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[185905_at]

Description T25F10.4 /REP_DB=WormBase Gene ID /WP=CE07535 /TR=Q23048 /GB=AAB04987.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 05      6grp_total > 45      4grp_total > 04      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 64.8
(-3)
P 		86.1
(-2.2)
P 		79.9
(-2.4)
A 		216.5
(3)
P 		152
B 105.1
(0.7)
P 		33.2
(-3)
P 		61.8
(-1.5)
A 		149.2
(3)
P 		116
C 110.8
(0.6)
P 		113.4
(0.8)
P 		73.3
(-3)
P 		136
(3)
P 		63
avr 93.6
(-1.6) 		77.6
(-2.6) 		71.7
(-3) 		167.2
(3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 58.9
(-1.4)
P 		62.3
(-1.1)
A 		91.7
(1.3)
P 		40.1
(-3)
A 		111.5
(3)
P 		84.9
(0.8)
A 		71
B 97.2
(3)
P 		95.8
(2.8)
P 		77.9
(0.6)
A 		64.2
(-1.1)
P 		60.1
(-1.6)
P 		49
(-3)
A 		48
C 97.5
(1.9)
P 		107.3
(3)
P 		89.2
(1.1)
A 		58.3
(-2.3)
P 		51.4
(-3)
A 		99.8
(2.2)
M 		56
avr 84.5
(2.3) 		88.5
(3) 		86.3
(2.6) 		54.2
(-3) 		74.3
(0.5) 		77.9
(1.1) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 23.6
(-0.7)
P 		18.7
(-3)
P 		20.9
(-2)
A 		31.7
(3)
P 		13
B 33.5
(3)
P 		8.2
(-3)
P 		14.3
(-1.6)
A 		31.8
(2.6)
P 		25
C 36.7
(3)
P 		32.6
(1.2)
P 		25.6
(-1.8)
P 		22.9
(-3)
P 		14
avr 31.3
(3) 		19.8
(-3) 		20.3
(-2.7) 		28.8
(1.7) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 10.9
(-2.8)
P 		22.2
(0.1)
A 		20
(-0.5)
P 		10.2
(-3)
A 		33.6
(3)
P 		20.7
(-0.3)
A 		23
B 33.8
(3)
P 		31.9
(2.3)
P 		18.9
(-2.3)
A 		17
(-3)
P 		24.4
(-0.4)
P 		18.3
(-2.5)
A 		17
C 36.2
(3)
P 		32.8
(2.2)
P 		22.7
(-0.3)
A 		11.7
(-3)
P 		16.6
(-1.8)
A 		33.4
(2.3)
M 		25
avr 27
(2.3) 		29
(3) 		20.5
(-0.2) 		13
(-3) 		24.9
(1.5) 		24.1
(1.2) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185905_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11701
Sequence Type Exemplar sequence
Representative Public ID T25F10.4 NCBI
Target Description T25F10.4 /REP_DB=WormBase Gene ID /WP=CE07535 /TR=Q23048 /GB=AAB04987.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072309(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072309 NCBI Caenorhabditis elegans T25F10.4 (T25F10.4) mRNA, complete cds. 11/11 None
SNAP00000017679 ENSEMBL cdna:SNAP chromosome:CEL140:V:6763100:6764541:-1 9/11 None
GENEFINDER00000017690 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6763100:6765027:-1 10/11 None
T25F10.4 ENSEMBL cdna:known chromosome:CEL140:V:6763036:6765027:-1 gene:T25F10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6763036-6765028(-) 99.58 100.0
Public Domain and Genome References
Gene Title
Ensembl T25F10.4
Entrez Gene 188903 Entrez gene
SwissProt Q23048 EMBL-EBI
WormBase CE07535 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04987 Hypothetical protein T25F10.4 [Caenorhabditis elegans] ref|NP_504710.1| T25F10.4 [Caenorhabditis elegans] 0.0
blast AAB04987 Hypothetical protein T25F10.4 [Caenorhabditis elegans] ref|NP_504710.1| T25F10.4 [Caenorhabditis elegans] 1.0E-119
blast CAE71945 Hypothetical protein CBG19012 [Caenorhabditis briggsae] 2.0E-71
blast CAE71945 Hypothetical protein CBG19012 [Caenorhabditis briggsae] 1.0E-57
blast CAE71945 Hypothetical protein CBG19012 [Caenorhabditis briggsae] 5.0E-43
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504710.1 4 199-218,225-244,259-281,371-390
T25F10.4 4 199-218,225-244,259-281,371-390
SNAP00000017679 2 85-107,122-144
GENEFINDER00000017690 3 199-218,225-244,259-281
Sequence
Target Sequence 
>C. ELEGANS:185905_AT
ttgagttccgtgttactcgacgtccaccgactttccaacgaattggaatgttcttattat
tccttttgaatgtttccatgatatttgaatacttcccagtttccagaatattttggaatt
tgccatcgtcgagattctacctatgttgtggttttcttatcagtctagtatcagcaagtc
aaggcccaaaatctgggaaattgacaagttttgattatcaactaatgacactgcttttac
tggaggttttcattgaaattcttcaccaactacttccgttttttatttttcacttgaggt
gctgtccgatcgaatctgatccaactccaccactatttccatcgccaagtgatcaattta
gtctagatacagagagatcaaaatgtaatgctgcattggattctgcaagagccgagtcta
tgcgaagtccgagaattgcaaactttgatatgtgcttggctgaagtaaaatttacaatgt
ctacattcaatgaagttctgaatttgcattcttctgcagaatggagacgaaagctgtggc
taaacgcctaccgtcggtttgaaattcttgcgttcatagttctcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAGTTCCGTGTTACTCGACGTCC 140 665 566 Antisense
GTCCACCGACTTTCCAACGAATTGG 549 473 587 Antisense
GCCATCGTCGAGATTCTACCTATGT 182 281 687 Antisense
GTTGTGGTTTTCTTATCAGTCTAGT 374 435 710 Antisense
GACACTGCTTTTACTGGAGGTTTTC 548 365 792 Antisense
TGAAATTCTTCACCAACTACTTCCG 482 571 819 Antisense
TGAGGTGCTGTCCGATCGAATCTGA 457 567 860 Antisense
CACCACTATTTCCATCGCCAAGTGA 100 203 893 Antisense
AAGAGCCGAGTCTATGCGAAGTCCG 5 153 972 Antisense
AAAGCTGTGGCTAAACGCCTACCGT 248 121 1095 Antisense
AAATTCTTGCGTTCATAGTTCTCCA 90 117 1127 Antisense
Sequence Source Affymetrix Proprietary Database