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[185834_at]

Description ZK1127.3 /REP_DB=WormBase Gene ID /WP=CE28171 /TR=Q23403 /GB=AAB93425.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 77      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 82      6grp_total > 78      4grp_total > 14      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 224
1 2 3 4 range
A 287.7
(1.3)
P 		202.2
(-3)
P 		236.6
(-1.3)
P 		322.7
(3)
P 		121
B 314.8
(1.3)
P 		165.1
(-2.8)
P 		155.9
(-3)
P 		379.8
(3)
P 		224
C 300.6
(1)
P 		278.4
(-0)
P 		216.5
(-3)
P 		341.7
(3)
P 		125
avr 301
(1.1) 		215.2
(-2.5) 		203
(-3) 		348.1
(3) 		145

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 351
1 2 3 4 5 6 range
A 296.6
(-3)
P 		338
(-1.1)
P 		308.7
(-2.4)
P 		360.4
(-0.1)
P 		313.8
(-2.2)
P 		426.8
(3)
P 		130
B 256.4
(-2.4)
P 		283.5
(-1.7)
P 		272.4
(-2)
P 		234.1
(-3)
P 		385
(1)
P 		461.8
(3)
P 		228
C 362.3
(-1)
P 		283.1
(-2.4)
P 		246.9
(-3)
P 		289.2
(-2.2)
P 		374.4
(-0.7)
P 		585.2
(3)
P 		338
avr 305.1
(-2.2) 		301.5
(-2.3) 		276
(-3) 		294.6
(-2.5) 		357.7
(-0.7) 		491.3
(3) 		215
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 104.7
(3)
P 		43.9
(-3)
P 		61.9
(-1.2)
P 		47.2
(-2.7)
P 		61
B 100.4
(3)
P 		40.8
(-2.6)
P 		36.1
(-3)
P 		80.9
(1.2)
P 		64
C 99.5
(3)
P 		80
(0.2)
P 		75.4
(-0.5)
P 		57.6
(-3)
P 		42
avr 101.5
(3) 		54.9
(-3) 		57.8
(-2.6) 		61.9
(-2.1) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 54.7
(-3)
P 		120.2
(3)
P 		67.2
(-1.9)
P 		91.4
(0.4)
P 		94.7
(0.7)
P 		103.9
(1.5)
P 		66
B 89.2
(-1.5)
P 		94.4
(-1.2)
P 		66.1
(-2.8)
P 		62
(-3)
P 		156.4
(2.1)
P 		172.2
(3)
P 		110
C 134.3
(0.3)
P 		86.4
(-1.8)
P 		62.8
(-2.8)
P 		58.2
(-3)
P 		121.1
(-0.3)
P 		195.9
(3)
P 		138
avr 92.7
(-1.2) 		100.3
(-0.7) 		65.4
(-3) 		70.5
(-2.7) 		124.1
(0.8) 		157.3
(3) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185834_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5766
Sequence Type Exemplar sequence
Representative Public ID ZK1127.3 NCBI
Target Description ZK1127.3 /REP_DB=WormBase Gene ID /WP=CE28171 /TR=Q23403 /GB=AAB93425.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063047(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063047 NCBI Caenorhabditis elegans ZK1127.3 (ZK1127.3) mRNA, complete cds. 11/11 A 
SNAP00000033593 ENSEMBL cdna:SNAP chromosome:CEL140:II:7055227:7055943:1 11/11 None
GENEFINDER00000033606 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7055227:7055943:1 11/11 None
ZK1127.3 ENSEMBL cdna:known chromosome:CEL140:II:7055222:7056035:1 gene:ZK1127.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7055222-7055939(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK1127.3
Entrez Gene 174154 Entrez gene
SwissProt Q23403 EMBL-EBI
WormBase CE28171 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB93425 Hypothetical protein ZK1127.3 [Caenorhabditis elegans] ref|NP_495448.1| ZK1127.3 [Caenorhabditis elegans] 1.0E-117
blast B88197 protein ZK1127.3 [imported] - Caenorhabditis elegans 1.0E-109
blast AAB93425 Hypothetical protein ZK1127.3 [Caenorhabditis elegans] ref|NP_495448.1| ZK1127.3 [Caenorhabditis elegans] 1.0E-95
blast B88197 protein ZK1127.3 [imported] - Caenorhabditis elegans 2.0E-95
blast CAE73816 Hypothetical protein CBG21369 [Caenorhabditis briggsae] 3.0E-34
Sequence
Target Sequence 
>C. ELEGANS:185834_AT
attcgtcgtgattttttcggaccagttcaagaggcggcttgcaagccttatcttctagat
gttgatcctcgaaaatcgtggtgtgagctgtcagaactccgtctgagcactttaatctgc
gagtttaaaccgatcggaatcaataaacattttcatctaattggaataattgaacgtatg
ggaaaagtgtttgacaatgaaaatgctcaagtcgaaatatttctaagcgaagagaacaga
gttgcattccgtaaccaacaaagagacctcttgatgggaggacgcccagaacgaacttca
tatccgcctaaatacttgtgtcgtccttcacttgaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCGTCGTGATTTTTTCGGACCAG 597 9 46 Antisense
TTCGGACCAGTTCAAGAGGCGGCTT 701 683 61 Antisense
GGCTTGCAAGCCTTATCTTCTAGAT 470 537 81 Antisense
AGATGTTGATCCTCGAAAATCGTGG 436 61 102 Antisense
TGGTGTGAGCTGTCAGAACTCCGTC 437 553 124 Antisense
GAACTCCGTCTGAGCACTTTAATCT 543 347 139 Antisense
GAACAGAGTTGCATTCCGTAACCAA 262 351 279 Antisense
GAGACCTCTTGATGGGAGGACGCCC 636 393 308 Antisense
GCCCAGAACGAACTTCATATCCGCC 495 287 329 Antisense
ATCCGCCTAAATACTTGTGTCGTCC 427 37 347 Antisense
ACTTGTGTCGTCCTTCACTTGAAAT 117 105 359 Antisense
Sequence Source Affymetrix Proprietary Database