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[185780_at]

Description Y50E8A.16 /REP_DB=WormBase Gene ID /WP=CE24404 /TR=Q9U275 /GB=CAB60586.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 16      4grp_high_8_8 > 09      6grp_total > 16      4grp_total > 23      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 198
1 2 3 4 range
A 177.6
(3)
P 		90.7
(-2.3)
P 		79.3
(-3)
P 		147.5
(1.2)
P 		98
B 157.4
(3)
P 		49.7
(-3)
A 		98
(-0.3)
P 		140.2
(2)
P 		108
C 247.2
(3)
P 		81.1
(-3)
P 		80.4
(-3)
P 		168.2
(0.2)
P 		167
avr 194.1
(3) 		73.8
(-3) 		85.9
(-2.4) 		152
(0.9) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 145.3
(3)
P 		139.5
(2.6)
P 		85.3
(-0.7)
P 		76.9
(-1.2)
P 		48.6
(-3)
A 		74.7
(-1.4)
P 		97
B 202.2
(3)
P 		120.4
(0)
P 		74.9
(-1.7)
P 		38.3
(-3)
A 		76.7
(-1.6)
P 		67.7
(-1.9)
P 		164
C 221.2
(3)
P 		147
(0.4)
P 		108.8
(-0.9)
P 		49.1
(-3)
A 		64.3
(-2.5)
P 		84.4
(-1.8)
P 		172
avr 189.6
(3) 		135.6
(0.6) 		89.7
(-1.4) 		54.8
(-3) 		63.2
(-2.6) 		75.6
(-2.1) 		135
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 64.7
(3)
P 		19.7
(-3)
P 		20.7
(-2.9)
P 		21.6
(-2.7)
P 		45
B 50.2
(3)
P 		12.3
(-3)
A 		22.7
(-1.4)
P 		29.9
(-0.2)
P 		38
C 81.8
(3)
P 		23.3
(-3)
P 		28
(-2.5)
P 		28.4
(-2.5)
P 		59
avr 65.6
(3) 		18.4
(-3) 		23.8
(-2.3) 		26.6
(-2) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 26.8
(-0.9)
P 		49.6
(3)
P 		18.6
(-2.3)
P 		19.5
(-2.2)
P 		14.7
(-3)
A 		18.2
(-2.4)
P 		35
B 70.4
(3)
P 		40.1
(-0)
P 		18.2
(-2.2)
P 		10.1
(-3)
A 		31.2
(-0.9)
P 		25.2
(-1.5)
P 		60
C 82
(3)
P 		44.9
(-0.1)
P 		27.7
(-1.5)
P 		9.9
(-3)
A 		20.8
(-2.1)
P 		28.3
(-1.5)
P 		72
avr 59.7
(3) 		44.9
(1.1) 		21.5
(-1.9) 		13.2
(-3) 		22.2
(-1.8) 		23.9
(-1.6) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185780_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13008
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.16 NCBI
Target Description Y50E8A.16 /REP_DB=WormBase Gene ID /WP=CE24404 /TR=Q9U275 /GB=CAB60586.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074244(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074244 NCBI Caenorhabditis elegans HAlF transporter (PGP related) family member (haf-7) (haf-7) mRNA, complete cds. 11/11 None
SNAP00000008991 ENSEMBL cdna:SNAP chromosome:CEL140:V:14775980:14779920:1 11/11 None
GENEFINDER00000009974 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14775980:14779920:1 11/11 None
Y50E8A.16 ENSEMBL cdna:known chromosome:CEL140:V:14775952:14779920:1 gene:Y50E8A.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14775979-14779920(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol haf-7
Ensembl Y50E8A.16
Entrez Gene 190115 Entrez gene
SwissProt Q9U275 EMBL-EBI
WormBase CE24404 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60586 Hypothetical protein Y50E8A.16 [Caenorhabditis elegans] ref|NP_506645.1| HAlF transporter (PGP related) family member (haf-7) [Caenorhabditis elegans] 0.0
blast T31617 hypothetical protein Y50E8A.m - Caenorhabditis elegans 0.0
blast CAE66359 Hypothetical protein CBG11616 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001140 EMBL-EBI ABC transporter, transmembrane region 6.6E-33
Pfam IPR003439 EMBL-EBI ABC transporter 1.0E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506645.1 7 9-31,51-73,86-108,118-137,232-254,365-387,443-465
Y50E8A.16 7 9-31,51-73,86-108,118-137,232-254,365-387,443-465
SNAP00000008991 7 9-31,51-73,86-108,118-137,232-254,365-387,443-465
GENEFINDER00000009974 7 9-31,51-73,86-108,118-137,232-254,365-387,443-465
Sequence
Target Sequence 
>C. ELEGANS:185780_AT
gaacattgaccatctccattatcacacaaacattgcactagttgcacaggaaccaacttt
attctctggaactgttcgagaaaatatcctttatggacttgaaaatggaacggatgagga
tatgatgcgtgtagctgaaatggcgaatgttcatgaattcgtgtctaaaatggagaaggg
atatgataccaagtgcggggaaaaaggagtccaaatgtctggaggacagaagcaaagaat
tgcaattgctcgagctttgatccgtgatccacgtgttctgatccttgacgaagccacatc
ggcgctggattcagagtctgaagctatggtacaagaggcgttaaatcgatgtgctcgtga
acgaactgtattggtgattgctcatcgtttatccactgtgagaagtgctgatcgaattgc
tgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACATTGACCATCTCCATTATCAC 283 351 1860 Antisense
ACCATCTCCATTATCACACAAACAT 162 87 1868 Antisense
GCACAGGAACCAACTTTATTCTCTG 254 317 1903 Antisense
AACTTTATTCTCTGGAACTGTTCGA 107 139 1914 Antisense
GAGCTTTGATCCGTGATCCACGTGT 494 387 2111 Antisense
TCCACGTGTTCTGATCCTTGACGAA 73 589 2127 Antisense
ACATCGGCGCTGGATTCAGAGTCTG 219 107 2155 Antisense
AAATCGATGTGCTCGTGAACGAACT 170 117 2202 Antisense
GAACGAACTGTATTGGTGATTGCTC 67 347 2218 Antisense
ATTGCTCATCGTTTATCCACTGTGA 249 7 2236 Antisense
GAGAAGTGCTGATCGAATTGCTGTA 685 391 2259 Antisense
Sequence Source Affymetrix Proprietary Database