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[185898_at]

Description C28C12.7 /REP_DB=WormBase Gene ID /WP=CE04114 /TR=Q18276 /GB=AAB37550.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 61      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 27      6grp_total > 71      4grp_total > 58      6grp_highest_sub > 04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2227
1 2 3 4 range
A 5489.3
(-0.1)
P 		6647.1
(3)
P 		6439.3
(2.4)
P 		4420.6
(-3)
P 		2227
B 5780.8
(0.8)
P 		6002.1
(3)
P 		5886.5
(1.9)
P 		5394.9
(-3)
P 		607
C 5496.9
(-1.5)
P 		5503.5
(-1.4)
P 		6277.7
(3)
P 		5228.7
(-3)
P 		1049
avr 5589
(-0.1) 		6050.9
(2.2) 		6201.2
(3) 		5014.7
(-3) 		1187

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4357
1 2 3 4 5 6 range
A 5875.9
(-1.1)
P 		6290.6
(-0.4)
P 		7306.2
(1.2)
P 		8381.2
(3)
P 		7573.1
(1.7)
P 		4703.2
(-3)
P 		3678
B 6234.2
(-0.7)
P 		6528.3
(-0.3)
P 		8415.5
(2.3)
P 		8948.4
(3)
P 		6103
(-0.9)
P 		4591.9
(-3)
P 		4357
C 5435
(-1.7)
P 		5799.2
(-1.1)
P 		7836.5
(2.3)
P 		8267
(3)
P 		6645.6
(0.3)
P 		4652.9
(-3)
P 		3614
avr 5848.4
(-1.1) 		6206
(-0.6) 		7852.7
(2) 		8532.2
(3) 		6773.9
(0.3) 		4649.3
(-3) 		3883
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1541
1 2 3 4 range
A 1998.7
(3)
P 		1442.9
(0.5)
P 		1683.3
(1.6)
P 		647.1
(-3)
P 		1352
B 1843.4
(3)
P 		1482.4
(-0.1)
P 		1364
(-1.1)
P 		1149.7
(-3)
P 		694
C 1819.6
(1.3)
P 		1582.3
(0.2)
P 		2188
(3)
P 		881.6
(-3)
P 		1306
avr 1887.2
(3) 		1502.5
(0.7) 		1745.1
(2.1) 		892.8
(-3) 		994

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1396
1 2 3 4 5 6 range
A 1083.2
(-3)
P 		2237.3
(2.8)
P 		1591.1
(-0.5)
P 		2125.7
(2.2)
P 		2285.2
(3)
P 		1145.1
(-2.7)
P 		1202
B 2169.2
(0.6)
P 		2173
(0.6)
P 		2043.5
(-0.4)
P 		2370.1
(2.1)
P 		2479.6
(3)
P 		1712.3
(-3)
P 		767
C 2014.5
(1.6)
P 		1769.5
(-0.9)
P 		1994.5
(1.4)
P 		1662.5
(-1.9)
P 		2150.4
(3)
P 		1557.6
(-3)
P 		593
avr 1755.6
(-1) 		2059.9
(1.2) 		1876.4
(-0.1) 		2052.8
(1.2) 		2305.1
(3) 		1471.7
(-3) 		833
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185898_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9872
Sequence Type Exemplar sequence
Representative Public ID C28C12.7 NCBI
Target Description C28C12.7 /REP_DB=WormBase Gene ID /WP=CE04114 /TR=Q18276 /GB=AAB37550.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171400(11), NM_171399(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171400 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-10) (spp-10) mRNA, complete cds. 11/11 A 
NM_171399 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-10) (spp-10) mRNA, complete cds. 11/11 A 
SNAP00000012163 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8477165:8478110:1 11/11 None
GENEFINDER00000012181 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8477165:8478110:1 11/11 None
C28C12.7a.2 ENSEMBL cdna:known chromosome:CEL140:IV:8477162:8478146:1 gene:C28C12.7 11/11 None
C28C12.7a.1 ENSEMBL cdna:known chromosome:CEL140:IV:8477164:8478222:1 gene:C28C12.7 11/11 None
C28C12.7b ENSEMBL cdna:known chromosome:CEL140:IV:8477165:8480621:1 gene:C28C12.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8475389-8478116(+) 100.0 100.0
Public Domain and Genome References
Ensembl C28C12.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37549 Saposin-like protein family protein 10, isoform b [Caenorhabditis elegans] ref|NP_741465.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 0.0
blast AAB37550 Saposin-like protein family protein 10, isoform a [Caenorhabditis elegans] ref|NP_741466.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 1.0E-134
blast AAB37549 Saposin-like protein family protein 10, isoform b [Caenorhabditis elegans] ref|NP_741465.1| SaPosin-like Protein family member (spp-10) [Caenorhabditis elegans] 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR007856 EMBL-EBI Saposin-like type B, 1 1.8E-11
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 1.7E-9
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 4.7E-11
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 7.9E-11
Sequence
Target Sequence 
>C. ELEGANS:185898_AT
gaatgtcgtcttttcgtttcacaattggacaagttcattgacaaacttcagccattcttg
aagaatccacacgcagtctgcactcgtcttcatatctgctcaaacaagaaaatcgactca
ttccgccgcattttgctcgagttcgccaagcgcgctcaaaaaacttttgacgctccagcc
atcgtctgtgacgaatgccaatttgtcgtcaaggagttgaagactgttgttgaggacaag
aagagccaagctgaagcccgtgacttccttcgtgagaacgtgtgcaagagcctcggacag
tacagaggattctgcgatttggtcgttgacgagtatcttccacaattcatccaagagctt
gacgccattctccaagatccacaccaggtgtgcgttgacatcaaggcctgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGTCGTCTTTTCGTTTCACAAT 219 331 469 Antisense
CATTGACAAACTTCAGCCATTCTTG 667 215 504 Antisense
TTGAAGAATCCACACGCAGTCTGCA 630 703 526 Antisense
AACAAGAAAATCGACTCATTCCGCC 603 133 571 Antisense
GAGTTCGCCAAGCGCGCTCAAAAAA 341 397 607 Antisense
CAAAAAACTTTTGACGCTCCAGCCA 449 191 625 Antisense
GCTCCAGCCATCGTCTGTGACGAAT 54 299 640 Antisense
AGAAGAGCCAAGCTGAAGCCCGTGA 395 73 707 Antisense
CATCCAAGAGCTTGACGCCATTCTC 302 211 816 Antisense
TCTCCAAGATCCACACCAGGTGTGC 131 609 837 Antisense
TGTGCGTTGACATCAAGGCCTGCAA 622 555 857 Antisense
Sequence Source Affymetrix Proprietary Database