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[185845_at]

Description Y24F12A.1 /REP_DB=WormBase Gene ID /WP=CE28111 /TR=Q9U2Q7 /GB=CAB60325.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 97      4grp_highest_8_8 > 45      4grp_high_8_8 > 09      6grp_highest_nonorm > 93      6grp_total > 96      4grp_total > 15      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 352.3
(0.4)
P 		235.5
(-3)
P 		257.1
(-2.4)
P 		442.6
(3)
P 		207
B 335.7
(3)
P 		281.1
(-2)
P 		269.8
(-3)
P 		318.8
(1.5)
P 		66
C 380.7
(3)
P 		265.7
(-2.8)
P 		261.3
(-3)
P 		293.9
(-1.4)
P 		119
avr 356.2
(3) 		260.8
(-3) 		262.7
(-2.9) 		351.8
(2.7) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 386
1 2 3 4 5 6 range
A 357.2
(-2.4)
P 		330.6
(-3)
P 		384.3
(-1.7)
P 		426.6
(-0.8)
P 		513.7
(1.3)
P 		587.1
(3)
P 		257
B 350.2
(-3)
P 		368
(-2.6)
P 		373.9
(-2.5)
P 		449.7
(-1)
P 		586.5
(1.7)
P 		654.4
(3)
P 		304
C 268.7
(-3)
P 		335.8
(-1.5)
P 		327.5
(-1.7)
P 		531.6
(2.8)
P 		433.2
(0.6)
P 		540.3
(3)
P 		272
avr 325.4
(-3) 		344.8
(-2.6) 		361.9
(-2.2) 		469.3
(0.2) 		511.1
(1.1) 		593.9
(3) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 128.3
(3)
P 		51.1
(-3)
P 		67.2
(-1.7)
P 		64.8
(-1.9)
P 		77
B 107.1
(3)
P 		69.4
(-2.1)
P 		62.5
(-3)
P 		67.9
(-2.3)
P 		45
C 126
(3)
P 		76.4
(-0.9)
P 		91.1
(0.3)
P 		49.6
(-3)
P 		76
avr 120.5
(3) 		65.6
(-2.5) 		73.6
(-1.7) 		60.8
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 65.8
(-3)
P 		117.6
(0.5)
P 		83.7
(-1.8)
P 		108.2
(-0.1)
P 		155
(3)
P 		142.9
(2.2)
P 		89
B 121.9
(-1.8)
P 		122.5
(-1.8)
P 		90.8
(-3)
P 		119.1
(-1.9)
P 		238.3
(2.8)
P 		244
(3)
P 		153
C 99.6
(-2)
P 		102.5
(-1.8)
P 		83.3
(-3)
P 		106.9
(-1.5)
P 		140.2
(0.5)
P 		180.9
(3)
P 		98
avr 95.8
(-2.4) 		114.2
(-1.4) 		85.9
(-3) 		111.4
(-1.5) 		177.8
(2.3) 		189.3
(3) 		103
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185845_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8429
Sequence Type Exemplar sequence
Representative Public ID Y24F12A.1 NCBI
Target Description Y24F12A.1 /REP_DB=WormBase Gene ID /WP=CE28111 /TR=Q9U2Q7 /GB=CAB60325.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069787(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069787 NCBI Caenorhabditis elegans Y24F12A.1 (Y24F12A.1) mRNA, complete cds. 11/11 None
GENEFINDER00000037638 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11605278:11607309:1 11/11 None
Y24F12A.1 ENSEMBL cdna:known chromosome:CEL140:IV:11605278:11607427:1 gene:Y24F12A.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000037633 4/11 Cross Hyb Matching Probes None
SNAP00000037634 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11605283-11607315(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y24F12A.1
Entrez Gene 178085 Entrez gene
SwissProt Q9U2Q7 EMBL-EBI
WormBase CE28111 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60325 Hypothetical protein Y24F12A.1 [Caenorhabditis elegans] ref|NP_502188.1| Y24F12A.1 [Caenorhabditis elegans] 0.0
blast T26559 hypothetical protein Y24F12A.b - Caenorhabditis elegans 0.0
blast CAE62090 Hypothetical protein CBG06116 [Caenorhabditis briggsae] 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR001130 EMBL-EBI TatD-related deoxyribonuclease 2.3E-73
Pfam IPR001130 EMBL-EBI TatD-related deoxyribonuclease 2.3E-73
Sequence
Target Sequence 
>C. ELEGANS:185845_AT
gaatgggtgttttctggaccacaatggaatatattctatcagatgcagaagaatggaaag
tgttagctgtcggagaatgtggaatcgacttacatcgctgtgaatcagaattagaagtac
aaaaagatgtatttgagaagcatgttgcactagctttcaagttcaaactcccacttgtaa
ttcattgtcggaatggaagcaaaggccgggctgaggaagaatgtttgcagattttgaaaa
agaaaatggacgtaaatcataaatttctgaaaattcatcgacactgcttcacggagaatt
gggaagttgcacaaatgtggctgaaacaatgccctgatgtgcattttggattcacgccag
ctgtattcacaatgggagctccgcaaattcaagccatccgaaacattccactcgacagga
ttcttctcgaaacagatggaccctactttaaaccaaaatgctacgatggagttgctcctc
caaaagtttgtctaccgggaatggcgattgctacagcttcgaaaatagcggaaatcaaaa
atcttccaatagaagaagttctcagagccacattcaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGGGTGTTTTCTGGACCACAAT 140 333 707 Antisense
GTGGAATCGACTTACATCGCTGTGA 509 491 785 Antisense
GCATGTTGCACTAGCTTTCAAGTTC 34 311 846 Antisense
AACTCCCACTTGTAATTCATTGTCG 5 143 872 Antisense
AAATTCATCGACACTGCTTCACGGA 511 115 977 Antisense
TGGATTCACGCCAGCTGTATTCACA 39 551 1053 Antisense
GGAGCTCCGCAAATTCAAGCCATCC 276 521 1081 Antisense
ATTCCACTCGACAGGATTCTTCTCG 419 7 1111 Antisense
GAGTTGCTCCTCCAAAAGTTTGTCT 387 397 1175 Antisense
GGAATGGCGATTGCTACAGCTTCGA 80 533 1204 Antisense
GAAGTTCTCAGAGCCACATTCAACA 357 337 1261 Antisense
Sequence Source Affymetrix Proprietary Database