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[185803_at]

Description C28C12.4 /REP_DB=WormBase Gene ID /WP=CE04111 /TR=Q18280 /GB=AAB37547.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 70      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 52      6grp_total > 03      4grp_total > 50      6grp_high1_sub > 04      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 895
1 2 3 4 range
A 229.4
(-2.2)
P 		918.4
(3)
P 		526.9
(0.1)
P 		117.3
(-3)
P 		801
B 207.6
(-2.2)
P 		897.2
(3)
P 		644.1
(1.1)
P 		98.6
(-3)
P 		799
C 203.4
(-2.2)
P 		978.7
(3)
P 		493.1
(-0.3)
P 		83.9
(-3)
P 		895
avr 213.5
(-2.2) 		931.4
(3) 		554.7
(0.3) 		99.9
(-3) 		832

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 662
1 2 3 4 5 6 range
A 816.1
(3)
P 		592.6
(0.6)
P 		555.4
(0.2)
P 		771.1
(2.5)
P 		622
(0.9)
P 		250.9
(-3)
P 		565
B 553.4
(-0.6)
P 		637
(0.2)
P 		671.2
(0.6)
P 		912.6
(3)
P 		571.9
(-0.4)
P 		317
(-3)
P 		596
C 787
(2.3)
P 		587
(0.1)
P 		410.4
(-1.9)
P 		851.9
(3)
P 		547.8
(-0.3)
P 		306.2
(-3)
P 		546
avr 718.8
(1.6) 		605.5
(0.4) 		545.7
(-0.2) 		845.2
(3) 		580.6
(0.1) 		291.4
(-3) 		554
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 267
1 2 3 4 range
A 83.5
(-0.8)
P 		199.4
(3)
P 		137.7
(1)
P 		17.2
(-3)
P 		182
B 66.2
(-1.6)
P 		221.6
(3)
P 		149.2
(0.8)
P 		21
(-3)
P 		201
C 67.3
(-1.8)
P 		281.4
(3)
P 		171.9
(0.5)
P 		14.2
(-3)
P 		267
avr 72.3
(-1.5) 		234.1
(3) 		152.9
(0.8) 		17.5
(-3) 		217

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 150.4
(0.6)
P 		210.8
(3)
P 		121
(-0.6)
P 		195.6
(2.4)
P 		187.7
(2.1)
P 		61.1
(-3)
P 		150
B 192.6
(0.6)
P 		212
(1.6)
P 		163
(-0.8)
P 		241.7
(3)
P 		232.4
(2.5)
P 		118.2
(-3)
P 		124
C 291.7
(3)
P 		179.1
(-0.6)
P 		104.5
(-2.9)
P 		171.3
(-0.8)
P 		177.2
(-0.6)
P 		102.5
(-3)
P 		189
avr 211.6
(3) 		200.6
(2.4) 		129.5
(-1.2) 		202.9
(2.6) 		199.1
(2.4) 		93.9
(-3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185803_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9044
Sequence Type Exemplar sequence
Representative Public ID C28C12.4 NCBI
Target Description C28C12.4 /REP_DB=WormBase Gene ID /WP=CE04111 /TR=Q18280 /GB=AAB37547.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069047(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069047 NCBI Caenorhabditis elegans C28C12.4 (C28C12.4) mRNA, complete cds. 11/11 None
SNAP00000012166 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8487501:8488316:1 11/11 None
GENEFINDER00000012179 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8487543:8488316:1 11/11 None
C28C12.4 ENSEMBL cdna:known chromosome:CEL140:IV:8487501:8488316:1 gene:C28C12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8487506-8488322(+) 72.18 100.0
Public Domain and Genome References
Gene Title
Ensembl C28C12.4
Entrez Gene 182978 Entrez gene
SwissProt Q18280 EMBL-EBI
WormBase CE04111 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37547 Hypothetical protein C28C12.4 [Caenorhabditis elegans] ref|NP_501448.1| C28C12.4 [Caenorhabditis elegans] 7.0E-67
blast AAB37547 Hypothetical protein C28C12.4 [Caenorhabditis elegans] ref|NP_501448.1| C28C12.4 [Caenorhabditis elegans] 6.0E-59
blast CAE71550 Hypothetical protein CBG18497 [Caenorhabditis briggsae] 1.0E-54
blast CAE71550 Hypothetical protein CBG18497 [Caenorhabditis briggsae] 6.0E-54
blast CAA93430 Hypothetical protein ZK596.1 [Caenorhabditis elegans] ref|NP_502026.1| ZK596.1 [Caenorhabditis elegans] 8.0E-27
blast CAA93430 Hypothetical protein ZK596.1 [Caenorhabditis elegans] ref|NP_502026.1| ZK596.1 [Caenorhabditis elegans] 9.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR003677 EMBL-EBI Protein of unknown function, DUF 148 5.1E-68
Pfam IPR003677 EMBL-EBI Protein of unknown function, DUF 148 5.1E-68
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501448.1 1 7-29
C28C12.4 1 7-29
SNAP00000012166 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:185803_AT
caggaggacgtggacgccacggaccaccaatgccaccattccttcaaaatgttactgatg
aaggaagacgtgcattcttcgatattgccagaaaccagaacttgacaattgctgagatgg
aatcacaaacttctacatgggctcagacttacggagtttcggatgtctactccgagttcg
aagctaacataaccgctcatcgcaacgaagttcaacaaaatgtaacccaagttgttagcc
aactctctgctgctcaaaccgctctcgaagcagtcatgaacaacaaaaaccagacccgcc
aacaaatgaaagaagccattgacaatctcaagacacaatatccacaagagattccagctc
tctttttcatcagtggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGGAGGACGTGGACGCCACGGACC 651 207 113 Antisense
GACGTGCATTCTTCGATATTGCCAG 593 377 179 Antisense
ATCACAAACTTCTACATGGGCTCAG 145 27 234 Antisense
GGGCTCAGACTTACGGAGTTTCGGA 483 491 251 Antisense
GGATGTCTACTCCGAGTTCGAAGCT 689 513 273 Antisense
GAAGCTAACATAACCGCTCATCGCA 312 343 292 Antisense
ACCGCTCATCGCAACGAAGTTCAAC 701 89 304 Antisense
AAGTTGTTAGCCAACTCTCTGCTGC 108 157 341 Antisense
CTCAAACCGCTCTCGAAGCAGTCAT 240 227 365 Antisense
AACCAGACCCGCCAACAAATGAAAG 290 137 400 Antisense
CCAGCTCTCTTTTTCATCAGTGGAT 278 273 466 Antisense
Sequence Source Affymetrix Proprietary Database