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[185784_at]

Description Y56A3A.32 /REP_DB=WormBase Gene ID /WP=CE22597 /TR=Q9U229 /GB=CAB60511.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 06      4grp_highest_8_8 > 18      4grp_high_8_8 > 61      6grp_highest_nonorm > 50      6grp_total > 06      4grp_total > 59      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 888
1 2 3 4 range
A 468.5
(-0.4)
P 		554.6
(0.6)
P 		766
(3)
P 		239.8
(-3)
P 		526
B 548.2
(-0.5)
P 		720.5
(0.7)
P 		1046
(3)
P 		201.1
(-3)
P 		845
C 561.3
(0.5)
P 		799.1
(2.6)
P 		847.7
(3)
P 		157.8
(-3)
P 		690
avr 526
(-0.1) 		691.4
(1.3) 		886.6
(3) 		199.6
(-3) 		687

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 347
1 2 3 4 5 6 range
A 564.1
(1.2)
P 		652.1
(3)
P 		381.5
(-2.5)
P 		522.7
(0.4)
P 		354.8
(-3)
P 		414.8
(-1.8)
P 		297
B 613.2
(3)
P 		422.9
(-1)
P 		383.3
(-1.8)
P 		419.5
(-1.1)
P 		414.9
(-1.2)
P 		328.6
(-3)
P 		285
C 665.1
(3)
P 		417.2
(-1.3)
P 		645.7
(2.7)
P 		356.9
(-2.3)
P 		462.4
(-0.5)
P 		317.9
(-3)
P 		347
avr 614.1
(3) 		497.4
(0.3) 		470.2
(-0.3) 		433
(-1.2) 		410.7
(-1.7) 		353.8
(-3) 		260
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 170.6
(1.9)
P 		120.4
(0.1)
P 		200.2
(3)
P 		35.1
(-3)
P 		165
B 174.8
(1)
P 		177.9
(1.1)
P 		242.4
(3)
P 		42.9
(-3)
P 		200
C 185.8
(0.6)
P 		229.7
(1.5)
P 		295.5
(3)
P 		26.6
(-3)
P 		269
avr 177.1
(1) 		176
(1) 		246
(3) 		34.9
(-3) 		211

