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[185832_at]

Description F16G10.8 /REP_DB=WormBase Gene ID /WP=CE19397 /TR=O76593 /GB=AAC26276.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 09      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 2
(-3)
A 		5.3
(-2.6)
A 		1.9
(-3)
A 		55.8
(3)
A 		54
B 4.4
(-3)
A 		22.3
(3)
A 		5.7
(-2.6)
A 		19.1
(1.9)
A 		18
C 5.8
(-2.6)
A 		3.2
(-3)
A 		25.3
(0.6)
A 		39.8
(3)
A 		37
avr 4.1
(-3) 		10.3
(-1.9) 		11
(-1.8) 		38.2
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 4.1
(-0.8)
A 		1.1
(-3)
A 		3.9
(-0.9)
A 		6.6
(1.1)
A 		1.5
(-2.7)
A 		9.2
(3)
A 		8
B 1.7
(-2.8)
A 		1.8
(-2.7)
A 		11.2
(0.9)
A 		2.8
(-2.3)
A 		16.7
(3)
A 		1.1
(-3)
A 		16
C 1.1
(-3)
A 		24.4
(0.2)
A 		2.4
(-2.8)
A 		44.7
(3)
A 		14.4
(-1.2)
A 		13.5
(-1.3)
A 		44
avr 2.3
(-3) 		9.1
(-0.4) 		5.8
(-1.7) 		18
(3) 		10.9
(0.3) 		7.9
(-0.9) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 0.7
(-2.8)
A 		1.2
(-2.5)
A 		0.5
(-3)
A 		8.2
(3)
A 		8
B 1.4
(-2.9)
A 		5.5
(3)
A 		1.3
(-3)
A 		4.1
(1)
A 		4
C 1.9
(-2.2)
A 		0.9
(-3)
A 		8.8
(3)
A 		6.7
(1.4)
A 		8
avr 1.3
(-3) 		2.5
(-1.6) 		3.5
(-0.4) 		6.3
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 0.8
(-1.7)
A 		0.4
(-3)
A 		0.9
(-1.3)
A 		1.7
(1.3)
A 		0.4
(-3)
A 		2.2
(3)
A 		2
B 0.6
(-2.8)
A 		0.6
(-2.8)
A 		2.7
(-0.8)
A 		0.7
(-2.7)
A 		6.8
(3)
A 		0.4
(-3)
A 		6
C 0.4
(-3)
A 		7.5
(2)
A 		0.6
(-2.9)
A 		9
(3)
A 		4.7
(0)
A 		4.5
(-0.1)
A 		9
avr 0.6
(-3) 		2.8
(0.9) 		1.4
(-1.6) 		3.8
(2.6) 		4
(3) 		2.4
(0.2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185832_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5718
Sequence Type Exemplar sequence
Representative Public ID F16G10.8 NCBI
Target Description F16G10.8 /REP_DB=WormBase Gene ID /WP=CE19397 /TR=O76593 /GB=AAC26276.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061861(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061861 NCBI Caenorhabditis elegans F16G10.8 (F16G10.8) mRNA, complete cds. 11/11 None
SNAP00000023118 ENSEMBL cdna:SNAP chromosome:CEL140:II:2381950:2383369:-1 11/11 None
F16G10.8 ENSEMBL cdna:known chromosome:CEL140:II:2381950:2383435:-1 gene:F16G10.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000023134 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2381948-2383434(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F16G10.8
Entrez Gene 184583 Entrez gene
SwissProt O76593 EMBL-EBI
WormBase CE19397 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC26276 Hypothetical protein F16G10.8 [Caenorhabditis elegans] ref|NP_494262.1| F16G10.8 [Caenorhabditis elegans] 1.0E-100
blast AAC26276 Hypothetical protein F16G10.8 [Caenorhabditis elegans] ref|NP_494262.1| F16G10.8 [Caenorhabditis elegans] 9.0E-88
blast AAC26277 Hypothetical protein F16G10.9 [Caenorhabditis elegans] ref|NP_494263.1| F16G10.9 [Caenorhabditis elegans] 2.0E-66
blast NP_508647 T14G12.6 [Caenorhabditis elegans] gb|AAA82438.1| Hypothetical protein T14G12.6 [Caenorhabditis elegans] 1.0E-61
blast NP_508647 T14G12.6 [Caenorhabditis elegans] gb|AAA82438.1| Hypothetical protein T14G12.6 [Caenorhabditis elegans] 9.0E-59
blast AAC26277 Hypothetical protein F16G10.9 [Caenorhabditis elegans] ref|NP_494263.1| F16G10.9 [Caenorhabditis elegans] 2.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 2.2E-57
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 3.8E-55
Sequence
Target Sequence 
>C. ELEGANS:185832_AT
ggatgatgtctatataccgagtacattggctcctgtggaaagtacaatggctcctggtga
aagcacagaggctccgatggctccttctaccacaaatgccgccgaggaaacaagtgtaac
tgaggtaatcctacaattgacaaatctctcaaaattccagctggtttcagaacaactatg
caaaattagtgaagccacgtgcccagatttggcgtcagtgctttcagctaacgtgatcat
cacaactgacacggaaggctgcattgaactatcgtgtgcatatggatttgttccatttct
tggtgcttcatttgatgacagtgaaataccaccacctactccaggtaccccagttacacc
gggagaaattgttgagaagttaaatggatactttggacttgtgtgtgagaataacaagtt
aacggccacagagtatccaattggaattggcactttttccggttctggtacttacggagc
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGATGTCTATATACCGAGTACA 609 513 90 Antisense
GTACAATGGCTCCTGGTGAAAGCAC 31 461 131 Antisense
GAAAGCACAGAGGCTCCGATGGCTC 25 361 148 Antisense
ATTTGGCGTCAGTGCTTTCAGCTAA 246 19 296 Antisense
GCTTTCAGCTAACGTGATCATCACA 278 305 309 Antisense
CATCACAACTGACACGGAAGGCTGC 593 209 327 Antisense
CGGAAGGCTGCATTGAACTATCGTG 673 241 341 Antisense
GGATACTTTGGACTTGTGTGTGAGA 160 511 475 Antisense
ATAACAAGTTAACGGCCACAGAGTA 223 23 500 Antisense
TGGAATTGGCACTTTTTCCGGTTCT 417 545 531 Antisense
TTCCGGTTCTGGTACTTACGGAGCA 94 689 546 Antisense
Sequence Source Affymetrix Proprietary Database