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[185824_at]

Description F16G10.6 /REP_DB=WormBase Gene ID /WP=CE19395 /TR=O76591 /GB=AAC26274.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 28      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 24.3
(-3)
A 		50.8
(1.6)
A 		50.5
(1.6)
A 		58.8
(3)
A 		35
B 41.5
(3)
P 		22.2
(-0.3)
A 		26.5
(0.4)
A 		6.9
(-3)
A 		35
C 31.4
(2)
A 		32.4
(2.2)
A 		2.7
(-3)
A 		37.2
(3)
A 		35
avr 32.4
(1.1) 		35.1
(3) 		26.6
(-3) 		34.3
(2.4) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 22.3
(1.9)
A 		18.9
(1)
A 		26.9
(3)
A 		2.9
(-3)
A 		21.4
(1.6)
A 		26.4
(2.9)
A 		24
B 33.7
(2.2)
A 		36.9
(3)
A 		17.7
(-2.1)
A 		26.9
(0.3)
A 		14.3
(-3)
A 		18.8
(-1.8)
A 		23
C 38.5
(3)
A 		34
(2.2)
A 		34.3
(2.2)
A 		10.1
(-2.3)
A 		26.4
(0.7)
A 		6.4
(-3)
A 		32
avr 31.5
(3) 		29.9
(2.5) 		26.3
(1.3) 		13.3
(-3) 		20.7
(-0.6) 		17.2
(-1.7) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 8.8
(-2.7)
A 		11
(0.1)
A 		13.2
(3)
A 		8.6
(-3)
A 		5
B 13.2
(3)
P 		5.5
(-0.9)
A 		6.1
(-0.6)
A 		1.5
(-3)
A 		12
C 10.4
(3)
A 		9.3
(2.3)
A 		0.9
(-3)
A 		6.3
(0.4)
A 		10
avr 10.8
(3) 		8.6
(0.5) 		6.7
(-1.6) 		5.5
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 4.1
(0.4)
A 		6.7
(3)
A 		5.8
(2.1)
A 		0.7
(-3)
A 		6.4
(2.7)
A 		6.4
(2.7)
A 		6
B 11.7
(2.6)
A 		12.3
(3)
A 		4.3
(-3)
A 		7.1
(-0.9)
A 		5.8
(-1.9)
A 		7
(-1)
A 		8
C 14.3
(3)
A 		10.4
(1.1)
A 		8.7
(0.3)
A 		2
(-3)
A 		8.5
(0.2)
A 		2.2
(-2.9)
A 		12
avr 10
(3) 		9.8
(2.8) 		6.3
(-0.3) 		3.3
(-3) 		6.9
(0.2) 		5.2
(-1.3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185824_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5347
Sequence Type Exemplar sequence
Representative Public ID F16G10.6 NCBI
Target Description F16G10.6 /REP_DB=WormBase Gene ID /WP=CE19395 /TR=O76591 /GB=AAC26274.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061859
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061859 NCBI Caenorhabditis elegans F16G10.6 (F16G10.6) mRNA, complete cds. None
F16G10.6 ENSEMBL cdna:known chromosome:CEL140:II:2377200:2377852:-1 gene:F16G10.6 None
SNAP00000023116 ENSEMBL cdna:SNAP chromosome:CEL140:II:2377200:2377840:-1 None
GENEFINDER00000023142 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2376884:2377840:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2377198-2377879(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F16G10.6
Entrez Gene 184581 Entrez gene
SwissProt O76591 EMBL-EBI
WormBase CE35491 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC26274 Hypothetical protein F16G10.6 [Caenorhabditis elegans] ref|NP_494260.2| F16G10.6 [Caenorhabditis elegans] 2.0E-81
blast AAC26274 Hypothetical protein F16G10.6 [Caenorhabditis elegans] ref|NP_494260.2| F16G10.6 [Caenorhabditis elegans] 1.0E-76
blast AAC26274 Hypothetical protein F16G10.6 [Caenorhabditis elegans] ref|NP_494260.2| F16G10.6 [Caenorhabditis elegans] 5.0E-32
blast AAF16614 Hypothetical protein F43C11.12 [Caenorhabditis elegans] ref|NP_494255.2| F43C11.12 [Caenorhabditis elegans] 2.0E-28
blast AAF16614 Hypothetical protein F43C11.12 [Caenorhabditis elegans] ref|NP_494255.2| F43C11.12 [Caenorhabditis elegans] 6.0E-28
blast AAC26272 Hypothetical protein F16G10.4 [Caenorhabditis elegans] ref|NP_494257.1| F16G10.4 [Caenorhabditis elegans] 1.0E-25
blast AAC26272 Hypothetical protein F16G10.4 [Caenorhabditis elegans] ref|NP_494257.1| F16G10.4 [Caenorhabditis elegans] 1.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 2.9E-78
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 2.3E-38
Pfam IPR003326 EMBL-EBI Protein of unknown function DUF130 3.1E-78
Sequence
Target Sequence 
>C. ELEGANS:185824_AT
atgcatctctttgtgtttataactctttacctttttgtccatggaatgtccaatcgacac
gttctcccaagaaaccactgcaagtgttgtaagtttgatgattttttgagagttgtttgc
cgagaccctgaaccttcaacgtgcggggacttgctttcctactttcttacacctgcggag
tatgtcgtacagaacggttgccttgtattaacttgttctaataacactttgccatttata
aaggctcaatttcaatttagtgaaataacaccaccaaatggtttttcctatttaactgct
tttgaaatcagccctccaaactcactggaagaaatgggtggtgccagcctagctaagcac
tttggaatcatttgtgatgacgacgtgtggaaggccacaaaatatccaaatggaattaca
tataccctagctccaccgagtttcgcggatggttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCATCTCTTTGTGTTTATAACTC 270 43 13 Antisense
ATAACTCTTTACCTTTTTGTCCATG 613 23 31 Antisense
TTCTCCCAAGAAACCACTGCAAGTG 249 693 74 Antisense
TTTGAGAGTTGTTTGCCGAGACCCT 344 665 117 Antisense
ACTTTCTTACACCTGCGGAGTATGT 31 103 173 Antisense
GAACGGTTGCCTTGTATTAACTTGT 676 345 204 Antisense
AACTGCTTTTGAAATCAGCCCTCCA 689 141 306 Antisense
CAGCCCTCCAAACTCACTGGAAGAA 375 203 321 Antisense
GTGGTGCCAGCCTAGCTAAGCACTT 344 489 350 Antisense
GGAATTACATATACCCTAGCTCCAC 598 533 424 Antisense
CTCCACCGAGTTTCGCGGATGGTTC 86 235 443 Antisense
Sequence Source Affymetrix Proprietary Database