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[185871_s_at]

Description C33C12.9 /REP_DB=WormBase Gene ID /WP=CE27815 /TR=O16582 /GB=AAB66061.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 04      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 519
1 2 3 4 range
A 141
(-2.3)
A 		87.2
(-3)
A 		104.1
(-2.8)
A 		532.5
(3)
P 		445
B 209.9
(-0.4)
A 		71.6
(-3)
A 		178.6
(-1)
A 		396.7
(3)
P 		325
C 169.7
(-1.9)
A 		102.2
(-2.7)
A 		77.4
(-3)
A 		590.6
(3)
P 		513
avr 173.5
(-1.8) 		87
(-3) 		120
(-2.5) 		506.6
(3) 		420

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 24.7
(-3)
A 		130.8
(0.5)
A 		138.2
(0.8)
A 		143
(0.9)
A 		138.2
(0.8)
A 		205.2
(3)
A 		181
B 130
(-1.1)
A 		163.6
(0.5)
A 		92
(-3)
A 		151.9
(-0.1)
A 		174.6
(1.1)
P 		214.3
(3)
P 		122
C 134.9
(1)
A 		92
(-0.4)
A 		18
(-3)
A 		79.6
(-0.9)
A 		164.6
(2.1)
P 		192
(3)
P 		174
avr 96.5
(-2.3) 		128.8
(-0.7) 		82.7
(-3) 		124.8
(-0.9) 		159.1
(0.8) 		203.8
(3) 		121
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 51.3
(0.3)
A 		18.9
(-3)
A 		27.2
(-2.2)
A 		78
(3)
P 		59
B 66.9
(1.4)
A 		17.7
(-3)
A 		41.4
(-0.9)
A 		84.5
(3)
P 		67
C 56.2
(-0.6)
A 		29.4
(-2.8)
A 		27
(-3)
A 		99.6
(3)
P 		73
avr 58.1
(0.3) 		22
(-3) 		31.9
(-2.1) 		87.4
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 4.6
(-3)
A 		46.5
(2.5)
A 		30.1
(0.4)
A 		36.3
(1.2)
A 		41.7
(1.9)
A 		50
(3)
A 		45
B 45.2
(-0.6)
A 		54.5
(0.4)
A 		22.3
(-3)
A 		40.2
(-1.1)
A 		70.9
(2.1)
P 		79.9
(3)
P 		58
C 50
(1.6)
A 		28.1
(-0.6)
A 		4.6
(-3)
A 		16
(-1.9)
A 		53.3
(1.9)
P 		64.3
(3)
P 		60
avr 33.3
(-1.1) 		43
(0.2) 		19
(-3) 		30.8
(-1.5) 		55.3
(1.8) 		64.7
(3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185871_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4481
Sequence Type Exemplar sequence
Representative Public ID C33C12.9 NCBI
Target Description C33C12.9 /REP_DB=WormBase Gene ID /WP=CE27815 /TR=O16582 /GB=AAB66061.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061808(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061808 NCBI Caenorhabditis elegans C33C12.9 (C33C12.9) mRNA, complete cds. 11/11 A 
SNAP00000027205 ENSEMBL cdna:SNAP chromosome:CEL140:II:2170897:2194822:-1 9/11 None
GENEFINDER00000027209 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2187217:2189458:-1 11/11 None
C33C12.9 ENSEMBL cdna:known chromosome:CEL140:II:2187217:2189752:-1 gene:C33C12.9 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2187215-2189751(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C33C12.9
Entrez Gene 173575 Entrez gene
SwissProt O16582 EMBL-EBI
WormBase CE27815 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256959_AT expressed protein at
CANINE_2:CFAAFFX.13598.1.S1_AT similar to N6-DNA-methyltransferase isoform 1 cfa
CHICKEN:GGA.1485.1.S1_AT N6-DNA-methyltransferase gga
HG-U133_PLUS_2:220311_AT chromosome 21 open reading frame 127 hs
