75      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 93      6grp_total > 76      4grp_total > 55      6grp_highest_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 380
1 2 3 4 range
A 877.2
(3)
P 		560.2
(-2)
P 		682.9
(-0.1)
P 		496.8
(-3)
P 		380
B 719.5
(1.1)
P 		787
(3)
P 		570.2
(-3)
P 		685
(0.2)
P 		217
C 796.6
(2.7)
P 		542.6
(-3)
P 		807.8
(3)
P 		611.7
(-1.4)
P 		265
avr 797.8
(3) 		629.9
(-2) 		687
(-0.3) 		597.8
(-3) 		200

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 773
1 2 3 4 5 6 range
A 193.4
(-3)
P 		300
(-1.8)
P 		409.8
(-0.5)
P 		360
(-1)
P 		259.3
(-2.2)
P 		705.7
(3)
P 		512
B 245.3
(-2.2)
P 		143.1
(-3)
P 		351.9
(-1.3)
P 		350.7
(-1.3)
P 		209.8
(-2.5)
P 		871.7
(3)
P 		729
C 98.9
(-3)
P 		158.8
(-2.5)
P 		492.1
(0.2)
P 		342.3
(-1)
P 		262.1
(-1.7)
P 		831
(3)
P 		732
avr 179.2
(-3) 		200.6
(-2.8) 		417.9
(-0.7) 		351
(-1.3) 		243.7
(-2.4) 		802.8
(3) 		624
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 247
1 2 3 4 range
A 319.4
(3)
P 		121.6
(-1.8)
P 		178.5
(-0.4)
P 		72.7
(-3)
P 		247
B 229.5
(3)
P 		194.4
(0.8)
P 		132.1
(-3)
P 		146
(-2.1)
P 		97
C 263.7
(2.4)
P 		156
(-1.2)
P 		281.6
(3)
P 		103.1
(-3)
P 		179
avr 270.9
(3) 		157.3
(-1.2) 		197.4
(0.3) 		107.3
(-3) 		164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 289
1 2 3 4 5 6 range
A 35.7
(-3)
P 		106.7
(0.1)
P 		89.2
(-0.6)
P 		91.3
(-0.5)
P 		78.2
(-1.1)
P 		171.8
(3)
P 		136
B 85.4
(-2.2)
P 		47.6
(-3)
P 		85.5
(-2.2)
P 		92.9
(-2)
P 		85.2
(-2.2)
P 		325.1
(3)
P 		278
C 36.6
(-3)
P 		48.5
(-2.7)
P 		125.3
(-0.8)
P 		68.8
(-2.2)
P 		84.8
(-1.8)
P 		278.2
(3)
P 		242
avr 52.6
(-3) 		67.6
(-2.6) 		100
(-1.6) 		84.3
(-2.1) 		82.7
(-2.1) 		258.4
(3) 		206
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185914_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2809
Sequence Type Exemplar sequence
Representative Public ID Y48C3A.20 NCBI
Target Description Y48C3A.20 /REP_DB=WormBase Gene ID /WP=CE24383 /TR=Q9U287 /GB=CAB60423.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064418(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064418 NCBI Caenorhabditis elegans Y48C3A.20 (Y48C3A.20) mRNA, complete cds. 11/11 None
GENEFINDER00000006658 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13369503:13383806:1 11/11 None
Y48C3A.20 ENSEMBL cdna:known chromosome:CEL140:II:13369503:13375186:1 gene:Y48C3A.20 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13369499-13375183(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y48C3A.20
Entrez Gene 174977 Entrez gene
SwissProt Q9U287 EMBL-EBI
WormBase CE24383 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259571_AT expressed protein at
CANINE_2:CFAAFFX.5413.1.S1_AT similar to CG3709-PA cfa
