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[185815_at]

Description K10F12.4 /REP_DB=WormBase Gene ID /WP=CE12092 /TR=O17234 /GB=AAB71002.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 09      4grp_highest_8_8 > 03      4grp_high_8_8 > 45      6grp_highest_nonorm > 64      6grp_total > 92      4grp_total > 42      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 604
1 2 3 4 range
A 318.3
(-3)
P 		918.4
(3)
P 		753.6
(1.4)
P 		452
(-1.7)
P 		600
B 332.2
(-3)
P 		836.8
(3)
P 		631.2
(0.6)
P 		446.2
(-1.6)
P 		505
C 349
(-2.9)
P 		922
(3)
P 		630.6
(0)
P 		335.7
(-3)
P 		586
avr 333.2
(-3) 		892.4
(3) 		671.8
(0.6) 		411.3
(-2.2) 		559

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 665
1 2 3 4 5 6 range
A 892.3
(2.4)
P 		927.2
(3)
P 		618.5
(-2.2)
P 		924.4
(3)
P 		923.1
(2.9)
P 		569.3
(-3)
P 		358
B 775.1
(1.4)
P 		830.1
(2.1)
P 		650
(-0.3)
P 		748.7
(1)
P 		899.7
(3)
P 		442.2
(-3)
P 		458
C 884.3
(0.4)
P 		682.4
(-2)
P 		960.5
(1.3)
P 		842.6
(-0.1)
P 		1107.4
(3)
P 		601.4
(-3)
P 		506
avr 850.6
(1.3) 		813.2
(0.8) 		743
(-0.2) 		838.6
(1.1) 		976.7
(3) 		537.6
(-3) 		439
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 209
1 2 3 4 range
A 115.9
(-0.8)
P 		199.4
(3)
P 		197
(2.9)
P 		66.2
(-3)
P 		133
B 106
(-2.4)
P 		206.7
(3)
P 		146.3
(-0.2)
P 		95.1
(-3)
P 		112
C 115.5
(-1.3)
P 		265.1
(3)
P 		219.8
(1.7)
P 		56.6
(-3)
P 		209
avr 112.5
(-1.4) 		223.7
(3) 		187.7
(1.6) 		72.6
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 164.5
(-2.1)
P 		329.8
(3)
P 		134.7
(-3)
P 		234.4
(0.1)
P 		278.5
(1.4)
P 		138.6
(-2.9)
P 		195
B 269.7
(0.2)
P 		276.3
(0.4)
P 		157.8
(-3)
P 		198.3
(-1.8)
P 		365.5
(3)
P 		164.9
(-2.8)
P 		208
C 327.8
(2)
P 		208.2
(-1.8)
P 		244.5
(-0.6)
P 		169.4
(-3)
P 		358.3
(3)
P 		201.3
(-2)
P 		189
avr 254
(0.1) 		271.4
(0.7) 		179
(-2.6) 		200.7
(-1.8) 		334.1
(3) 		168.3
(-3) 		166
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185815_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8253
Sequence Type Exemplar sequence
Representative Public ID K10F12.4 NCBI
Target Description K10F12.4 /REP_DB=WormBase Gene ID /WP=CE12092 /TR=O17234 /GB=AAB71002.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171062(9), NM_171061(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171062 NCBI Caenorhabditis elegans K10F12.4b (K10F12.4) mRNA, complete cds. 9/11 None
NM_171061 NCBI Caenorhabditis elegans K10F12.4a (K10F12.4) mRNA, complete cds. 11/11 None
SNAP00000031878 ENSEMBL cdna:SNAP chromosome:CEL140:III:376564:382047:-1 11/11 None
GENEFINDER00000031883 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:378011:381297:-1 11/11 None
K10F12.4b.1 ENSEMBL cdna:known chromosome:CEL140:III:378003:381297:-1 gene:K10F12.4 9/11 None
K10F12.4a ENSEMBL cdna:known chromosome:CEL140:III:378136:381297:-1 gene:K10F12.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
K10F12.4b.2 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:378135-381297(-) 100.0 100.0
Public Domain and Genome References
Ensembl K10F12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71002 Hypothetical protein K10F12.4a [Caenorhabditis elegans] ref|NP_741069.1| K10F12.4a [Caenorhabditis elegans] 1.0E-161
blast CAE69580 Hypothetical protein CBG15797 [Caenorhabditis briggsae] 1.0E-158
blast AAB71002 Hypothetical protein K10F12.4a [Caenorhabditis elegans] ref|NP_741069.1| K10F12.4a [Caenorhabditis elegans] 1.0E-149
blast CAE69495 Hypothetical protein CBG15701 [Caenorhabditis briggsae] 1.0E-139
blast CAE69580 Hypothetical protein CBG15797 [Caenorhabditis briggsae] 1.0E-138
blast CAE69495 Hypothetical protein CBG15701 [Caenorhabditis briggsae] 1.0E-138
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 0.0022
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 2.7E-19
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 0.0022
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 2.7E-19
Sequence
Target Sequence 
>C. ELEGANS:185815_AT
gtatgcggttttgtccatatgcacagagagtattgatctatttggccaagaagaatattc
cagtagaagtagtaaatgtaaatccggatagaagtcccaattggtaccttcccaagtcac
caatcggaagagttccagcacttgaaattaatggaaaagttgtgtgggaatcgaatgtca
ttgttgaataccttgatgaactattcccaactaacactattttgccacgagatgcatatg
agaaagctcaccagaaaattctagtggaacgattgtcgccgataatgaacgcgttattcg
aattctacggtagttccaataatccacaggctcaacgacaaaacgacatgaatgttcaca
gtgcgctaaggaattcggagaatttgctcagagacactttttacggaggccgtcaaccag
gatacgccgactatttgatgtggccattcttggaacgcctccaactgctcacaatgtctc
caaactcacaattccgatatttcccaggacttcattacccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATGCGGTTTTGTCCATATGCACA 567 455 320 Antisense
CAATTGGTACCTTCCCAAGTCACCA 475 167 417 Antisense
GAACTATTCCCAACTAACACTATTT 361 343 517 Antisense
GGTAGTTCCAATAATCCACAGGCTC 155 505 628 Antisense
CAGAGACACTTTTTACGGAGGCCGT 542 205 708 Antisense
TACGGAGGCCGTCAACCAGGATACG 501 615 721 Antisense
ACCAGGATACGCCGACTATTTGATG 53 87 735 Antisense
GATGTGGCCATTCTTGGAACGCCTC 592 407 756 Antisense
TGCTCACAATGTCTCCAAACTCACA 685 579 785 Antisense
AACTCACAATTCCGATATTTCCCAG 154 141 802 Antisense
ATATTTCCCAGGACTTCATTACCCG 522 19 816 Antisense
Sequence Source Affymetrix Proprietary Database