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[185810_s_at]

Description T21D12.3 /REP_DB=WormBase Gene ID /WP=CE18267 /TR=O16786 /GB=AAC48091.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 95      4grp_highest_8_8 > 22      4grp_high_8_8 > 14      6grp_highest_nonorm > 91      6grp_total > 85      4grp_total > 09      6grp_highest_sub > In      6grp_high1_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 448
1 2 3 4 range
A 133.4
(-3)
P 		174.2
(-2.5)
P 		219.9
(-1.8)
P 		581.1
(3)
P 		448
B 140.1
(-3)
P 		173.2
(-2.5)
P 		154.3
(-2.8)
P 		507.4
(3)
P 		367
C 154.4
(-3)
P 		159.9
(-2.9)
P 		161.3
(-2.9)
P 		563.8
(3)
P 		409
avr 142.6
(-3) 		169.1
(-2.6) 		178.5
(-2.5) 		550.8
(3) 		408

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 125.1
(-3)
P 		166.5
(-1)
P 		200.3
(0.6)
P 		134.4
(-2.6)
P 		249.8
(3)
P 		226.9
(1.9)
P 		125
B 123.8
(-1.6)
P 		99.2
(-3)
P 		175.1
(1.2)
P 		135
(-1)
P 		124.8
(-1.6)
P 		208.5
(3)
P 		109
C 173
(-2.5)
P 		244.6
(0.8)
P 		162.2
(-3)
P 		291.9
(3)
P 		230.5
(0.2)
P 		223.7
(-0.2)
P 		130
avr 140.6
(-3) 		170.1
(-0.8) 		179.2
(-0.1) 		187.1
(0.5) 		201.7
(1.6) 		219.7
(3) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 48.6
(-1.6)
P 		37.8
(-3)
P 		57.5
(-0.5)
P 		85.1
(3)
P 		47
B 44.7
(-2.3)
P 		42.8
(-2.4)
P 		35.8
(-3)
P 		108.1
(3)
P 		72
C 51.1
(-2.4)
P 		46
(-3)
P 		56.2
(-1.8)
P 		95.1
(3)
P 		49
avr 48.1
(-2.3) 		42.2
(-3) 		49.8
(-2.2) 		96.1
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 23.1
(-3)
P 		59.2
(1.1)
P 		43.6
(-0.6)
P 		34.1
(-1.7)
P 		75.4
(3)
P 		55.2
(0.7)
P 		52
B 43.1
(-1.6)
P 		33
(-3)
P 		42.5
(-1.7)
P 		35.8
(-2.6)
P 		50.7
(-0.6)
P 		77.7
(3)
P 		45
C 64.1
(1.1)
P 		74.6
(2.9)
P 		41.3
(-3)
P 		58.7
(0.1)
P 		74.6
(2.9)
P 		74.9
(3)
P 		34
avr 43.4
(-2.8) 		55.6
(-0.1) 		42.5
(-3) 		42.9
(-2.9) 		66.9
(2.5) 		69.3
(3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185810_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11186
Sequence Type Exemplar sequence
Representative Public ID T21D12.3 NCBI
Target Description T21D12.3 /REP_DB=WormBase Gene ID /WP=CE18267 /TR=O16786 /GB=AAC48091.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067489(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067489 NCBI Caenorhabditis elegans T21D12.3 (T21D12.3) mRNA, complete cds. 9/11 A 
SNAP00000019185 ENSEMBL cdna:SNAP chromosome:CEL140:IV:260200:264224:-1 9/11 None
GENEFINDER00000019203 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:260056:264224:-1 11/11 None
T21D12.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:259984:261685:-1 gene:T21D12.3 9/11 None
T21D12.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:259989:261683:-1 gene:T21D12.3 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:260199-261680(-) 99.44 100.0
Public Domain and Genome References
Gene Title
Ensembl T21D12.3
Entrez Gene 176847 Entrez gene
SwissProt O16786 EMBL-EBI
WormBase CE31622 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15090_AT WW domain-containing protein at
ATH1-121501:267068_AT WW domain-containing protein at
ATH1-121501:267067_AT WW domain-containing protein at
CANINE:1584979_AT similar to polyglutamine binding protein 1 isoform d cfa
CANINE_2:CFA.11739.1.A1_S_AT similar to polyglutamine binding protein 1 isoform d cfa
CANINE_2:CFAAFFX.24012.1.S1_S_AT similar to polyglutamine binding protein 1 isoform d cfa
HG-U133_PLUS_2:207769_S_AT polyglutamine binding protein 1 hs
HG-U133A:207769_S_AT polyglutamine binding protein 1 hs
HG-FOCUS:207769_S_AT polyglutamine binding protein 1 hs
HG-U133A_2:207769_S_AT polyglutamine binding protein 1 hs
HG-U133_PLUS_2:210499_S_AT polyglutamine binding protein 1 hs
HG-U133A:210499_S_AT polyglutamine binding protein 1 hs
HG-U133A_2:210499_S_AT polyglutamine binding protein 1 hs
HG-U133_PLUS_2:214527_S_AT polyglutamine binding protein 1 hs
