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[185811_at]

Description Y71H2B.4 /REP_DB=WormBase Gene ID /WP=CE24631 /TR=Q9N4F0 /GB=AAF36033.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 95      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 48      6grp_total > 75      4grp_total > 02      6grp_high1_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18848
1 2 3 4 range
A 1808.6
(-3)
P 		2368
(-2.8)
P 		1882.8
(-3)
P 		20564.6
(3)
P 		18756
B 1716.8
(-3)
P 		1981.8
(-2.9)
P 		1976.8
(-2.9)
P 		17074.2
(3)
P 		15357
C 1864.9
(-3)
P 		2003.5
(-2.9)
P 		1842.1
(-3)
P 		18222.3
(3)
P 		16380
avr 1796.8
(-3) 		2117.8
(-2.9) 		1900.6
(-3) 		18620.4
(3) 		16824

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2656
1 2 3 4 5 6 range
A 3909.8
(-0.4)
P 		2756.6
(-3)
P 		3874.9
(-0.4)
P 		4469.7
(0.9)
P 		3830.4
(-0.5)
P 		5368.2
(3)
P 		2612
B 3295
(-1.8)
P 		2772
(-3)
P 		4385.7
(0.7)
P 		3418.1
(-1.5)
P 		4352.5
(0.6)
P 		5412.7
(3)
P 		2641
C 3029.9
(-2.5)
P 		2827.1
(-3)
P 		4112.7
(0)
P 		4604.8
(1.2)
P 		4013.9
(-0.2)
P 		5395
(3)
P 		2568
avr 3411.6
(-1.6) 		2785.2
(-3) 		4124.4
(0.1) 		4164.2
(0.2) 		4065.6
(-0.1) 		5392
(3) 		2607
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3180
1 2 3 4 range
A 658.5
(-2.6)
P 		514
(-2.9)
P 		492.2
(-3)
P 		3010.3
(3)
P 		2518
B 547.5
(-2.8)
P 		489.5
(-2.9)
P 		458.1
(-3)
P 		3638.5
(3)
P 		3180
C 617.3
(-2.9)
P 		576
(-3)
P 		642
(-2.8)
P 		3072.6
(3)
P 		2497
avr 607.8
(-2.8) 		526.5
(-3) 		530.8
(-3) 		3240.5
(3) 		2714

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1298
1 2 3 4 5 6 range
A 720.7
(-3)
P 		980.4
(-0.3)
P 		843.9
(-1.7)
P 		1133.6
(1.2)
P 		1155.9
(1.5)
P 		1307
(3)
P 		586
B 1146.5
(-1.7)
P 		922.7
(-2.9)
P 		1065
(-2.1)
P 		905.3
(-3)
P 		1768.4
(1.7)
P 		2018.4
(3)
P 		1113
C 1123.1
(-1.3)
P 		862.6
(-3)
P 		1046.7
(-1.8)
P 		926
(-2.6)
P 		1298.8
(-0.2)
P 		1806
(3)
P 		943
avr 996.8
(-2.4) 		921.9
(-3) 		985.2
(-2.5) 		988.3
(-2.5) 		1407.7
(0.7) 		1710.5
(3) 		789
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185811_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6729
Sequence Type Exemplar sequence
Representative Public ID Y71H2B.4 NCBI
Target Description Y71H2B.4 /REP_DB=WormBase Gene ID /WP=CE24631 /TR=Q9N4F0 /GB=AAF36033.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065189(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065189 NCBI Caenorhabditis elegans Y71H2B.4 (Y71H2B.4) mRNA, complete cds. 11/11 None
SNAP00000014010 ENSEMBL cdna:SNAP chromosome:CEL140:III:2618979:2622330:1 11/11 None
GENEFINDER00000014050 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2618979:2619313:1 11/11 None
Y71H2B.4 ENSEMBL cdna:known chromosome:CEL140:III:2618952:2619357:1 gene:Y71H2B.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2618978-2619313(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2B.4
Entrez Gene 175381 Entrez gene
SwissProt Q9N4F0 EMBL-EBI
WormBase CE24631 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF36032 Hypothetical protein Y71H2B.3 [Caenorhabditis elegans] ref|NP_497591.1| Y71H2B.3 [Caenorhabditis elegans] 1.0E-172
blast CAE64991 Hypothetical protein CBG09827 [Caenorhabditis briggsae] 1.0E-138
blast AAF36033 Hypothetical protein Y71H2B.4 [Caenorhabditis elegans] ref|NP_497590.1| Y71H2B.4 [Caenorhabditis elegans] 1.0E-49
blast AAF36033 Hypothetical protein Y71H2B.4 [Caenorhabditis elegans] ref|NP_497590.1| Y71H2B.4 [Caenorhabditis elegans] 2.0E-47
blast CAE64990 Hypothetical protein CBG09826 [Caenorhabditis briggsae] 5.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR007304 EMBL-EBI TAP42-like protein 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:185811_AT
ttcgcctcgtcatgcaagaatttcgataaatatatgaatatgttctgccgttacggcttg
gagcaatccccatgcactgttgaggattatagcacctacaaatccgcgtgctgtgccatg
aagaacaactgctcgtacaaggaattcccgaaggatgatgtctgctgcttcactgttgag
tgcctcaagagatgcttcccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGCCTCGTCATGCAAGAATTTCG 209 697 52 Antisense
ATGAATATGTTCTGCCGTTACGGCT 200 39 85 Antisense
TGCCGTTACGGCTTGGAGCAATCCC 531 585 97 Antisense
GCAATCCCCATGCACTGTTGAGGAT 341 321 114 Antisense
GCACCTACAAATCCGCGTGCTGTGC 297 315 143 Antisense
TCCGCGTGCTGTGCCATGAAGAACA 96 597 154 Antisense
GTGCCATGAAGAACAACTGCTCGTA 196 479 164 Antisense
CAACTGCTCGTACAAGGAATTCCCG 551 187 177 Antisense
TTCCCGAAGGATGATGTCTGCTGCT 624 695 196 Antisense
TGCTGCTTCACTGTTGAGTGCCTCA 232 583 214 Antisense
TGAGTGCCTCAAGAGATGCTTCCCG 24 569 228 Antisense
Sequence Source Affymetrix Proprietary Database