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[185874_at]

Description F16G10.4 /REP_DB=WormBase Gene ID /WP=CE19393 /TR=O76588 /GB=AAC26272.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 33
(0.6)
A 		14.6
(-2.3)
A 		10.1
(-3)
A 		48
(3)
A 		38
B 31.7
(0.7)
A 		46.1
(3)
A 		8.5
(-3)
A 		27.5
(0)
A 		38
C 21.9
(-3)
A 		38.5
(-1.8)
A 		36.3
(-1.9)
A 		101.6
(3)
A 		80
avr 28.9
(-1.4) 		33.1
(-0.8) 		18.3
(-3) 		59
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 21.3
(0.7)
A 		8.9
(-2.3)
A 		30.9
(3)
A 		5.9
(-3)
A 		29.8
(2.7)
A 		9
(-2.3)
A 		25
B 13.3
(-0.9)
A 		29.1
(2.6)
A 		9.4
(-1.7)
A 		30.9
(3)
A 		6.5
(-2.3)
A 		3.5
(-3)
A 		27
C 18.6
(-0.8)
A 		44
(3)
A 		4.7
(-2.8)
A 		4.9
(-2.8)
A 		7.6
(-2.4)
A 		3.5
(-3)
A 		41
avr 17.7
(0.4) 		27.3
(3) 		15
(-0.4) 		13.9
(-0.7) 		14.6
(-0.5) 		5.3
(-3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 12
(3)
A 		3.2
(-2.6)
A 		2.6
(-3)
A 		7
(-0.2)
A 		9
B 10.1
(2.2)
A 		11.4
(3)
A 		2
(-3)
A 		5.9
(-0.5)
A 		9
C 7.2
(-3)
A 		11.1
(-0.6)
A 		12.6
(0.3)
A 		17.1
(3)
A 		10
avr 9.8
(2.7) 		8.6
(1) 		5.7
(-3) 		10
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 3.9
(-1.1)
A 		3.2
(-1.6)
A 		6.7
(1.2)
A 		1.5
(-3)
A 		9
(3)
A 		2.2
(-2.4)
A 		8
B 4.6
(-0.6)
A 		9.7
(3)
A 		2.3
(-2.3)
A 		8.2
(1.9)
A 		2.7
(-2)
A 		1.3
(-3)
A 		8
C 6.9
(-0.1)
A 		13.4
(3)
A 		1.2
(-2.9)
A 		1
(-3)
A 		2.5
(-2.3)
A 		1.2
(-2.9)
A 		12
avr 5.1
(-0.1) 		8.8
(3) 		3.4
(-1.5) 		3.6
(-1.3) 		4.7
(-0.4) 		1.6
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185874_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4788
Sequence Type Exemplar sequence
Representative Public ID F16G10.4 NCBI
Target Description F16G10.4 /REP_DB=WormBase Gene ID /WP=CE19393 /TR=O76588 /GB=AAC26272.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061856(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061856 NCBI Caenorhabditis elegans F16G10.4 (F16G10.4) mRNA, complete cds. 11/11 None
SNAP00000023114 ENSEMBL cdna:SNAP chromosome:CEL140:II:2369353:2370138:1 10/11 None
GENEFINDER00000023135 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2369332:2370605:1 10/11 None
F16G10.4 ENSEMBL cdna:known chromosome:CEL140:II:2369257:2370090:1 gene:F16G10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2369255-2370089(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F16G10.4
Entrez Gene 184579 Entrez gene
SwissProt O76588 EMBL-EBI
WormBase CE19393 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC26272 Hypothetical protein F16G10.4 [Caenorhabditis elegans] ref|NP_494257.1| F16G10.4 [Caenorhabditis elegans] 1.0E-115
blast AAC26272 Hypothetical protein F16G10.4 [Caenorhabditis elegans] ref|NP_494257.1| F16G10.4 [Caenorhabditis elegans] 2.0E-99
blast AAC26272 Hypothetical protein F16G10.4 [Caenorhabditis elegans] ref|NP_494257.1| F16G10.4 [Caenorhabditis elegans] 5.0E-95
blast AAF16621 Hypothetical protein F43C11.11 [Caenorhabditis elegans] ref|NP_494254.1| F43C11.11 [Caenorhabditis elegans] 2.0E-36
blast AAF16621 Hypothetical protein F43C11.11 [Caenorhabditis elegans] ref|NP_494254.1| F43C11.11 [Caenorhabditis elegans] 6.0E-35
blast AAF16621 Hypothetical protein F43C11.11 [Caenorhabditis elegans] ref|NP_494254.1| F43C11.11 [Caenorhabditis elegans] 1.0E-32
blast NP_508647 T14G12.6 [Caenorhabditis elegans] gb|AAA82438.1| Hypothetical protein T14G12.6 [Caenorhabditis elegans] 1.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 9.6E-84
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 5.4E-83
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 5.4E-83
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494257.1 1 7-29
F16G10.4 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:185874_AT
gtatacgacactttctgtctctttttattttaaaatgaatattttttggtcatttatcgt
gattgcacttgtgatgggcttcactgagagttgtatgcgaacggttccgccggatgatgc
ctatattcctgtcacgtcggctcctggagaaactgagatgcctggggaaatgctgacgac
aattgcaatggaagtcacaacagaaaaagtctgtgaagccacctcaactgcgacatgtcc
agatttggcgaatttttttatctcgccaacggagatcatccagcaagatggatgccctta
tttgaagtgtcagggaagcaaattgccaactcctttgtcggcgtacgatgttaccgaaat
tccaccacctgatgcatcgtacgaaacaactgcgtttaacatcaatcctccaacgacaga
tgcagagctaggtggcagttttatcaattattttggaattatttgtgatggtactgactg
gaagatcacgaagtacccaaatggaattgcatatcaggtcgctggcaatttcgttactgt
cggtgacgatgggtcctttgacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATACGACACTTTCTGTCTCTTTT 257 453 15 Antisense
GTCACGTCGGCTCCTGGAGAAACTG 297 465 145 Antisense
GATGCCTGGGGAAATGCTGACGACA 276 415 171 Antisense
AAAGTCTGTGAAGCCACCTCAACTG 56 123 220 Antisense
CACCTCAACTGCGACATGTCCAGAT 705 201 234 Antisense
GAAGCAAATTGCCAACTCCTTTGTC 318 341 329 Antisense
AACTCCTTTGTCGGCGTACGATGTT 62 143 342 Antisense
CACCACCTGATGCATCGTACGAAAC 697 201 377 Antisense
TTAACATCAATCCTCCAACGACAGA 181 685 410 Antisense
GGTCGCTGGCAATTTCGTTACTGTC 326 503 531 Antisense
GTCGGTGACGATGGGTCCTTTGACG 220 473 553 Antisense
Sequence Source Affymetrix Proprietary Database