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[185855_s_at]

Description Y113G7A.4 /REP_DB=WormBase Gene ID /WP=CE23275 /TR=Q21609 /GB=CAB60477.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 13      4grp_highest_8_8 > 40      4grp_high_8_8 > 42      6grp_highest_nonorm > 32      6grp_total > 13      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 880
1 2 3 4 range
A 1207.5
(3)
P 		942.4
(0.4)
P 		814
(-0.9)
P 		596.2
(-3)
P 		611
B 1298.7
(3)
P 		1048.6
(1.3)
P 		817.8
(-0.3)
P 		418.4
(-3)
P 		880
C 1081.7
(2.4)
P 		1147.4
(3)
P 		820.5
(0.1)
P 		465.4
(-3)
P 		682
avr 1196
(3) 		1046.1
(1.7) 		817.4
(-0.2) 		493.3
(-3) 		703

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 724
1 2 3 4 5 6 range
A 1420.6
(1.6)
P 		1546.5
(3)
P 		1086
(-2.1)
P 		1029.1
(-2.8)
P 		1123.8
(-1.7)
P 		1007.4
(-3)
P 		539
B 1254.1
(1.6)
P 		1350.6
(3)
P 		1116.6
(-0.5)
P 		1280.1
(2)
P 		1007.6
(-2.1)
P 		946.4
(-3)
P 		404
C 1287.5
(1.5)
P 		1186
(0.5)
P 		1446
(3)
P 		1039
(-0.9)
P 		1117.3
(-0.2)
P 		823
(-3)
P 		623
avr 1320.7
(2.4) 		1361
(3) 		1216.2
(1) 		1116.1
(-0.4) 		1082.9
(-0.8) 		925.6
(-3) 		435
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 361
1 2 3 4 range
A 439.6
(3)
P 		204.6
(-1)
P 		212.8
(-0.9)
P 		87.3
(-3)
P 		352
B 414.1
(3)
P 		259
(0.1)
P 		189.5
(-1.1)
P 		89.2
(-3)
P 		325
C 358.1
(3)
P 		329.9
(2.4)
P 		286
(1.5)
P 		78.5
(-3)
P 		280
avr 403.9
(3) 		264.5
(0.4) 		229.4
(-0.3) 		85
(-3) 		319

