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[185860_s_at]

Description F22E5.12 /REP_DB=WormBase Gene ID /WP=CE09564 /TR=O16709 /GB=AAB66176.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 20      6grp_total > 57      4grp_total > 20      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 32.5
(-0.9)
P 		46
(0.1)
P 		5.4
(-3)
A 		84.4
(3)
P 		79
B 35.2
(-0.2)
A 		22.2
(-3)
A 		37.1
(0.2)
P 		50
(3)
A 		28
C 54.9
(2.3)
P 		36.5
(-0.8)
A 		23.4
(-3)
P 		59.3
(3)
A 		36
avr 40.9
(-0.3) 		34.9
(-1.2) 		22
(-3) 		64.6
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 4
(-3)
A 		29.8
(0.2)
A 		34.9
(0.9)
A 		52.1
(3)
A 		29.6
(0.2)
A 		49.9
(2.7)
A 		48
B 44.5
(3)
A 		18
(-2.3)
A 		14.4
(-3)
A 		31
(0.3)
M 		23
(-1.3)
A 		25.4
(-0.8)
P 		30
C 37.2
(1.7)
M 		32.6
(1)
A 		20.4
(-1)
A 		7.6
(-3)
A 		16.3
(-1.6)
A 		45.2
(3)
A 		38
avr 28.6
(-1) 		26.8
(-1.7) 		23.2
(-2.9) 		30.2
(-0.5) 		23
(-3) 		40.2
(3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 11.8
(2.7)
P 		10
(1.7)
P 		1.4
(-3)
A 		12.4
(3)
P 		11
B 11.2
(3)
A 		5.5
(-3)
A 		8.6
(0.3)
P 		10.6
(2.4)
A 		6
C 18.2
(3)
P 		10.5
(-1.6)
A 		8.2
(-3)
P 		10
(-1.9)
A 		10
avr 13.7
(3) 		8.7
(-0.9) 		6.1
(-3) 		11
(0.9) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 0.7
(-3)
A 		10.6
(1.8)
A 		7.6
(0.3)
A 		13.2
(3)
A 		8.9
(0.9)
A 		12.2
(2.5)
A 		13
B 15.5
(3)
A 		6
(-1.8)
A 		3.5
(-3)
A 		8.2
(-0.7)
M 		9.3
(-0.1)
A 		9.5
(0)
P 		12
C 13.8
(2.4)
M 		10
(0.8)
A 		5.2
(-1.4)
A 		1.5
(-3)
A 		5.3
(-1.3)
A 		15.1
(3)
A 		14
avr 10
(1) 		8.9
(0) 		5.4
(-3) 		7.6
(-1.1) 		7.8
(-0.9) 		12.3
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185860_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4880
Sequence Type Exemplar sequence
Representative Public ID F22E5.12 NCBI
Target Description F22E5.12 /REP_DB=WormBase Gene ID /WP=CE09564 /TR=O16709 /GB=AAB66176.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061917(11), NM_062076(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061917 NCBI Caenorhabditis elegans F22E5.12 (F22E5.12) mRNA, complete cds. 11/11 None
NM_062076 NCBI Caenorhabditis elegans ZC239.3 (ZC239.3) mRNA, complete cds. 11/11 None
SNAP00000023607 ENSEMBL cdna:SNAP chromosome:CEL140:II:2645431:2646844:-1 11/11 None
GENEFINDER00000023629 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2643686:2646844:-1 11/11 None
SNAP00000024295 ENSEMBL cdna:SNAP chromosome:CEL140:II:3216076:3216595:1 11/11 None
GENEFINDER00000024313 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3216076:3219272:1 11/11 None
F22E5.12 ENSEMBL cdna:known chromosome:CEL140:II:2646329:2646844:-1 gene:F22E5.12 11/11 None
ZC239.3 ENSEMBL cdna:known chromosome:CEL140:II:3216076:3216595:1 gene:ZC239.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3216074-3216594(+) 97.62 97.62
chrII:2646327-2646843(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl F22E5.12
ZC239.3
Entrez Gene 184840 Entrez gene
191120 Entrez gene
SwissProt O16709 EMBL-EBI
P91559 EMBL-EBI
WormBase CE09564 Wormbase
CE15116 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494317 F22E5.11 [Caenorhabditis elegans] gb|AAB66175.2| Hypothetical protein F22E5.11 [Caenorhabditis elegans] 1.0E-142
blast NP_494317 F22E5.11 [Caenorhabditis elegans] gb|AAB66175.2| Hypothetical protein F22E5.11 [Caenorhabditis elegans] 5.0E-99
blast AAB37943 Hypothetical protein ZC239.2 [Caenorhabditis elegans] ref|NP_494478.1| ZC239.2 [Caenorhabditis elegans] 6.0E-96
blast AAB37943 Hypothetical protein ZC239.2 [Caenorhabditis elegans] ref|NP_494478.1| ZC239.2 [Caenorhabditis elegans] 1.0E-66
blast NP_494318 F22E5.12 [Caenorhabditis elegans] gb|AAB66176.1| Hypothetical protein F22E5.12 [Caenorhabditis elegans] 1.0E-64
blast NP_494318 F22E5.12 [Caenorhabditis elegans] gb|AAB66176.1| Hypothetical protein F22E5.12 [Caenorhabditis elegans] 2.0E-64
blast AAB37942 Hypothetical protein ZC239.3 [Caenorhabditis elegans] ref|NP_494477.1| ZC239.3 [Caenorhabditis elegans] 3.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 7.2E-39
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 3.4E-38
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 1.1E-36
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 1.6E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000024313 1 183-205
Sequence
Target Sequence 
>C. ELEGANS:185860_S_AT
gtgatcaggctaaatattggtggcaaaaggtttgacacccataagtctactctcatgaaa
tttgatggatactttaagaaatttctgcagactcctggatctagtttggttgatccgatt
ttcgtcgaccgctctccgacatattttgatatcgttttgaactttatgagaaaaggccgt
gcagatctcccagaaactctgaaagacctgaagatgttgctgggtgaagccgaatattat
gagctgacagaattatgtgatgattgtaaacgagaaatttcaagattggatataggaata
gaatccgaaccgttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGATCAGGCTAAATATTGGTGGCA 682 483 34 Antisense
GTGGCAAAAGGTTTGACACCCATAA 342 491 53 Antisense
TGACACCCATAAGTCTACTCTCATG 626 571 66 Antisense
TAAGTCTACTCTCATGAAATTTGAT 100 637 75 Antisense
GAAATTTCTGCAGACTCCTGGATCT 594 363 111 Antisense
GACTCCTGGATCTAGTTTGGTTGAT 476 373 123 Antisense
TGGTTGATCCGATTTTCGTCGACCG 547 551 140 Antisense
ACCGCTCTCCGACATATTTTGATAT 398 89 161 Antisense
GAGAAAAGGCCGTGCAGATCTCCCA 221 391 201 Antisense
GAAGCCGAATATTATGAGCTGACAG 217 345 259 Antisense
TATAGGAATAGAATCCGAACCGTTT 133 655 324 Antisense
Sequence Source Affymetrix Proprietary Database