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[185862_at]

Description Y59A8B.20 /REP_DB=WormBase Gene ID /WP=CE24496 /TR=Q9NER7 /GB=CAB60948.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 35      6grp_total > 71      4grp_total > 57      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5545
1 2 3 4 range
A 1822.4
(-2.1)
P 		6080
(2.8)
P 		6263.8
(3)
P 		1003.9
(-3)
P 		5260
B 1875.7
(-2.1)
P 		5485.7
(1.8)
P 		6548.6
(3)
P 		1078.8
(-3)
P 		5470
C 1819.1
(-2.1)
P 		5807.7
(3)
P 		5654.3
(2.8)
P 		1085.6
(-3)
P 		4722
avr 1839.1
(-2.1) 		5791.1
(2.6) 		6155.6
(3) 		1056.1
(-3) 		5100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4864
1 2 3 4 5 6 range
A 2573.5
(-1)
P 		3976.4
(0.9)
P 		3876.2
(0.8)
P 		5567.1
(3)
P 		3565.8
(0.3)
P 		1052.7
(-3)
P 		4514
B 2379.2
(-1)
P 		4074.5
(1.6)
P 		3872.7
(1.3)
P 		4988.4
(3)
P 		3777
(1.1)
P 		1094.8
(-3)
P 		3894
C 2109.5
(-1.9)
P 		4106.1
(0.7)
P 		3773.5
(0.3)
P 		5917.1
(3)
P 		3536
(-0)
P 		1211.8
(-3)
P 		4705
avr 2354.1
(-1.3) 		4052.3
(1) 		3840.8
(0.7) 		5490.9
(3) 		3626.3
(0.4) 		1119.8
(-3) 		4371
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1824
1 2 3 4 range
A 663.5
(-0.9)
P 		1319.8
(1.7)
P 		1637.5
(3)
P 		147
(-3)
P 		1491
B 598.2
(-1.3)
P 		1354.8
(2.2)
P 		1517.5
(3)
P 		229.9
(-3)
P 		1288
C 602.2
(-1.6)
P 		1669.7
(2)
P 		1970.7
(3)
P 		183
(-3)
P 		1788
avr 621.3
(-1.3) 		1448.1
(2) 		1708.6
(3) 		186.6
(-3) 		1522

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1278
1 2 3 4 5 6 range
A 474.4
(-1.9)
P 		1414.2
(3)
P 		844.2
(0)
P 		1412
(3)
P 		1076
(1.2)
P 		256.3
(-3)
P 		1158
B 827.9
(-0.8)
P 		1356.2
(2.1)
P 		940.4
(-0.2)
P 		1321.3
(1.9)
P 		1534.5
(3)
P 		408.2
(-3)
P 		1126
C 781.9
(-0.3)
P 		1252.9
(3)
P 		960.4
(0.9)
P 		1189.9
(2.6)
P 		1144.2
(2.2)
P 		405.7
(-3)
P 		847
avr 694.7
(-0.9) 		1341.1
(3) 		915
(0.4) 		1307.7
(2.8) 		1251.6
(2.5) 		356.7
(-3) 		984
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185862_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14227
Sequence Type Exemplar sequence
Representative Public ID Y59A8B.20 NCBI
Target Description Y59A8B.20 /REP_DB=WormBase Gene ID /WP=CE24496 /TR=Q9NER7 /GB=CAB60948.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075119(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075119 NCBI Caenorhabditis elegans Y59A8B.20 (Y59A8B.20) mRNA, complete cds. 11/11 None
SNAP00000028694 ENSEMBL cdna:SNAP chromosome:CEL140:V:18001246:18003934:-1 10/11 None
GENEFINDER00000028712 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18001358:18003934:-1 11/11 None
Y59A8B.20 ENSEMBL cdna:known chromosome:CEL140:V:18001387:18003934:-1 gene:Y59A8B.20 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18001357-18003934(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y59A8B.20
Entrez Gene 190397 Entrez gene
SwissProt Q9NER7 EMBL-EBI
WormBase CE36755 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60948 Hypothetical protein Y59A8B.20 [Caenorhabditis elegans] ref|NP_507520.2| Y59A8B.20 [Caenorhabditis elegans] 1.0E-89
blast CAB60948 Hypothetical protein Y59A8B.20 [Caenorhabditis elegans] ref|NP_507520.2| Y59A8B.20 [Caenorhabditis elegans] 2.0E-84
blast CAB60948 Hypothetical protein Y59A8B.20 [Caenorhabditis elegans] ref|NP_507520.2| Y59A8B.20 [Caenorhabditis elegans] 4.0E-84
blast CAE61657 Hypothetical protein CBG05593 [Caenorhabditis briggsae] 9.0E-49
blast CAE61657 Hypothetical protein CBG05593 [Caenorhabditis briggsae] 3.0E-48
blast CAE61657 Hypothetical protein CBG05593 [Caenorhabditis briggsae] 8.0E-48
blast CAE61656 Hypothetical protein CBG05592 [Caenorhabditis briggsae] 4.0E-14
Sequence
Target Sequence 
>C. ELEGANS:185862_AT
aggaattcgcggttttgtgccattctggcagtgatctccgccatttcggtgtcatatgtt
cttgcacagagatcccaacagtcagtaattgcggatttgggaaagttgattgtggataac
tgcccacctatgttgtgcactggattggattgtgcagtcgttacagagaggaacgggtgc
cagctctgcgcatgtccaatcggatctccatcccgtggatgtgacccaatgccatttatc
ctttggcatgatcttattgtcaatggatgtccaaatgttacactcaacagtcgggaccct
gcacagaaagttcacagatggttcagacgagtgaatcgattcacgaacactgatcaatgt
gaaccctacatcttcccatactgtccagagttggatttcaatttgtggagatctccaaga
actaaacagccgatttcgatttacaggagcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGAATTCGCGGTTTTGTGCCATTC 302 55 16 Antisense
ATCTCCGCCATTTCGGTGTCATATG 647 33 49 Antisense
TGCACAGAGATCCCAACAGTCAGTA 541 577 78 Antisense
TGCCCACCTATGTTGTGCACTGGAT 278 585 136 Antisense
AGAGAGGAACGGGTGCCAGCTCTGC 347 69 180 Antisense
GGATCTCCATCCCGTGGATGTGACC 76 513 217 Antisense
GATGTGACCCAATGCCATTTATCCT 291 409 233 Antisense
TCAACAGTCGGGACCCTGCACAGAA 293 629 299 Antisense
ATCAATGTGAACCCTACATCTTCCC 353 27 368 Antisense
CATCTTCCCATACTGTCCAGAGTTG 440 213 384 Antisense
CAGCCGATTTCGATTTACAGGAGCC 676 201 442 Antisense
Sequence Source Affymetrix Proprietary Database