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[185912_at]

Description T20D4.13 /REP_DB=WormBase Gene ID /WP=CE13804 /TR=P91459 /GB=AAB37581.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 42      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 6.1
(-3)
A 		36.4
(-0)
A 		8.1
(-2.8)
A 		67.6
(3)
A 		62
B 4.3
(-3)
A 		27.9
(2.8)
A 		4.8
(-2.9)
A 		28.9
(3)
A 		25
C 5.5
(-2.8)
A 		14.2
(-2.1)
A 		2.5
(-3)
A 		80.6
(3)
A 		78
avr 5.3
(-3) 		26.2
(-0.7) 		5.1
(-3) 		59
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 5.6
(-2.3)
A 		4.2
(-2.8)
A 		19.4
(3)
A 		5.7
(-2.2)
A 		3.7
(-3)
A 		4.5
(-2.7)
A 		16
B 8
(3)
A 		6.4
(1.4)
A 		6.9
(1.9)
A 		4.9
(-0.1)
A 		6.5
(1.5)
A 		2
(-3)
A 		6
C 5.4
(-1.2)
A 		10
(3)
A 		3.6
(-2.9)
A 		7.3
(0.5)
A 		8.9
(2)
A 		3.5
(-3)
A 		7
avr 6.3
(-0.3) 		6.9
(0.2) 		10
(3) 		6
(-0.6) 		6.4
(-0.2) 		3.3
(-3) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 2.2
(-2.9)
A 		7.9
(1.5)
A 		2.1
(-3)
A 		9.9
(3)
A 		8
B 1.4
(-2.7)
A 		6.9
(3)
A 		1.1
(-3)
A 		6.2
(2.3)
A 		6
C 1.8
(-2.6)
A 		4.1
(-1.5)
A 		0.9
(-3)
A 		13.6
(3)
A 		13
avr 1.8
(-2.7) 		6.3
(0.5) 		1.4
(-3) 		9.9
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 1
(-3)
A 		1.5
(-2.1)
A 		4.2
(3)
A 		1.5
(-2.1)
A 		1.1
(-2.8)
A 		1.1
(-2.8)
A 		3
B 2.8
(3)
A 		2.1
(1)
A 		1.7
(-0.1)
A 		1.3
(-1.3)
A 		2.6
(2.4)
A 		0.7
(-3)
A 		2
C 2
(0)
A 		3.1
(3)
A 		0.9
(-3)
A 		1.5
(-1.4)
A 		2.9
(2.5)
A 		1.2
(-2.2)
A 		2
avr 1.9
(1.2) 		2.2
(2.5) 		2.3
(3) 		1.4
(-1.2) 		2.2
(2.5) 		1
(-3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185912_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15191
Sequence Type Exemplar sequence
Representative Public ID T20D4.13 NCBI
Target Description T20D4.13 /REP_DB=WormBase Gene ID /WP=CE13804 /TR=P91459 /GB=AAB37581.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071544(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071544 NCBI Caenorhabditis elegans T20D4.13 (T20D4.13) mRNA, complete cds. 11/11 None
GENEFINDER00000008868 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3391937:3393266:1 11/11 None
T20D4.13 ENSEMBL cdna:known chromosome:CEL140:V:3391937:3393266:1 gene:T20D4.13 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000008841 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3391936-3393266(+) 100.0 100.0
chrV:3316210-3317725(+) 90.48 90.48
Public Domain and Genome References
Gene Title
Ensembl T20D4.13
Entrez Gene 188649 Entrez gene
SwissProt P91459 EMBL-EBI
WormBase CE13804 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99930 Hypothetical protein F35F10.1 [Caenorhabditis elegans] ref|NP_503918.2| F35F10.1 [Caenorhabditis elegans] 1.0E-127
blast AAC27089 Hypothetical protein C04E12.4 [Caenorhabditis elegans] ref|NP_503936.2| C04E12.4 [Caenorhabditis elegans] 1.0E-102
blast T33413 hypothetical protein C04E12.4 - Caenorhabditis elegans 3.0E-98
blast AAB37581 Hypothetical protein T20D4.13 [Caenorhabditis elegans] ref|NP_503945.1| T20D4.13 [Caenorhabditis elegans] 4.0E-89
blast AAF99930 Hypothetical protein F35F10.1 [Caenorhabditis elegans] ref|NP_503918.2| F35F10.1 [Caenorhabditis elegans] 3.0E-56
blast AAC27089 Hypothetical protein C04E12.4 [Caenorhabditis elegans] ref|NP_503936.2| C04E12.4 [Caenorhabditis elegans] 3.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.2E-10
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 3.3E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503945.1 2 31-53,141-163
T20D4.13 2 31-53,141-163
GENEFINDER00000008868 2 31-53,250-272
Sequence
Target Sequence 
>C. ELEGANS:185912_AT
gtcttctcaaaatcccacaaatgcacaatactctgctctgtctttaggtggtgaaccaat
cagatcgcaaccaagtcatcctggcaagagcatcgcaaaaataactaccgctttcattgg
agctcttgtagtttcagcaacaattctcattgccttgtcggaaactactacttcttccca
tcttgcagcaccaagtcatgaggttttagcaacacaggacgagcctatccaattggaaaa
tgactcacaggacaaatacgtagagtttcggtatgatccaatatccggaaagtactccca
caacgttgagcggcactctgaaaaacaatacgctgtctacttacagaagaaagataaaaa
tgaagaaggaagtccatctgaattcgcaattcatattttatttttaaaacttgtctcaat
aaatcaaattactctaataaacataatatttcgtcttcttgcgtattatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTTCTCAAAATCCCACAAATGCA 492 467 15 Antisense
CTCTGCTCTGTCTTTAGGTGGTGAA 628 231 45 Antisense
GGTGGTGAACCAATCAGATCGCAAC 519 499 61 Antisense
ATCAGATCGCAACCAAGTCATCCTG 378 29 73 Antisense
GTCATCCTGGCAAGAGCATCGCAAA 532 467 89 Antisense
CATTGCCTTGTCGGAAACTACTACT 105 217 162 Antisense
TCGGAAACTACTACTTCTTCCCATC 213 607 172 Antisense
TATCCGGAAAGTACTCCCACAACGT 508 655 296 Antisense
CCACAACGTTGAGCGGCACTCTGAA 179 275 312 Antisense
GGAAGTCCATCTGAATTCGCAATTC 152 533 382 Antisense
ATATTTCGTCTTCTTGCGTATTATG 400 19 460 Antisense
Sequence Source Affymetrix Proprietary Database