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[185835_at]

Description K08B12.1 /REP_DB=WormBase Gene ID /WP=CE11910 /TR=O01584 /GB=AAB52257.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 70      6grp_highest_nonorm > 53      6grp_total > 71      4grp_total > 49      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1915
1 2 3 4 range
A 6.7
(-3)
A 		1921.3
(3)
P 		1814.1
(2.7)
P 		22.8
(-2.9)
A 		1915
B 7.9
(-3)
A 		1740.8
(3)
P 		1592.9
(2.5)
P 		14.1
(-3)
A 		1733
C 22.6
(-3)
A 		1521.3
(2.7)
P 		1590.5
(3)
P 		108
(-2.7)
A 		1568
avr 12.4
(-3) 		1727.8
(3) 		1665.8
(2.8) 		48.3
(-2.9) 		1715

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1042
1 2 3 4 5 6 range
A 613.7
(-1.2)
P 		1072.7
(1.6)
P 		868.7
(0.3)
P 		1311.9
(3)
P 		870.2
(0.4)
P 		311
(-3)
P 		1001
B 600
(-1.1)
P 		1117.7
(2.1)
P 		898.6
(0.7)
P 		1263.6
(3)
P 		1005.8
(1.4)
P 		303.4
(-3)
P 		960
C 562.8
(-1.7)
P 		1071.1
(1.4)
P 		809.7
(-0.2)
P 		1345.7
(3)
P 		829.1
(-0.1)
P 		344
(-3)
P 		1002
avr 592.2
(-1.3) 		1087.2
(1.7) 		859
(0.3) 		1307.1
(3) 		901.7
(0.5) 		319.5
(-3) 		988
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 552
1 2 3 4 range
A 2.4
(-3)
A 		417.1
(2.3)
P 		474.2
(3)
P 		3.3
(-3)
A 		472
B 2.5
(-3)
A 		429.9
(3)
P 		369.1
(2.1)
P 		3
(-3)
A 		427
C 7.5
(-3)
A 		437.4
(1.7)
P 		554.4
(3)
P 		18.2
(-2.9)
A 		547
avr 4.1
(-3) 		428.1
(2.5) 		465.9
(3) 		8.2
(-2.9) 		462

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 333
1 2 3 4 5 6 range
A 113.1
(-2.3)
P 		381.5
(3)
P 		189.2
(-0.8)
P 		332.7
(2)
P 		262.6
(0.7)
P 		75.7
(-3)
P 		306
B 208.8
(-1.1)
P 		372
(2.3)
P 		218.2
(-0.9)
P 		334.7
(1.5)
P 		408.6
(3)
P 		113.1
(-3)
P 		296
C 208.6
(-0.4)
P 		326.8
(3)
P 		206.1
(-0.4)
P 		270.6
(1.4)
P 		268.3
(1.3)
P 		115.2
(-3)
P 		212
avr 176.8
(-1.2) 		360.1
(3) 		204.5
(-0.6) 		312.7
(1.9) 		313.2
(1.9) 		101.3
(-3) 		259
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185835_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11685
Sequence Type Exemplar sequence
Representative Public ID K08B12.1 NCBI
Target Description K08B12.1 /REP_DB=WormBase Gene ID /WP=CE11910 /TR=O01584 /GB=AAB52257.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072199(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072199 NCBI Caenorhabditis elegans K08B12.1 (K08B12.1) mRNA, complete cds. 10/11 None
SNAP00000004368 ENSEMBL cdna:SNAP chromosome:CEL140:V:6262484:6263315:1 11/11 None
GENEFINDER00000004372 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6262627:6263315:1 10/11 None
K08B12.1 ENSEMBL cdna:known chromosome:CEL140:V:6262041:6263315:1 gene:K08B12.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6262064-6263315(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08B12.1
Entrez Gene 179014 Entrez gene
SwissProt O01584 EMBL-EBI
WormBase CE35337 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4806 triacylglycerol lipase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52257 Hypothetical protein K08B12.1 [Caenorhabditis elegans] ref|NP_504600.2| K08B12.1 [Caenorhabditis elegans] 0.0
blast CAE71854 Hypothetical protein CBG18898 [Caenorhabditis briggsae] 0.0
blast AAB52257 Hypothetical protein K08B12.1 [Caenorhabditis elegans] ref|NP_504600.2| K08B12.1 [Caenorhabditis elegans] 1.0E-145
blast CAE71854 Hypothetical protein CBG18898 [Caenorhabditis briggsae] 1.0E-143
blast AAB52257 Hypothetical protein K08B12.1 [Caenorhabditis elegans] ref|NP_504600.2| K08B12.1 [Caenorhabditis elegans] 1.0E-118
blast CAE71854 Hypothetical protein CBG18898 [Caenorhabditis briggsae] 1.0E-115
blast CAB11554 Hypothetical protein Y49E10.18 [Caenorhabditis elegans] ref|NP_499623.2| Y49E10.18 [Caenorhabditis elegans] 5.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR002921 EMBL-EBI Lipase, class 3 1.5E-48
Pfam IPR002921 EMBL-EBI Lipase, class 3 6.1E-49
Pfam IPR002921 EMBL-EBI Lipase, class 3 2.9E-42
Sequence
Target Sequence 
>C. ELEGANS:185835_AT
gttttcgctgagatgcttcatggattgactgctcaacttggagcatgggagaaatttgag
tcgcaggatgcaggagtaatcaattacttccatactgcattctacagacttttcattgat
agtggaatggaagatgatctagttgatttgatgaagaaacacagaaactatagagtttgg
ttgactgggcactctctcggaggatctcttgcttccatgacagcgctccatcttgtcaag
aagaagtctgtcgataaaaatcgcatccgccttatcacgttcggtgagccacgtactgga
aacattgcttacgccaaggaaatcgaagagaacattccattccgttatcgagtaatcaag
cgtggagatccagttccaaatatgccagctccactcaatccagcagttcttactgcagct
caatataatagacaagcaatgcattaccgatatctggtgcattatgataacaatatggga
aagggagatggattcaatgtgtgctccgtagctgatgatcttggatgtcggaacaccgcc
atgagcaatttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTTCGCTGAGATGCTTCATGGAT 106 449 508 Antisense
AATTACTTCCATACTGCATTCTACA 105 177 589 Antisense
GAGTTTGGTTGACTGGGCACTCTCT 351 397 680 Antisense
TGACAGCGCTCCATCTTGTCAAGAA 555 571 725 Antisense
ATCACGTTCGGTGAGCCACGTACTG 276 25 781 Antisense
GAGAACATTCCATTCCGTTATCGAG 621 391 835 Antisense
GGAGATCCAGTTCCAAATATGCCAG 23 519 871 Antisense
TCCACTCAATCCAGCAGTTCTTACT 128 589 897 Antisense
GTGTGCTCCGTAGCTGATGATCTTG 537 487 1006 Antisense
TGATCTTGGATGTCGGAACACCGCC 184 565 1023 Antisense
TCGGAACACCGCCATGAGCAATTTG 416 605 1035 Antisense
Sequence Source Affymetrix Proprietary Database