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[185876_at]

Description K08B12.2 /REP_DB=WormBase Gene ID /WP=CE11912 /TR=O01582 /GB=AAB52258.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 38      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 50      6grp_total > 48      4grp_total > 02      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2159
1 2 3 4 range
A 480.2
(-2.6)
P 		374.3
(-2.9)
P 		356
(-3)
P 		2333.3
(3)
P 		1977
B 440
(-2.7)
P 		480.8
(-2.6)
P 		338.9
(-3)
P 		2497.7
(3)
P 		2159
C 607.5
(-2.2)
P 		529.3
(-2.5)
P 		403.4
(-3)
P 		2034.5
(3)
P 		1631
avr 509.2
(-2.6) 		461.5
(-2.7) 		366.1
(-3) 		2288.5
(3) 		1922

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 618
1 2 3 4 5 6 range
A 671.3
(2.7)
P 		380.2
(-1.9)
P 		316.1
(-2.9)
P 		309.7
(-3)
P 		315
(-2.9)
P 		691.8
(3)
P 		382
B 736.3
(3)
P 		374.5
(-1.9)
P 		378.8
(-1.8)
P 		344.4
(-2.3)
P 		288.9
(-3)
P 		558
(0.6)
P 		447
C 906.8
(3)
P 		375.5
(-2.4)
P 		314.3
(-3)
P 		409.3
(-2)
P 		413.6
(-2)
P 		538.6
(-0.7)
P 		593
avr 771.5
(3) 		376.7
(-2.4) 		336.4
(-3) 		354.5
(-2.8) 		339.2
(-3) 		596.1
(0.6) 		435
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 454
1 2 3 4 range
A 174.8
(-0.8)
P 		81.3
(-3)
P 		93.1
(-2.7)
P 		341.6
(3)
P 		260
B 140.3
(-2.2)
P 		118.8
(-2.5)
P 		78.5
(-3)
P 		532.3
(3)
P 		454
C 201.1
(-1.2)
P 		152.2
(-2.7)
P 		140.6
(-3)
P 		343
(3)
P 		202
avr 172.1
(-1.6) 		117.4
(-2.7) 		104.1
(-3) 		405.6
(3) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 123.8
(0.3)
P 		135.2
(1)
P 		68.8
(-3)
P 		78.5
(-2.4)
P 		95
(-1.4)
P 		168.4
(3)
P 		100
B 256.2
(3)
P 		124.7
(-1.8)
P 		92
(-3)
P 		91.2
(-3)
P 		117.4
(-2)
P 		208.1
(1.3)
P 		165
C 336.1
(3)
P 		114.6
(-2.2)
P 		80
(-3)
P 		82.3
(-2.9)
P 		133.8
(-1.7)
P 		180.3
(-0.7)
P 		256
avr 238.7
(3) 		124.8
(-1.3) 		80.3
(-3) 		84
(-2.9) 		115.4
(-1.7) 		185.6
(1) 		158
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185876_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11445
Sequence Type Exemplar sequence
Representative Public ID K08B12.2 NCBI
Target Description K08B12.2 /REP_DB=WormBase Gene ID /WP=CE11912 /TR=O01582 /GB=AAB52258.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171469(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171469 NCBI Caenorhabditis elegans K08B12.2a (K08B12.2) mRNA, complete cds. 11/11 None
SNAP00000019125 ENSEMBL cdna:SNAP chromosome:CEL140:V:6251880:6254323:1 11/11 None
GENEFINDER00000019132 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6249228:6254323:1 11/11 None
K08B12.2a ENSEMBL cdna:known chromosome:CEL140:V:6251766:6255047:1 gene:K08B12.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179012 2 1 NM_171469 174275_at (11), 185876_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6250489-6254323(+) 100.0 100.0
Public Domain and Genome References
Ensembl K08B12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52258 Hypothetical protein K08B12.2a [Caenorhabditis elegans] ref|NP_741551.1| K08B12.2a [Caenorhabditis elegans] 0.0
blast CAE71851 Hypothetical protein CBG18895 [Caenorhabditis briggsae] 1.0E-172
blast CAE71851 Hypothetical protein CBG18895 [Caenorhabditis briggsae] 1.0E-171
blast AAM45354 Hypothetical protein K08B12.2b [Caenorhabditis elegans] ref|NP_741552.1| K08B12.2b [Caenorhabditis elegans] 7.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR001275 EMBL-EBI DM DNA-binding 1.3E-27
Pfam IPR001275 EMBL-EBI DM DNA-binding 1.3E-27
Sequence
Target Sequence 
>C. ELEGANS:185876_AT
gggaattctctcagacttttcattgttgacaccagaaaccattacagccatcaagaacct
gttcaatcctcaaggaaatgatattgtgactacaacagctccaactccatcgatcttcga
caataccttcctgtcaaatcttaacattgcaaacggagtcacttacacgtcagccaatat
gaatggaaatcaaatgttgatgactcctcaagtcaccacagcatcgtcaaccggcatgtt
cccattcgaagatatcgacagaaagttgtcaaacggagattcaatgcttatcaatccagc
tgtttcacaactcaacgacctcaatattgcggtatcttctaataacacaatgtgcacaac
tgaattggctccaaaattgacgccacatatgtttgatactgcctgctctctcacaaccaa
tggagatcaatctccacccgagatcaaggtgaaagaaacgatgcatgagccaattggact
gcaaaacctgctgctctcaattgatgctccatctcgtaatcatccaaacttccaaatgtt
cctcgattgtgtgtgtagccta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGAATTCTCTCAGACTTTTCATTG 65 495 1005 Antisense
GACACCAGAAACCATTACAGCCATC 610 367 1032 Antisense
GATCTTCGACAATACCTTCCTGTCA 539 417 1116 Antisense
AAACGGAGTCACTTACACGTCAGCC 504 127 1155 Antisense
TGATGACTCCTCAAGTCACCACAGC 20 567 1202 Antisense
AATCCAGCTGTTTCACAACTCAACG 390 167 1297 Antisense
ACATATGTTTGATACTGCCTGCTCT 391 105 1389 Antisense
ATTGGACTGCAAAACCTGCTGCTCT 694 3 1477 Antisense
TGCTCTCAATTGATGCTCCATCTCG 352 581 1496 Antisense
ATCTCGTAATCATCCAAACTTCCAA 217 31 1515 Antisense
GTTCCTCGATTGTGTGTGTAGCCTA 599 441 1542 Antisense
Sequence Source Affymetrix Proprietary Database