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[185906_at]

Description H12I19.5C /REP_DB=WormBase Gene ID /WP=CE16179 /TR=O45619 /GB=CAB11540.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 83      4grp_total > 32      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 3.6
(-3)
A 		38.1
(3)
A 		35.6
(2.6)
A 		18.5
(-0.4)
A 		35
B 6.6
(-0.7)
A 		2
(-3)
A 		12.9
(2.4)
A 		14.1
(3)
A 		12
C 24.9
(1.5)
A 		2.2
(-3)
A 		17.9
(0.1)
A 		32.7
(3)
A 		31
avr 11.7
(-3) 		14.1
(-1.6) 		22.1
(3) 		21.8
(2.8) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 7.2
(-1.9)
A 		1.6
(-3)
A 		31.5
(2.6)
A 		3.6
(-2.6)
A 		31.6
(2.6)
A 		33.5
(3)
A 		32
B 4
(-2.7)
A 		2
(-3)
A 		18.3
(-0.6)
A 		24.3
(0.2)
A 		43.6
(3)
A 		13.8
(-1.3)
A 		42
C 2.5
(-2.9)
A 		45.9
(2.1)
M 		1.7
(-3)
A 		54
(3)
P 		29.6
(0.2)
M 		18.4
(-1.1)
M 		52
avr 4.6
(-3) 		16.5
(-0.6) 		17.2
(-0.5) 		27.3
(1.5) 		34.9
(3) 		21.9
(0.4) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.3
(-3)
A 		8.3
(2.3)
A 		9.3
(3)
A 		2.7
(-2)
A 		8
B 2.1
(0.8)
A 		0.5
(-3)
A 		3
(3)
A 		3
(3)
A 		3
C 8.2
(3)
A 		0.6
(-3)
A 		6.2
(1.4)
A 		5.5
(0.9)
A 		8
avr 3.9
(-1.5) 		3.1
(-3) 		6.2
(3) 		3.7
(-1.8) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 1.3
(-2.5)
A 		0.6
(-3)
A 		6.9
(1.2)
A 		0.9
(-2.8)
A 		9.5
(3)
A 		8.2
(2.1)
A 		9
B 1.4
(-2.8)
A 		0.7
(-3)
A 		4.4
(-1.7)
A 		6.4
(-1)
A 		17.7
(3)
A 		5.1
(-1.4)
A 		17
C 0.9
(-2.8)
A 		14
(3)
M 		0.4
(-3)
A 		10.9
(1.6)
P 		9.6
(1.1)
M 		6.1
(-0.5)
M 		14
avr 1.2
(-3) 		5.1
(-0.9) 		3.9
(-1.5) 		6.1
(-0.4) 		12.3
(3) 		6.5
(-0.1) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185906_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9280
Sequence Type Exemplar sequence
Representative Public ID H12I19.5C NCBI
Target Description H12I19.5C /REP_DB=WormBase Gene ID /WP=CE16179 /TR=O45619 /GB=CAB11540.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070311(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070311 NCBI Caenorhabditis elegans H12I19.5b (H12I19.5) mRNA, complete cds. 10/11 None
H12I19.5c ENSEMBL cdna:known chromosome:CEL140:IV:14150261:14153296:-1 gene:H12I19.5 11/11 A 
H12I19.5b ENSEMBL cdna:known chromosome:CEL140:IV:14150290:14153296:-1 gene:H12I19.5 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070312 8/11 Cross Hyb Matching Probes None
H12I19.5a 8/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178369 2 1 NM_070311 185906_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14150266-14153302(-) 100.0 100.0
Public Domain and Genome References
Ensembl H12I19.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB11541 Hypothetical protein H12I19.5b [Caenorhabditis elegans] ref|NP_502712.1| H12I19.5b [Caenorhabditis elegans] 0.0
blast CAB11542 Hypothetical protein H12I19.5a [Caenorhabditis elegans] ref|NP_502713.1| H12I19.5a [Caenorhabditis elegans] 0.0
blast CAE61778 Hypothetical protein CBG05738 [Caenorhabditis briggsae] 1.0E-176
blast CAE61778 Hypothetical protein CBG05738 [Caenorhabditis briggsae] 1.0E-151
Protein Domains
Database ID Description E-Value
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502712.1 7 28-50,77-96,139-161,168-187,244-266,287-309,319-341
H12I19.5c 8 28-50,77-96,139-161,168-187,244-266,287-309,319-341,353-370
H12I19.5b 7 28-50,77-96,139-161,168-187,244-266,287-309,319-341
Sequence
Target Sequence 
>C. ELEGANS:185906_AT
gatgtgagagttttttcaagcctaaagttgttgaaacagtgttcaaaatgtgaacttatc
gattacaaaccgtttttctggagaaatgccacccaaacatggcgattctatgacgtagac
tataatggactgagctatttcacatctgaaaatcattttagttttcacggtctcgaacta
attcgcagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTGAGAGTTTTTTCAAGCCTAA 563 407 1828 Antisense
GTGAACTTATCGATTACAAACCGTT 277 479 1877 Antisense
TCGATTACAAACCGTTTTTCTGGAG 174 603 1886 Antisense
TTTCTGGAGAAATGCCACCCAAACA 352 669 1902 Antisense
AATGCCACCCAAACATGGCGATTCT 160 167 1912 Antisense
ACATGGCGATTCTATGACGTAGACT 531 105 1924 Antisense
GACGTAGACTATAATGGACTGAGCT 104 379 1939 Antisense
TGGACTGAGCTATTTCACATCTGAA 67 549 1953 Antisense
ATCATTTTAGTTTTCACGGTCTCGA 78 31 1979 Antisense
GTTTTCACGGTCTCGAACTAATTCG 125 447 1988 Antisense
TCACGGTCTCGAACTAATTCGCAGT 382 625 1992 Antisense
Sequence Source Affymetrix Proprietary Database