HG-U133A_2:214527_S_AT polyglutamine binding protein 1 hs
HG-U133A:214527_S_AT polyglutamine binding protein 1 hs
HU35KSUBA:RC_AA179787_AT polyglutamine binding protein 1 hs
U133_X3P:G10801589_3P_X_AT polyglutamine binding protein 1 hs
U133_X3P:G10801589_3P_AT polyglutamine binding protein 1 hs
U133_X3P:G10801589_3P_A_AT polyglutamine binding protein 1 hs
U133_X3P:HS.30570.4.S1_3P_X_AT polyglutamine binding protein 1 hs
U133_X3P:HS.30570.4.S1_3P_A_AT polyglutamine binding protein 1 hs
U133_X3P:210499_3P_S_AT polyglutamine binding protein 1 hs
HG-U95AV2:34325_AT polyglutamine binding protein 1 hs
MU11KSUBA:AA231619_S_AT polyglutamine binding protein 1 mm
MG-U74AV2:93010_AT polyglutamine binding protein 1 mm
MOE430A:1456086_X_AT polyglutamine binding protein 1 mm
MOUSE430A_2:1456086_X_AT polyglutamine binding protein 1 mm
MOUSE430_2:1456086_X_AT polyglutamine binding protein 1 mm
MOE430A:1417731_AT polyglutamine binding protein 1 mm
MOUSE430A_2:1417731_AT polyglutamine binding protein 1 mm
MOUSE430_2:1417731_AT polyglutamine binding protein 1 mm
MG-U74CV2:140192_AT Polyglutamine binding protein 1, mRNA (cDNA clone MGC:6586 IMAGE:3484619) mm
RG-U34B:RC_AA964301_AT similar to Polyglutamine binding protein 1 rn
RG-U34B:RC_AA998881_F_AT similar to Polyglutamine binding protein 1 rn
RAE230A:1372797_AT similar to Polyglutamine binding protein 1 rn
RAT230_2:1372797_AT similar to Polyglutamine binding protein 1 rn
Protein Similarities
Method ID Description E-Value
blast AAC48090 Paralysed arrest at two-fold protein 6 [Caenorhabditis elegans] ref|NP_499891.1| Paralysed Arrest at Two-fold family member (pat-6) [Caenorhabditis elegans] sp|O16785|PARV_CAEEL Paralyzed arrest at two-fold protein 6 (Actopaxin homolog) (Parvin-like protein) 0.0
blast AAC48091 Hypothetical protein T21D12.3 [Caenorhabditis elegans] ref|NP_499890.2| T21D12.3 [Caenorhabditis elegans] 1.0E-169
blast AAC48091 Hypothetical protein T21D12.3 [Caenorhabditis elegans] ref|NP_499890.2| T21D12.3 [Caenorhabditis elegans] 1.0E-168
blast AAC48091 Hypothetical protein T21D12.3 [Caenorhabditis elegans] ref|NP_499890.2| T21D12.3 [Caenorhabditis elegans] 1.0E-165
blast CAE74731 Hypothetical protein CBG22549 [Caenorhabditis briggsae] 1.0E-164
blast CAE65556 Hypothetical protein CBG10545 [Caenorhabditis briggsae] 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 2.5E-6
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 2.5E-6
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.5E-8
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 4.3E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000019203 1 680-702
Sequence
Target Sequence 
>C. ELEGANS:185810_S_AT
attgggttgaagtctatgatgagggacttgcgaagtattatttctggaataaaacaacag
atgaggtttgttggtactcgcctcgtcatccacgagctattatcagtgatccagctcctc
gaatcgccagagagcatgctacagtgctttttggagattctcacgcatctgaggtagaat
ttgaagctgaaatcgtacttaatgggtatcgccgccggaaaaaagaggacaatgccgccc
gccgcagaaatttccacaacaagaaaacttcacgcggagggaatgatgataagcaatcac
gtggcaataatgctgaaaagcggaagcgacgtgatccgatgacgtcacagggtccagatt
cagatgaagacgagttacaggaaatgacaaataaggatagactgaaaagagcaaaaagga
aaggaattgatccaatggatccagcagcttatggagatgctccagttggaaaatggtcgg
atggtcttcgagttgatcaggtgactggagccgacgtga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGGGTTGAAGTCTATGATGAGGG 193 5 293 Antisense
GGACTTGCGAAGTATTATTTCTGGA 14 525 316 Antisense
TGAGGTTTGTTGGTACTCGCCTCGT 648 563 354 Antisense
TCATCCACGAGCTATTATCAGTGAT 56 623 378 Antisense
AGCTATTATCAGTGATCCAGCTCCT 188 79 387 Antisense
TCACGCATCTGAGGTAGAATTTGAA 559 623 453 Antisense
ATCGTACTTAATGGGTATCGCCGCC 568 33 484 Antisense
ATCCAATGGATCCAGCAGCTTATGG 82 41 722 Antisense
TCCAGCAGCTTATGGAGATGCTCCA 607 589 732 Antisense
TGGTCGGATGGTCTTCGAGTTGATC 284 549 766 Antisense
GATCAGGTGACTGGAGCCGACGTGA 381 421 787 Antisense
Sequence Source Affymetrix Proprietary Database