HG-U133A:220311_AT chromosome 21 open reading frame 127 hs
HG-FOCUS:220311_AT chromosome 21 open reading frame 127 hs
HG-U133A_2:220311_AT chromosome 21 open reading frame 127 hs
U133_X3P:G7019462_3P_AT chromosome 21 open reading frame 127 hs
HG-U95D:82238_AT chromosome 21 open reading frame 127 hs
MOE430B:1453821_AT RIKEN cDNA 5830445C04 gene mm
MOUSE430_2:1453821_AT RIKEN cDNA 5830445C04 gene mm
MG-U74BV2:105417_S_AT RIKEN cDNA 5830445C04 gene mm
MG-U74BV2:107028_S_AT RIKEN cDNA 5830445C04 gene mm
MG-U74BV2:105418_R_AT RIKEN cDNA 5830445C04 gene mm
MOE430B:1430380_AT RIKEN cDNA 5830445C04 gene mm
MOUSE430_2:1430380_AT RIKEN cDNA 5830445C04 gene mm
MU19KSUBC:TC35944_AT RIKEN cDNA 5830445C04 gene, mRNA (cDNA clone MGC:106447 IMAGE:30548526) mm
RAE230B:1392330_AT similar to N6-DNA-methyltransferase isoform 1 (predicted) rn
RAT230_2:1392330_AT similar to N6-DNA-methyltransferase isoform 1 (predicted) rn
RG-U34B:RC_AI059282_AT Similar to N6-DNA-methyltransferase isoform 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6306 DNA methylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
8168 methyltransferase activity inferred from electronic annotation QuickGO AmiGO
8170 N-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494208 C33C12.8 [Caenorhabditis elegans] gb|AAB66060.1| Hypothetical protein C33C12.8 [Caenorhabditis elegans] 0.0
blast CAE70464 Hypothetical protein CBG17050 [Caenorhabditis briggsae] 0.0
blast CAE70469 Hypothetical protein CBG17056 [Caenorhabditis briggsae] 0.0
blast NP_494209 C33C12.9 [Caenorhabditis elegans] gb|AAB66061.2| Hypothetical protein C33C12.9 [Caenorhabditis elegans] 1.0E-129
blast NP_494209 C33C12.9 [Caenorhabditis elegans] gb|AAB66061.2| Hypothetical protein C33C12.9 [Caenorhabditis elegans] 1.0E-121
blast CAE73873 Hypothetical protein CBG21464 [Caenorhabditis briggsae] 1.0E-109
blast AAH97870 Unknown (protein for MGC:115637) [Xenopus laevis] 2.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:185871_S_AT
tactgacgaagagcccaaatcaaacatcgaattgacctacgctggcggccgaaccggtag
atcaacgctcgatcgtcttttgccacgtgtccccgagcttctgtccccacgtggtgtctt
ctatctcgtcgcacttcactcgaacgatatcccggcactgctcaaagagcactcggagca
aatgacggtgtcagtgtcaatggagcgccgttgcggcatcgaacatttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTGACGAAGAGCCCAAATCAAAC 582 645 507 Antisense
GCGGCCGAACCGGTAGATCAACGCT 688 287 551 Antisense
AGATCAACGCTCGATCGTCTTTTGC 468 65 565 Antisense
TCGTCGCACTTCACTCGAACGATAT 448 605 632 Antisense
CACTTCACTCGAACGATATCCCGGC 337 197 638 Antisense
TATCCCGGCACTGCTCAAAGAGCAC 448 657 654 Antisense
GGCACTGCTCAAAGAGCACTCGGAG 383 535 660 Antisense
GCACTCGGAGCAAATGACGGTGTCA 271 311 675 Antisense
ACGGTGTCAGTGTCAATGGAGCGCC 693 95 691 Antisense
CAATGGAGCGCCGTTGCGGCATCGA 408 181 704 Antisense
CGCCGTTGCGGCATCGAACATTTGT 229 257 712 Antisense
Sequence Source Affymetrix Proprietary Database