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[185853_at]

Description F36F12.4 /REP_DB=WormBase Gene ID /WP=CE17784 /TR=O76397 /GB=AAC19203.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 62      4grp_total > 10      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 3.6
(-3)
A 		3.6
(-3)
A 		4.8
(-1.7)
A 		9.1
(3)
A 		6
B 3.7
(3)
A 		2.6
(-1.7)
A 		2.3
(-3)
A 		3.5
(2.1)
A 		1
C 2.3
(-2.6)
A 		3.9
(3)
A 		2.2
(-3)
A 		2.9
(-0.5)
A 		2
avr 3.2
(-2.7) 		3.4
(-2.1) 		3.1
(-3) 		5.2
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 3.4
(-1.3)
A 		4.2
(0.6)
A 		2.7
(-3)
A 		5.2
(3)
A 		4.7
(1.8)
A 		2.9
(-2.5)
A 		3
B 2.2
(-2.1)
A 		3.8
(0.3)
A 		5.6
(3)
A 		1.6
(-3)
A 		3.1
(-0.8)
A 		2
(-2.4)
A 		4
C 3
(-1.3)
A 		2.8
(-1.7)
A 		4.1
(0.5)
A 		5.6
(3)
A 		2
(-3)
A 		3.9
(0.2)
A 		4
avr 2.9
(-3) 		3.6
(0.5) 		4.1
(3) 		4.1
(3) 		3.3
(-1) 		2.9
(-3) 		1
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 1.3
(3)
A 		0.8
(-3)
A 		1.2
(1.8)
A 		1.3
(3)
A 		1
B 1.2
(3)
A 		0.6
(-2.1)
A 		0.5
(-3)
A 		0.8
(-0.4)
A 		1
C 0.8
(0)
A 		1.1
(3)
A 		0.8
(0)
A 		0.5
(-3)
A 		1
avr 1.1
(3) 		0.8
(-3) 		0.8
(-3) 		0.9
(-1) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1
1 2 3 4 5 6 range
A 0.6
(-3)
A 		1.5
(3)
A 		0.6
(-3)
A 		1.3
(1.7)
A 		1.4
(2.3)
A 		0.7
(-2.3)
A 		1
B 0.8
(-0.6)
A 		1.3
(2.4)
A 		1.4
(3)
A 		0.4
(-3)
A 		1.3
(2.4)
A 		0.7
(-1.2)
A 		1
C 1.1
(1)
A 		0.9
(-1)
A 		1.1
(1)
A 		1.1
(1)
A 		0.7
(-3)
A 		1.3
(3)
A 		1
avr 0.8
(-3) 		1.2
(3) 		1
(0) 		0.9
(-1.5) 		1.1
(1.5) 		0.9
(-1.5) 		0
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185853_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14869
Sequence Type Exemplar sequence
Representative Public ID F36F12.4 NCBI
Target Description F36F12.4 /REP_DB=WormBase Gene ID /WP=CE17784 /TR=O76397 /GB=AAC19203.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071164(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071164 NCBI Caenorhabditis elegans F36F12.4 (F36F12.4) mRNA, complete cds. 11/11 None
SNAP00000033954 ENSEMBL cdna:SNAP chromosome:CEL140:V:2087124:2089575:1 9/11 None
GENEFINDER00000033966 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2086183:2091443:1 9/11 None
F36F12.4 ENSEMBL cdna:known chromosome:CEL140:V:2088252:2089575:1 gene:F36F12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2088251-2089575(+) 71.21 100.0
Public Domain and Genome References
Gene Title
Ensembl F36F12.4
Entrez Gene 185363 Entrez gene
SwissProt O76397 EMBL-EBI
WormBase CE17784 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE66795 Hypothetical protein CBG12156 [Caenorhabditis briggsae] 1.0E-135
blast AAC19203 Hypothetical protein F36F12.4 [Caenorhabditis elegans] ref|NP_503565.1| F36F12.4 [Caenorhabditis elegans] 1.0E-124
blast AAC19207 Hypothetical protein F36F12.8 [Caenorhabditis elegans] ref|NP_503566.1| F36F12.8 [Caenorhabditis elegans] 1.0E-117
blast CAE66795 Hypothetical protein CBG12156 [Caenorhabditis briggsae] 1.0E-115
blast CAE73921 Hypothetical protein CBG21533 [Caenorhabditis briggsae] 1.0E-69
blast AAC19203 Hypothetical protein F36F12.4 [Caenorhabditis elegans] ref|NP_503565.1| F36F12.4 [Caenorhabditis elegans] 3.0E-68
blast CAE73921 Hypothetical protein CBG21533 [Caenorhabditis briggsae] 2.0E-55
blast CAE66795 Hypothetical protein CBG12156 [Caenorhabditis briggsae] 2.0E-51
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 4.2E-6
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.6E-34
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 5.2E-38
Sequence
Target Sequence 
>C. ELEGANS:185853_AT
tctgcggcgcgcaatttttcgaataaaatttgactatcttgccacggggttctggccttc
ctcattgaatttttcgcgctccattgacaatcgcctgccggacaacgcgtgggaaagtgt
cgtgtactccacacggacaaatgccattagttttacaacaaaaatcgagccgcgacgcga
cacgcaacgcgccgtaaatctaccccagatatggccgagccaaaatggcctagcgcgacg
gattcaaaaaaccgaaaactcaccgcagaaattcaccaatctgcaagacaccatcgaaaa
cctggaggcgctgaaattccggaacactactagaactcaaatctgggaatcttcaaaaag
tatataccgcccggaaatggtcgccgtacaaacctaccatcacactcatctacagtatcc
cggcgttatcgtgaaaaatgttcccagggagactgccgggtttagacactaccgaatgac
ctccgtcagaaatagtatcggaaatggttggatttacggcgacaacttcactata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGCGGCGCGCAATTTTTCGAATA 471 609 60 Antisense
TGGCCTAGCGCGACGGATTCAAAAA 24 539 285 Antisense
AACTCACCGCAGAAATTCACCAATC 232 141 316 Antisense
TCACCAATCTGCAAGACACCATCGA 7 625 332 Antisense
GAGGCGCTGAAATTCCGGAACACTA 695 403 364 Antisense
AATGGTCGCCGTACAAACCTACCAT 9 161 435 Antisense
ACCATCACACTCATCTACAGTATCC 60 87 455 Antisense
TACAGTATCCCGGCGTTATCGTGAA 103 643 470 Antisense
ATGTTCCCAGGGAGACTGCCGGGTT 314 15 497 Antisense
TTAGACACTACCGAATGACCTCCGT 511 679 521 Antisense
GATTTACGGCGACAACTTCACTATA 423 415 570 Antisense
Sequence Source Affymetrix Proprietary Database