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 341
1 2 3 4 5 6 range
A 261.9
(-2.5)
P 		550
(3)
P 		236.5
(-3)
P 		261
(-2.5)
P 		339.1
(-1)
P 		245.3
(-2.8)
P 		314
B 436.4
(2.6)
P 		449.6
(3)
P 		271.1
(-3)
P 		339.1
(-0.7)
P 		409.4
(1.6)
P 		352.9
(-0.3)
P 		179
C 477.2
(3)
P 		361.9
(0.4)
P 		368
(0.6)
P 		208.9
(-3)
P 		361.5
(0.4)
P 		275.5
(-1.5)
P 		268
avr 391.8
(1) 		453.8
(3) 		291.9
(-2.3) 		269.7
(-3) 		370
(0.3) 		291.2
(-2.3) 		184
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185855_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13862
Sequence Type Exemplar sequence
Representative Public ID Y113G7A.4 NCBI
Target Description Y113G7A.4 /REP_DB=WormBase Gene ID /WP=CE23275 /TR=Q21609 /GB=CAB60477.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075478(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075478 NCBI Caenorhabditis elegans Na/Ca eXchangers family member (ncx-1) (ncx-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000016737 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20103716:20118653:-1 10/11 None
Y113G7A.4 ENSEMBL cdna:known chromosome:CEL140:V:20103629:20118670:-1 gene:Y113G7A.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20103790-20118653(-) 99.85 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ncx-1
Ensembl Y113G7A.4
Entrez Gene 180318 Entrez gene
SwissProt Q21609 EMBL-EBI
WormBase CE23275 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:12369_AT magnesium/proton exchanger (MHX1) at
ATH1-121501:245127_AT magnesium/proton exchanger (MHX1) at
CANINE_2:CFAAFFX.7130.1.S1_S_AT similar to Sodium/calcium exchanger 2 precursor (Na(+)/Ca(2+)-exchange protein 2) cfa
HG-U133_PLUS_2:215267_S_AT solute carrier family 8 (sodium-calcium exchanger), member 2 hs
HG-U133A_2:215267_S_AT solute carrier family 8 (sodium-calcium exchanger), member 2 hs
HG-U133A:215267_S_AT solute carrier family 8 (sodium-calcium exchanger), member 2 hs
HU35KSUBA:RC_AA028942_AT solute carrier family 8 (sodium-calcium exchanger), member 2 hs
U133_X3P:HS.143026.0.S1_3P_S_AT solute carrier family 8 (sodium-calcium exchanger), member 2 hs
HG-U95AV2:35592_AT solute carrier family 8 (sodium-calcium exchanger), member 2 hs
HG-U95E:84722_AT solute carrier family 8 (sodium-calcium exchanger), member 2 hs
MOUSE430A_2:1428054_AT solute carrier family 8 (sodium/calcium exchanger), member 2 mm
MOE430A:1428054_AT solute carrier family 8 (sodium/calcium exchanger), member 2 mm
MOUSE430_2:1428054_AT solute carrier family 8 (sodium/calcium exchanger), member 2 mm
MOE430B:1438262_AT solute carrier family 8 (sodium/calcium exchanger), member 2 mm
MOUSE430_2:1438262_AT solute carrier family 8 (sodium/calcium exchanger), member 2 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6816 calcium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5432 calcium:sodium antiporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60477 Hypothetical protein Y113G7A.4 [Caenorhabditis elegans] ref|NP_507879.1| Na/Ca eXchangers family member (ncx-1) [Caenorhabditis elegans] emb|CAA62913.1| sodium-calcium exchanger [Caenorhabditis elegans] 0.0
blast CAE67427 Hypothetical protein CBG12917 [Caenorhabditis briggsae] 0.0
blast XP_686228 PREDICTED: similar to cardiac sodium-calcium exchanger [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 8.7E-27
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 4.1E-25
Pfam IPR003644 EMBL-EBI Na-Ca exchanger/integrin-beta4 1.5E-40
Pfam IPR003644 EMBL-EBI Na-Ca exchanger/integrin-beta4 1.2E-42
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 1.4E-8
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 1.6E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507879.1 8 70-92,164-186,199-221,226-248,703-725,776-798,811-833,848-870
Y113G7A.4 8 70-92,164-186,199-221,226-248,703-725,776-798,811-833,848-870
GENEFINDER00000016737 9 70-92,164-186,199-221,226-248,703-725,801-823,836-858,873-892,899-921
Sequence
Target Sequence 
>C. ELEGANS:185855_S_AT
atggaagctactgtttgccctgattccaccgactgactattttaatggttggctatgttt
tgtggttgcaattgcgatgattggactattaaccgcatttattggtgatatcgctgcagc
ttttgggtgtacagttgggctaaaggactctgtgaccgccctcaccttggtcgcaatggg
aacttctcttccagacacatttgcgtcccgcaccgccgcagttggagatcaatgggctga
cggatcgattggtaatgtgactggaagtaatgctgttaatgtattcttgggtattggaat
tgcctggatgattgctgcctgtgtacatgcctaccggggtaccaagtttttagtcgccac
aggctccttggccttctcggtaacaatgttcctaattggctcagtcgtatgtgtcgcctt
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGAAGCTACTGTTTGCCCTGATT 215 53 2082 Antisense
CTGATTCCACCGACTGACTATTTTA 153 239 2101 Antisense
TATCGCTGCAGCTTTTGGGTGTACA 277 657 2190 Antisense
TTGGGCTAAAGGACTCTGTGACCGC 230 707 2216 Antisense
TTGGTCGCAATGGGAACTTCTCTTC 365 707 2248 Antisense
TTCTCTTCCAGACACATTTGCGTCC 521 691 2265 Antisense
CACCGCCGCAGTTGGAGATCAATGG 332 201 2292 Antisense
GCCTGGATGATTGCTGCCTGTGTAC 69 283 2383 Antisense
TGTGTACATGCCTACCGGGGTACCA 567 553 2401 Antisense
CCTTGGCCTTCTCGGTAACAATGTT 608 267 2447 Antisense
TGGCTCAGTCGTATGTGTCGCCTTA 381 545 2478 Antisense
Sequence Source Affymetrix Proprietary Database