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[185804_at]

Description Y32B12A.3 /REP_DB=WormBase Gene ID /WP=CE19060 /TR=Q9XXH0 /GB=CAA19479.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 28      6grp_total > 19      4grp_total > 41      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 46.6
(-2.4)
A 		44.1
(-2.8)
A 		82.2
(3)
M 		42.8
(-3)
A 		39
B 39
(-1.8)
M 		65.6
(3)
M 		32
(-3)
P 		57.5
(1.6)
P 		34
C 62.8
(-3)
P 		84.8
(3)
P 		78.6
(1.3)
A 		70.2
(-1)
P 		22
avr 49.5
(-3) 		64.8
(3) 		64.3
(2.8) 		56.8
(-0.1) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 48.5
(3)
P 		38.4
(1)
P 		44.4
(2.2)
P 		18.4
(-3)
A 		47.8
(2.9)
P 		45.6
(2.4)
M 		30
B 50.1
(1.8)
A 		21.1
(-1.9)
A 		33.8
(-0.3)
A 		59.4
(3)
A 		46.8
(1.4)
A 		12.6
(-3)
A 		47
C 61.1
(3)
A 		47.8
(1.1)
A 		36.7
(-0.4)
A 		37.1
(-0.3)
A 		50.3
(1.5)
P 		18.1
(-3)
A 		43
avr 53.2
(3) 		35.8
(-0.8) 		38.3
(-0.2) 		38.3
(-0.2) 		48.3
(1.9) 		25.4
(-3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 17
(1.2)
A 		9.6
(-1.7)
A 		21.5
(3)
M 		6.3
(-3)
A 		15
B 12.4
(0.4)
M 		16.2
(3)
M 		7.4
(-3)
P 		12.3
(0.3)
P 		9
C 20.8
(0.5)
P 		24.4
(1.8)
P 		27.4
(3)
A 		11.8
(-3)
P 		16
avr 16.7
(1.6) 		16.7
(1.6) 		18.8
(3) 		10.1
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 8.9
(-0.4)
P 		13.7
(2.6)
P 		9.7
(0.1)
P 		4.7
(-3)
A 		14.4
(3)
P 		11.1
(1)
M 		10
B 17.4
(2.3)
A 		7
(-2)
A 		8.2
(-1.5)
A 		15.7
(1.6)
A 		19
(3)
A 		4.7
(-3)
A 		14
C 22.6
(3)
A 		14.6
(0.1)
A 		9.3
(-1.8)
A 		7.5
(-2.5)
A 		16.3
(0.7)
P 		6.1
(-3)
A 		17
avr 16.3
(2.8) 		11.8
(-0.1) 		9.1
(-1.8) 		9.3
(-1.7) 		16.6
(3) 		7.3
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185804_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12890
Sequence Type Exemplar sequence
Representative Public ID Y32B12A.3 NCBI
Target Description Y32B12A.3 /REP_DB=WormBase Gene ID /WP=CE19060 /TR=Q9XXH0 /GB=CAA19479.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074774(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074774 NCBI Caenorhabditis elegans Y32B12A.3 (Y32B12A.3) mRNA, complete cds. 11/11 None
SNAP00000002059 ENSEMBL cdna:SNAP chromosome:CEL140:V:16495427:16497031:-1 11/11 None
GENEFINDER00000002063 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16496237:16497031:-1 11/11 None
Y32B12A.3 ENSEMBL cdna:known chromosome:CEL140:V:16496237:16497031:-1 gene:Y32B12A.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16496354-16497031(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y32B12A.3
Entrez Gene 189563 Entrez gene
SwissProt Q9XXH0 EMBL-EBI
WormBase CE36031 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8171 O-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA19479 Hypothetical protein Y32B12A.3 [Caenorhabditis elegans] ref|NP_507175.2| Y32B12A.3 [Caenorhabditis elegans] 1.0E-122
blast CAA19479 Hypothetical protein Y32B12A.3 [Caenorhabditis elegans] ref|NP_507175.2| Y32B12A.3 [Caenorhabditis elegans] 1.0E-119
blast AAK70657 Hypothetical protein Y40B10A.2 [Caenorhabditis elegans] ref|NP_503560.2| Y40B10A.2 [Caenorhabditis elegans] 3.0E-94
blast AAK70657 Hypothetical protein Y40B10A.2 [Caenorhabditis elegans] ref|NP_503560.2| Y40B10A.2 [Caenorhabditis elegans] 2.0E-91
blast CAE66789 Hypothetical protein CBG12149 [Caenorhabditis briggsae] 6.0E-89
Protein Domains
Database ID Description E-Value
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 4.1E-7
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 1.1E-30
Pfam IPR002935 EMBL-EBI O-methyltransferase, family 3 8.1E-30
Sequence
Target Sequence 
>C. ELEGANS:185804_AT
ccactatcgaatccgagctccaagctgagctccgccgagcaacaattgacagtgtcccaa
tggcaatgatacttggtgcgccggaagttttcacgtttggccagaacttcatcagatcct
tcggaggaaagagggtcctagatataggaacatttactggtgcttctgcattggcatggg
ctcttgctgttccagatggcactggggaagtgctcacttttgatataaatctcagcgatt
ataaaaagtacggagtccccataatttctaaatgcaaaaagactttcaaaaagattcgag
ctattgagggtcctgctttggagaatcttgacaaactcatcgccgatggccaatctggat
cttttgatttcgcattcattgacgccgacaagacaaactacagtaagtattacgatagat
gcgtgaccctcctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCACTATCGAATCCGAGCTCCAAGC 15 271 47 Antisense
AGCTCCGCCGAGCAACAATTGACAG 248 81 74 Antisense
GTGCGCCGGAAGTTTTCACGTTTGG 607 475 122 Antisense
GCCAGAACTTCATCAGATCCTTCGG 196 281 146 Antisense
GGAACATTTACTGGTGCTTCTGCAT 517 529 193 Antisense
TGCTTCTGCATTGGCATGGGCTCTT 435 579 207 Antisense
TACGGAGTCCCCATAATTTCTAAAT 179 647 295 Antisense
GAGCTATTGAGGGTCCTGCTTTGGA 688 387 344 Antisense
ATCGCCGATGGCCAATCTGGATCTT 235 37 385 Antisense
GATTTCGCATTCATTGACGCCGACA 494 427 412 Antisense
TTACGATAGATGCGTGACCCTCCTT 610 681 456 Antisense
Sequence Source Affymetrix Proprietary Database