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[185896_at]

Description F57B10.8 /REP_DB=WormBase Gene ID /WP=CE11312 /TR=O44734 /GB=AAB96725.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 75      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 82      6grp_total > 76      4grp_total > 03      6grp_highest_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1745
1 2 3 4 range
A 400.2
(-2.2)
P 		451.2
(-2)
P 		228.2
(-3)
P 		1556.7
(3)
P 		1329
B 400.2
(-2.5)
P 		361.9
(-2.6)
P 		259.3
(-3)
P 		1888.9
(3)
P 		1630
C 405.4
(-2.6)
P 		296.2
(-3)
P 		286.9
(-3)
P 		1973.3
(3)
P 		1686
avr 401.9
(-2.4) 		369.8
(-2.6) 		258.1
(-3) 		1806.3
(3) 		1548

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 433
1 2 3 4 5 6 range
A 288.1
(-3)
P 		322.3
(-1.9)
P 		417.6
(1.3)
P 		318.6
(-2)
P 		333.2
(-1.5)
P 		469.2
(3)
P 		181
B 328.2
(-2.2)
P 		304.7
(-3)
P 		411.3
(0.7)
P 		360
(-1.1)
P 		361.3
(-1)
P 		477.3
(3)
P 		173
C 330
(-2.3)
P 		389.9
(-1.5)
P 		297.5
(-2.8)
P 		487.7
(-0.1)
P 		280.7
(-3)
P 		713.8
(3)
P 		433
avr 315.4
(-3) 		339
(-2.4) 		375.5
(-1.5) 		388.8
(-1.1) 		325.1
(-2.8) 		553.4
(3) 		238
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 343
1 2 3 4 range
A 145.7
(0.1)
P 		98
(-1.6)
P 		59.7
(-3)
P 		227.9
(3)
P 		168
B 127.6
(-1.8)
P 		89.4
(-2.5)
P 		60.1
(-3)
P 		402.5
(3)
P 		342
C 134.2
(-1.8)
P 		85.2
(-3)
P 		100
(-2.6)
P 		332.7
(3)
P 		248
avr 135.8
(-1.5) 		90.9
(-2.6) 		73.3
(-3) 		321
(3) 		248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 53.1
(-3)
P 		114.6
(3)
P 		90.9
(0.7)
P 		80.8
(-0.3)
P 		100.5
(1.6)
P 		114.2
(3)
P 		62
B 114.2
(-1.6)
P 		101.4
(-2.6)
P 		99.9
(-2.7)
P 		95.4
(-3)
P 		146.8
(0.7)
P 		178
(3)
P 		83
C 122.3
(-1.3)
P 		119
(-1.4)
P 		75.7
(-3)
P 		98.1
(-2.2)
P 		90.8
(-2.4)
P 		238.9
(3)
P 		163
avr 96.5
(-2.5) 		111.7
(-1.4) 		88.8
(-3) 		91.4
(-2.8) 		112.7
(-1.4) 		177
(3) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185896_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.371
Sequence Type Exemplar sequence
Representative Public ID F57B10.8 NCBI
Target Description F57B10.8 /REP_DB=WormBase Gene ID /WP=CE11312 /TR=O44734 /GB=AAB96725.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059486(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059486 NCBI Caenorhabditis elegans F57B10.8 (F57B10.8) mRNA, complete cds. 11/11 None
SNAP00000011826 ENSEMBL cdna:SNAP chromosome:CEL140:I:6556935:6558484:-1 11/11 None
GENEFINDER00000011841 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6556935:6558484:-1 11/11 None
F57B10.8 ENSEMBL cdna:known chromosome:CEL140:I:6556935:6558484:-1 gene:F57B10.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6556863-6558413(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F57B10.8
Entrez Gene 172368 Entrez gene
SwissProt O44734 EMBL-EBI
WormBase CE11312 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251690_AT TBP-binding protein, putative at