DROSOPHILA_2:1638095_AT dm
DROSGENOME1:145515_AT dm
CHICKEN:GGAAFFX.4897.1.S1_AT similar to hypothetical protein FLJ32312 gga
U133_X3P:HS2.112830.1.S1_3P_AT hypothetical protein FLJ32312 hs
HG-U133_PLUS_2:1553280_AT hypothetical protein FLJ32312 hs
MG-U74BV2:162772_F_AT RIKEN cDNA 4933435A13 gene mm
MG-U74CV2:167170_AT RIKEN cDNA 4933435A13 gene mm
MOE430A:1452075_AT RIKEN cDNA 4933435A13 gene mm
MOUSE430A_2:1452075_AT RIKEN cDNA 4933435A13 gene mm
MOUSE430_2:1452075_AT RIKEN cDNA 4933435A13 gene mm
MOUSE430_2:1445784_AT RIKEN cDNA 4933435A13 gene (4933435A13Rik), transcript variant 1, mRNA mm
MOE430B:1445784_AT RIKEN cDNA 4933435A13 gene (4933435A13Rik), transcript variant 1, mRNA mm
MU19KSUBC:TC35080_AT RIKEN cDNA 4933435A13 gene (4933435A13Rik), transcript variant 1, mRNA mm
MU19KSUBC:TC35080_G_AT RIKEN cDNA 4933435A13 gene (4933435A13Rik), transcript variant 1, mRNA mm
Protein Similarities
Method ID Description E-Value
blast CAB60423 Hypothetical protein Y48C3A.20 [Caenorhabditis elegans] ref|NP_496819.1| Y48C3A.20 [Caenorhabditis elegans] 0.0
blast CAE73359 Hypothetical protein CBG20791 [Caenorhabditis briggsae] emb|CAE73356.1| Hypothetical protein CBG20788 [Caenorhabditis briggsae] 0.0
blast T31596 hypothetical protein Y48C3A.n - Caenorhabditis elegans 0.0
blast T31597 hypothetical protein Y48C3A.o - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000580 EMBL-EBI TSC-22 / Dip / Bun 6.1E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000006658 2 597-619,626-648
Sequence
Target Sequence 
>C. ELEGANS:185914_AT
cccatttgtcgttgagctccgaaattgtagaacgacggctccagtgagaggaatcaagta
tttggagacactgaagacggttcaggaggctataaatgtacagaaggatgtgaaagtcgt
gtcgctgaccagagttccacgggatgtttctgagaagctgtgtgttggagaagaagacaa
acgaaagcaatattgtgcctactgctacagtattctcccaatttctgactcccaattcac
cgctgctcaatcgaaaatcccgctgaaaattgttcaaaagacgccagttcgtgtgatgaa
gcgtcgagctctactcgacagagaacgatggatttattcgatgaatttcttaaaattgga
cgatcaccactttgaagttcgtctcgagacccaagcaggaacatatatcaaggaatttgt
tcacggagattttggaaggacgaggccttcgcttgccgagctccttgacgttgagcacgg
agaagttgatattttggagctggatgtggagaaagttgatttggaatggccaccggagct
taaggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCATTTGTCGTTGAGCTCCGAAAT 100 259 849 Antisense
GAAAGTCGTGTCGCTGACCAGAGTT 662 359 960 Antisense
GACCAGAGTTCCACGGGATGTTTCT 328 371 975 Antisense
CAATATTGTGCCTACTGCTACAGTA 72 181 1036 Antisense
ACAGTATTCTCCCAATTTCTGACTC 337 107 1055 Antisense
TCACCGCTGCTCAATCGAAAATCCC 442 625 1085 Antisense
GATCACCACTTTGAAGTTCGTCTCG 473 421 1210 Antisense
TCGTCTCGAGACCCAAGCAGGAACA 309 605 1227 Antisense
TGGAAGGACGAGGCCTTCGCTTGCC 426 545 1281 Antisense
TGCCGAGCTCCTTGACGTTGAGCAC 91 585 1302 Antisense
GGAATGGCCACCGGAGCTTAAGGAT 322 529 1371 Antisense
Sequence Source Affymetrix Proprietary Database