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[185812_at]

Description C04E12.2 /REP_DB=WormBase Gene ID /WP=CE16815 /TR=O76679 /GB=AAC27088.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 62      4grp_total > 58      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 192
1 2 3 4 range
A 125.1
(0.3)
P 		171.1
(2)
P 		198.3
(3)
P 		33.5
(-3)
A 		165
B 116.5
(0.1)
P 		95.1
(-0.6)
A 		198.5
(3)
P 		26.2
(-3)
A 		172
C 115.9
(-1.6)
P 		191
(1.8)
P 		218.3
(3)
P 		83.4
(-3)
A 		135
avr 119.2
(-0.3) 		152.4
(1) 		205
(3) 		47.7
(-3) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 407
1 2 3 4 5 6 range
A 84.1
(-3)
A 		257.3
(0.3)
P 		402.3
(3)
P 		257.2
(0.3)
P 		143.3
(-1.9)
P 		107.7
(-2.6)
A 		318
B 77.8
(-3)
A 		299.2
(0.3)
P 		484.3
(3)
P 		277.5
(-0.1)
P 		152.2
(-1.9)
P 		103.6
(-2.6)
A 		407
C 84.3
(-3)
A 		305.5
(1.8)
P 		363.4
(3)
P 		338.5
(2.5)
P 		132
(-2)
P 		130.4
(-2)
P 		279
avr 82.1
(-3) 		287.3
(0.7) 		416.7
(3) 		291.1
(0.7) 		142.5
(-1.9) 		113.9
(-2.4) 		335
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 45.5
(2.2)
P 		37.1
(1.1)
P 		51.8
(3)
P 		4.9
(-3)
A 		47
B 37.2
(1.7)
P 		23.5
(-0.3)
A 		46
(3)
P 		5.6
(-3)
A 		40
C 38.4
(-0.6)
P 		54.9
(0.9)
P 		76.1
(3)
P 		14.1
(-3)
A 		62
avr 40.4
(0.9) 		38.5
(0.7) 		58
(3) 		8.2
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 15.5
(-3)
A 		91.5
(3)
P 		87.6
(2.7)
P 		65.2
(0.9)
P 		43.3
(-0.8)
P 		26.2
(-2.2)
A 		76
B 27.1
(-3)
A 		99.6
(1.8)
P 		117.6
(3)
P 		73.5
(0.1)
P 		61.8
(-0.7)
P 		38.6
(-2.2)
A 		91
C 31.2
(-3)
A 		93.2
(3)
P 		92.5
(2.9)
P 		68.1
(0.6)
P 		42.7
(-1.9)
P 		43.7
(-1.8)
P 		62
avr 24.6
(-3) 		94.8
(2.6) 		99.2
(3) 		68.9
(0.6) 		49.3
(-1) 		36.2
(-2.1) 		75
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185812_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15577
Sequence Type Exemplar sequence
Representative Public ID C04E12.2 NCBI
Target Description C04E12.2 /REP_DB=WormBase Gene ID /WP=CE16815 /TR=O76679 /GB=AAC27088.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071537(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071537 NCBI Caenorhabditis elegans C04E12.2 (C04E12.2) mRNA, complete cds. 11/11 None
SNAP00000029751 ENSEMBL cdna:SNAP chromosome:CEL140:V:3368989:3369664:1 11/11 None
GENEFINDER00000029765 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3368989:3369664:1 11/11 None
C04E12.2 ENSEMBL cdna:known chromosome:CEL140:V:3368907:3369703:1 gene:C04E12.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_071510 2/11 Cross Hyb Matching Probes None
F35F10.4 2/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3368988-3369664(+) 100.0 100.0
chrV:3293145-3294895(+) 96.2 96.2
Public Domain and Genome References
Gene Title
Ensembl C04E12.2
Entrez Gene 182208 Entrez gene
SwissProt O76679 EMBL-EBI
WormBase CE16815 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC27088 Hypothetical protein C04E12.2 [Caenorhabditis elegans] ref|NP_503938.1| C04E12.2 [Caenorhabditis elegans] 1.0E-103
blast T15149 hypothetical protein F35F10.3 - Caenorhabditis elegans 3.0E-75
blast AAC04398 Hypothetical protein C17B7.4 [Caenorhabditis elegans] ref|NP_503926.1| C17B7.4 [Caenorhabditis elegans] 1.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR002542 EMBL-EBI Protein of unknown function DUF19 2.3E-81
Sequence
Target Sequence 
>C. ELEGANS:185812_AT
tgcgaaacttactcaaatttgctctacttggaacactactccttacattcgcgtatgggg
cctacaaacctccaaacaagagcgattgcaagggagtcactgttggaaaggtgttcacct
gtctttaccgagccggagattacatggcaaaaacattctttctagacataaagaaggcaa
gcagtttgttggaattcagcaaatcatgcatagccatgcaaaactgtcttgaggctctga
ggtgtggacagggaactgaacatcttgcgatgtacgaaagaatgagagggcaatgtgtga
ccatgcacttctattctacagactttttctcttgtctgactaagttagaaaattcgaagc
cgaaacctgaatgctatgagaaatgggacactaaattagtcaacgaagatttaacagagg
tgagcccggacaaattgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCGAAACTTACTCAAATTTGCTCT 514 583 14 Antisense
ATTTGCTCTACTTGGAACACTACTC 619 17 30 Antisense
CTACTCCTTACATTCGCGTATGGGG 146 227 49 Antisense
ACATTCGCGTATGGGGCCTACAAAC 304 103 58 Antisense
GGCCTACAAACCTCCAAACAAGAGC 249 469 72 Antisense
GAGCGATTGCAAGGGAGTCACTGTT 259 387 93 Antisense
GAAAGGTGTTCACCTGTCTTTACCG 412 361 119 Antisense
ACCTGTCTTTACCGAGCCGGAGATT 375 87 130 Antisense
TGACCATGCACTTCTATTCTACAGA 382 569 311 Antisense
TATTCTACAGACTTTTTCTCTTGTC 25 665 325 Antisense
CAGAGGTGAGCCCGGACAAATTGTG 83 207 428 Antisense
Sequence Source Affymetrix Proprietary Database