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 175
1 2 3 4 5 6 range
A 104
(-2.2)
P 		231.9
(3)
P 		83.1
(-3)
P 		132.6
(-1)
P 		107
(-2)
P 		101
(-2.3)
P 		149
B 213.4
(3)
P 		140.8
(-0.6)
P 		93.1
(-3)
P 		111.1
(-2.1)
P 		168.6
(0.8)
P 		122.5
(-1.5)
P 		120
C 246.5
(3)
P 		127.3
(-1.1)
P 		164.3
(0.2)
P 		71.8
(-3)
P 		149.6
(-0.3)
P 		106.4
(-1.8)
P 		175
avr 188
(3) 		166.7
(1.5) 		113.5
(-2.4) 		105.2
(-3) 		141.7
(-0.4) 		110
(-2.7) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185784_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7134
Sequence Type Exemplar sequence
Representative Public ID Y56A3A.32 NCBI
Target Description Y56A3A.32 /REP_DB=WormBase Gene ID /WP=CE22597 /TR=Q9U229 /GB=CAB60511.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067163(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067163 NCBI Caenorhabditis elegans Worm AIF (apoptosis inducing factor) Homolog family member (wah-1) (wah-1) mRNA, complete cds. 11/11 None
SNAP00000035208 ENSEMBL cdna:SNAP chromosome:CEL140:III:11990076:11999320:1 11/11 None
GENEFINDER00000035231 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11990076:11999328:1 11/11 None
Y56A3A.32.1 ENSEMBL cdna:known chromosome:CEL140:III:11990037:11999468:1 gene:Y56A3A.32 11/11 None
Y56A3A.32.2 ENSEMBL cdna:known chromosome:CEL140:III:11990043:11999428:1 gene:Y56A3A.32 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11990071-11999324(+) 97.93 100.0
Public Domain and Genome References
Gene Title
Gene Symbol wah-1
Ensembl Y56A3A.32
Entrez Gene 176635 Entrez gene
SwissProt Q9U229 EMBL-EBI
WormBase CE31841 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604360_AT similar to programmed cell death 8 isoform 3 cfa
CANINE:1600558_AT similar to programmed cell death 8 isoform 3 cfa
CANINE_2:CFA.1525.1.A1_S_AT similar to programmed cell death 8 isoform 3 cfa
CANINE_2:CFA.17737.1.S1_S_AT similar to programmed cell death 8 isoform 3 cfa
DROSOPHILA_2:1626985_A_AT dm
DROSGENOME1:154947_AT dm
CHICKEN:GGA.11032.1.A1_AT programmed cell death 8 (apoptosis-inducing factor) gga
CHICKEN:GGAAFFX.13220.1.S1_S_AT programmed cell death 8 (apoptosis-inducing factor) gga
HG-U133_PLUS_2:205512_S_AT programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133A:205512_S_AT programmed cell death 8 (apoptosis-inducing factor) hs
HG-FOCUS:205512_S_AT programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133A_2:205512_S_AT programmed cell death 8 (apoptosis-inducing factor) hs
HU35KSUBA:RC_AA600310_AT programmed cell death 8 (apoptosis-inducing factor) hs
U133_X3P:G4757731_3P_A_AT programmed cell death 8 (apoptosis-inducing factor) hs
HG-U95AV2:32212_AT programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133_PLUS_2:216761_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133A_2:216761_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133A:216761_AT Programmed cell death 8 (apoptosis-inducing factor) hs
U133_X3P:HS.306750.0.S1_3P_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133_PLUS_2:216758_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133A_2:216758_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133A:216758_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133_PLUS_2:243353_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U133B:243353_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HG-U95B:44927_AT Programmed cell death 8 (apoptosis-inducing factor) hs
HU35KSUBC:RC_AA011379_AT Programmed cell death 8 (apoptosis-inducing factor) hs
U133_X3P:HS.271774.0.A1_3P_AT Programmed cell death 8 (apoptosis-inducing factor) hs
MU11KSUBA:AA138875_S_AT programmed cell death 8 mm
MG-U74AV2:96858_AT programmed cell death 8 mm
MOE430A:1418127_A_AT programmed cell death 8 mm
MOUSE430A_2:1418127_A_AT programmed cell death 8 mm
MOUSE430_2:1418127_A_AT programmed cell death 8 mm
MU19KSUBB:TC30876_AT Programmed cell death 8, mRNA (cDNA clone MGC:5706 IMAGE:3500037) mm
MU19KSUBC:TC37652_AT Programmed cell death 8, mRNA (cDNA clone MGC:5706 IMAGE:3500037) mm
RG-U34A:RC_AA891591_AT programmed cell death 8 rn
RAE230A:1370321_AT programmed cell death 8 rn
RAT230_2:1370321_AT programmed cell death 8 rn
RAE230B:1382871_AT Programmed cell death 8 rn
RAT230_2:1382871_AT Programmed cell death 8 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
15036 disulfide oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60511 Hypothetical protein Y56A3A.32 [Caenorhabditis elegans] ref|NP_499564.2| Worm AIF (apoptosis inducing factor) Homolog family member (wah-1) [Caenorhabditis elegans] 0.0
blast AAN60212 oxidoreductase WAH-1 [Caenorhabditis elegans] 0.0
blast CAE67755 Hypothetical protein CBG13330 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001327 EMBL-EBI FAD-dependent pyridine nucleotide-disulphide oxidoreductase 2.1E-31
Pfam IPR001327 EMBL-EBI FAD-dependent pyridine nucleotide-disulphide oxidoreductase 2.1E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499564.2 1 74-93
Y56A3A.32.1 1 74-93
Y56A3A.32.2 1 74-93
SNAP00000035208 1 74-93
GENEFINDER00000035231 1 83-102
Sequence
Target Sequence 
>C. ELEGANS:185784_AT
gatggctccgaacttcgaactgatctggttgtggtggcaactggtgaggagccgaattcc
gagattattgaggcttctggcctgaaaattgatgagaagcttggtggagttcgagctgat
aaatgcctgaaagttggtgaaaatgtgtgggcggctggtgcgattgcaacgtttgaggat
ggggttctcggtgcgagacgagtgagctcttgggagaatgcacagattagtggacgactg
gctggagagaatatggcgactgctgctgctgatgggaagtcggagggaaaagctttttgg
taccaaccatcattcttcaccaaattcgccccacatctgcatatcaacgcgatcggaaaa
tgcgattcctcactggaaaccgtatcagttcatgctgaaccagacaaggatacgcctctt
gagaaggctgtcgtattttataaatcgaaagaagatggaagtatcgtcggtgtcctactc
ctgaacgtgttcggaccttcactggatgtagctcggcgtataattgatgataggaagaaa
gtggatgagtacaaggaaattgcaaagcttttcccactttatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGGCTCCGAACTTCGAACTGATC 48 407 1321 Antisense
GTGAAAATGTGTGGGCGGCTGGTGC 424 481 1457 Antisense
TGAGGATGGGGTTCTCGGTGCGAGA 1 569 1494 Antisense
AGAGAATATGGCGACTGCTGCTGCT 552 67 1566 Antisense
TTGGTACCAACCATCATTCTTCACC 260 707 1617 Antisense
CCCACATCTGCATATCAACGCGATC 262 259 1650 Antisense
GCGATTCCTCACTGGAAACCGTATC 159 289 1682 Antisense
GATGGAAGTATCGTCGGTGTCCTAC 282 407 1774 Antisense
GTCCTACTCCTGAACGTGTTCGGAC 339 477 1792 Antisense
GTTCGGACCTTCACTGGATGTAGCT 177 441 1809 Antisense
ATTGCAAAGCTTTTCCCACTTTATG 557 5 1879 Antisense
Sequence Source Affymetrix Proprietary Database