CANINE_2:CFA.17055.1.S1_AT similar to activator of basal transcription 1 cfa
CANINE_2:CFAAFFX.17148.1.S1_S_AT similar to activator of basal transcription 1 cfa
DROSOPHILA_2:1631251_AT dm
DROSGENOME1:143025_AT dm
HG-U133_PLUS_2:218405_AT activator of basal transcription 1 hs
HG-U133A:218405_AT activator of basal transcription 1 hs
HG-FOCUS:218405_AT activator of basal transcription 1 hs
HG-U133A_2:218405_AT activator of basal transcription 1 hs
HU35KSUBA:RC_AA598460_S_AT activator of basal transcription 1 hs
U133_X3P:G7019318_3P_AT activator of basal transcription 1 hs
HG-U95B:48108_AT activator of basal transcription 1 hs
HG-U95D:79634_AT activator of basal transcription 1 hs
HU35KSUBC:RC_W57798_AT Activator of basal transcription 1 hs
MG-U74AV2:160898_AT activator of basal transcription mm
MOE430A:1424751_AT activator of basal transcription mm
MOUSE430A_2:1424751_AT activator of basal transcription mm
MOUSE430_2:1424751_AT activator of basal transcription mm
MU11KSUBA:AA153127_S_AT TBP-binding protein ABT1 mm
MU19KSUBB:TC26665_AT TBP-binding protein ABT1 mm
MU19KSUBC:TC39578_AT TBP-binding protein ABT1 mm
RG-U34C:RC_AA818922_AT activator of basal transcription 1 (predicted) rn
RAE230A:1388601_AT activator of basal transcription 1 (predicted) rn
RAT230_2:1388601_AT activator of basal transcription 1 (predicted) rn
YEAST_2:1774175_AT Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator Sc
Protein Similarities
Method ID Description E-Value
blast AAB96725 Hypothetical protein F57B10.8 [Caenorhabditis elegans] ref|NP_491887.1| F57B10.8 [Caenorhabditis elegans] 1.0E-102
blast CAE67224 Hypothetical protein CBG12662 [Caenorhabditis briggsae] 2.0E-89
blast XP_796260 PREDICTED: similar to activator of basal transcription [Strongylocentrotus purpuratus] 8.0E-23
Sequence
Target Sequence 
>C. ELEGANS:185896_AT
aagactggtgttgtcttcttctcaacaattcctccaaaatacaacgtggttcgaatgcgt
gaatactttgaaaagcgatgccctggacaagttggtcgaatcttccttgcgcgtaacaag
cactcgaaaaatccgcaatatctatattcagaaggatggatggagttgaaaaagaaaaag
attgcaaaagcaattgctgaacaaattgataacactccgattggtggaaagaataaggat
ccagtgtcgagcatgttatggaatattagatatttatctggtttcaaatgggtacatctt
atggaacaattacaatatgaaaaagaagtggaaaaacgacgaatgaatgtggaagtcgca
caggctcgtcgaattgctgctcactttgaagaacaaattgagaaaggaaaacatttgaga
aagcttgaagcaaaggttacagcttccggtggaaagtgggataaattccagcgagatgtc
cagcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGACTGGTGTTGTCTTCTTCTCAA 594 153 250 Antisense
ACGTGGTTCGAATGCGTGAATACTT 232 93 293 Antisense
AAGCGATGCCCTGGACAAGTTGGTC 88 149 322 Antisense
CAAGTTGGTCGAATCTTCCTTGCGC 337 185 337 Antisense
TTCCTTGCGCGTAACAAGCACTCGA 186 701 352 Antisense
AGCACTCGAAAAATCCGCAATATCT 88 77 368 Antisense
AAGGATCCAGTGTCGAGCATGTTAT 281 161 484 Antisense
AAGTCGCACAGGCTCGTCGAATTGC 385 157 602 Antisense
CTCGTCGAATTGCTGCTCACTTTGA 437 231 614 Antisense
AGCAAAGGTTACAGCTTCCGGTGGA 521 71 678 Antisense
AAATTCCAGCGAGATGTCCAGCAGA 590 115 712 Antisense
Sequence Source Affymetrix Proprietary Database